Abstract:

Objective：To investigate the species of bacterial contamination of laboratory sheets. Methods：laboratory sheets were direct sampled and enrichment culture then inoculating blood Columbia agar plates and MacConkey Agar plates. Isolated bacteria were identified by morphology，Gram stain and biochemical test. Results：From 1 000 laboratory sheets，after inoculate，there were 719 bacteria growing in blood agar platein. The differential strains grew on blood Columbia agar plates and their infection rates were：Staphylococcus，518 strains，infection rates was 72.04%；Enteric bacilli，175 strains，infection rates was 24. 34%；Bacillus subtilis，26 strains，infection rates was 3.62%. The differential strains grew on MacConkey Agar plates and their infection rates were ：Staphylococcus，229 strains，infection rates was 81.03%；Enteric bacilli，70 strains，infection rates was 18.97%. Conclusion：In the inspection report，the main bacteria are mainly including Staphylococcus and Enterobacteriaceae. Staphylococcus is mainly Staphylococcus epidermidis or Staphylococcus aureus，and Enterobacteriaceae is mainly Yersinia or Shigella.

Key words: laboratory sheets, bacterial contamination, species