A Study of Genotyping in Mycobacterium tuberculosis Strains in Dali by VNTR Technique

Abstract

Abstract:

Objective: To explore DNA polymorphism and genotyping features of strains in Dali by investigating the genotyping in
Mycobacterium tuberculosis clinical isolates in Dali with variable number tandem repeats (VNTR) analysis. Methods: 3 VNTR loci of 60 Mycobacterium tuberculosis strains were tested with VNTR technology and cluster analysis was performed by Quantity one and BioNumerics 6.6. Results: 60 strains were categorized into 4 gene clusters (I, II, III and IV) and 28 genotypes. Cluster I accounted for 15.0% including 5 genotypes; Cluster II, 31.7% including 8 genotypes; Cluster III, 40.0% including 11 genotypes; Cluster IV, 13.3% including 4 genotypes. Conclusion: There was obvious DNA polymorphism of Mycobacterium tuberculosis clinical strains and Cluster III was the predominant prevalent strain in Dali.

Key words: Mycobacterium tuberculosis, genotyping, VNTR, polymorphism, Dali

CLC Number:

DONG Yi, WU Li-Xian. A Study of Genotyping in Mycobacterium tuberculosis Strains in Dali by VNTR Technique[J]. J4, 2014, 13(8): 23-25.

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