Table of Content

15 August 2014, Volume 13 Issue 8

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Chemical Constituents of Stellera chamaejasme

CHEN Jian-Yong, LIU Guang-Ming, ZHANG Yu, HAO Xiao-Jiang, HE Hong-Ping

2014, 13(8):  1-3. 

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Objective: To research the chemical constituents of Stellera chamaejasme. Methods: The constituents were isolated from the plant by silica gel column chromatography, C18 reversed phase chromatography, Sephadex LH-20 gel filtration chromatography and HPLC. Their structures were elucidated by spectroscopic methods. Results: Five known compounds were isolated and identified as (2R, 3S)- 3- (3, 4- dimethoxybenzyl)- 2- (3, 4- methylenedioxybenzyl)- butyrolactone (1), haplomyrfolin (2), dihydrosesamin (3), isoneochamaejasmine A (4) and ruixianglangdusu B (5), respectively. Conclusion: Compounds 1-3 were isolated from this plant for the first time.

Experimental Study on Acute Toxicity of Anti-HIV-1 Active Extracts from Pinecone of Pinus yunnanensis

SHI Zhi-Ting, LIU Xi, ZHANG Hai-Zhu

2014, 13(8):  4-6. 

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Objective: To study acute toxicity of anti-HIV-1 active extracts from pinecone of Pinus yunnanensis and to evaluate its
medicinal safety. Methods: The maximum dosage test was used, and after intragastric administration of pinecone extracts in mice (25 g/kg once for 14 days, with acute toxicity observed during this period,the body weight and organ weight were measured and the histopathological examination on the liver and kidney was conducted. Results: The acute toxicity test showed no toxicity, the control group and the treatment group showed no significant difference statistically on organ index and pathological examination showed no abnormal results in each group.Conclusion: Therewasnoacutetoxicityreactiononanti-HIV-1activeextractsfrompinecone.

Protective Effect of Periplaneta americana Extracts on Myocardial Ischemia Induced by
Isoproterenol in Rats

LU Li, ZHANG Xu-Qiang, GAN Ping, HE Xu, CHEN Jun-Ya, PENG Fang

2014, 13(8):  7-10. 

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Objective: To study the protective effect of CⅡ-3 extracted from Periplaneta americana on myocardial ischemia in rats
induced by isoproterenol (ISO). Methods: The myocardial ischemia model was made by using subcutaneous injection of ISO in rats.The change of S-T segment and Q-T interval of electrocardiogram (ECG) was observed, and the content of LDH, CK, SOD and MDA in serum was measured. The pathological lesion of myocardium were observed. Results: Three types of dosage of CⅡ-3（low, 25 mg/kg;mid, 50 mg/kg; high, 100 mg/kg）all significantly reduced S-T segment deviation and shortened Q-T interval(P < 0. 05). Middle and high dosage of CⅡ-3 significantly reduced the content of MDA( P < 0. 01) and raised the activity of SOD (P < 0. 05). High dosage of CⅡ-3 reduced the activity of CK (P < 0. 05), but three dosage of CⅡ-3 had the subtle effect on the activity of LDH. Pathological examination showed that three dosages of CⅡ-3 could reduce the pathological lesion of myocardium in rats to varying degrees. Conclusion: CⅡ-3 had some protective effect on myocardial ischemia in rats induced by ISO.

Effects of Grape Seed Proanthocyanidin Extract on Hepatic Ischemia Reperfusion
Oxidative Injury and Apoptosis

LIU Ren-Gui, ZHANG Zi-Hua, QIN Yan, SU Juan, YANG Ji-Wu

2014, 13(8):  11-14. 

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Objective: To study the effects of grape seed proanthocyanidin extract（GSPE）on antioxidation of hepatic ischemia
reperfusion injury in rats, and the expression of apoptosis- related proteins Bcl- 2 and Bax. Methods: Thirty SD male rats were randomly divided into three groups with 10 rats each .i.e. sham operation group, HIRI model group and GSPE preconditioning group （160 mg/kg）. The content of GSH-Px, SOD, and MDA in hepatic tissues, and the content of ALT and AST in serum were assayed 3 hours later after reperfusion. The morphological variations of hepatic tissues in each group were compared by means of optical microscope. Effects of GSPE on Bcl-2 and Bax in hepatic tissues were tested by means of immunohistochemisty. Results: The content of ALT and AST in serum and MDA in hepatic tissues decreased significantly（P＜0.05）, and the content of GSH-Px and SOD in hepatic tissues increased remarkably（P＜0.05）in GSPE preconditioning group, compared with HIRI model; Observed by the optical
microscope, the degree of liver cell injury in GSPE preconditioning group was remarkably slighter than that of HIRI model group. Immunohistochemical results showed that the content of Bcl-2 increased and Bax decreased significantly in hepatic tissues, compared with that in HIRI model（P＜0.05）. Conclusion: GSPE had some effects on antioxidation and anti-apoptosis of hepatic ischemia reperfusion injury.

Preliminary Study on the Antibacterial Effects and Mechanism of Opuntia monacantha Extracts in Dali

HUANG Yuan, ZHANG Peng-Cheng, YANG Kang, CHEN Dong-Ju, LIU Wen-Ying, ZHANG Lei

2014, 13(8):  15-17. 

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Objective: To study the antibacterial effects and mechanism in vitro of the ethanol extracts and ultrasonic extracts of
Opuntic monacantha in Dali. Methods: Ethanol extraction and ultrasonic extraction were respectively applied to the preparation of primary extracts of Opuntia monacantha, and antibacterial effects on experimental strains Staphylococcus aureus, Escherichia coli and Candida albicans were detected by using K-B method. The minimum inhibitory concentrations (MIC) were measured by the macro dilution method, and the antibacterial mechanism was preliminarily explored by the antibacterial curve. Results: There are inhibitory effects in two kinds of primary extracts on experimental strains. The MIC of ethanol extracts were lower than that of ultrasonic extracts. Antibacterial curve demonstrated that the ethanol extracts of Opuntic monacantha had antibacterial effects on Staphylococcus aureus
within 9 hours of growth. Conclusion: Two kinds of extracts of Opuntic monacantha in Dali were able to inhibit the growth of experimental strains including gram-negative and gram-positive bacteria and fungi; antimicrobial effects of ethanol extracts were better than those of ultrasonic extracts. This study could provide a theoretic basis for further research on and application of the extracts of Opuntia monacantha.

The Study of Auto-inducing Fermentation of Mycobacterium tuberculosis Protein MTB8.1

NIE Heng, GAO Zhi-Fang, WU Li-Xian

2014, 13(8):  18-22. 

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Objective: To explore an effective fermentation process of Mycobacterium tuberculosis protein MTB8.1 with soluble
expression, which would be suitable for industrial development. Methods: Strain pUC18/MTB8.1/DH10B was used as fermentation strain and a systematic study of the composition of medium and the parameters of fermentation was conducted to obtain a new fermentation process. Results: By means of the new fermentation process, the yield of soluble recombinant protein MTB8.1 was greatly increased in per unit volume of fermentation broth, with about 30 mg of soluble protein obtained from every liter of fermentation broth through purification. Conclusion: A better way of soluble expression of MTB8.1 in Escherichia coli expression system was found tentatively, which laid the theoretical foundation for the industrial production.

A Study of Genotyping in Mycobacterium tuberculosis Strains in Dali by VNTR Technique

DONG Yi, WU Li-Xian

2014, 13(8):  23-25. 

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Objective: To explore DNA polymorphism and genotyping features of strains in Dali by investigating the genotyping in
Mycobacterium tuberculosis clinical isolates in Dali with variable number tandem repeats (VNTR) analysis. Methods: 3 VNTR loci of 60 Mycobacterium tuberculosis strains were tested with VNTR technology and cluster analysis was performed by Quantity one and BioNumerics 6.6. Results: 60 strains were categorized into 4 gene clusters (I, II, III and IV) and 28 genotypes. Cluster I accounted for 15.0% including 5 genotypes; Cluster II, 31.7% including 8 genotypes; Cluster III, 40.0% including 11 genotypes; Cluster IV, 13.3% including 4 genotypes. Conclusion: There was obvious DNA polymorphism of Mycobacterium tuberculosis clinical strains and Cluster III was the predominant prevalent strain in Dali.

Research Advances in Transcription and Regulation Mechanism of Mammalian Mitochondrial Genome

XIONG Wei, ZHANG Hai-Yang, YANG Hong-Juan, DU Ya-Man

2014, 13(8):  26-30. 

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Objective: To summarize systematically recent research advances in transcription and regulation mechanism of
mammalian mitochondrial genome in order to provide reference for research on mammalian and human mitochondrial diseases and other related fields. Methods: Information retrieval and a summary of literature and research advances in mtDNA transcription and regulation mechanism were made from aspects of genome structure, transcription process, transcriptional elements and mechanism.Results: Mitochondria were semiautomous organelles which had independent genome found in mammalian cells. Their major function was providing ATP for cells by oxidative phosphorylation and they also played an important role in physiological processes such as
metabolism, the regulation of cell cycle, cell differentiation and apoptosis, and cell signaling. In recent years, the research on transcription and regulation mechanism of mitochondrial genome has made a breakthrough and progress. Conclusion: Research on transcription and regulation mechanism of mitochondrial genome is helpful not only in expounding and understanding its mechanism underlying the expression regulation but also in revealing pathogenesis of clinic mitochondrial diseases.

Value Analysis of Noninvasive Positive Pressure Ventilation in the Treatment of Preterm Infants with
Respiratory Distress Syndrome

LI Mei-Jun, ZHANG Hong

2014, 13(8):  31-32. 

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Objective: To analyze clinical efficacy of nasal continuous positive airway pressure ventilation（CPAP）in the treatment
of preterm infants with respiratory distress syndrome（NRDS）and explore the value of CPAP for NRDS preterm infants. Methods:Based on combined therapy, CPAP was adopted for 84 cases with RDS from Oct. 2012 to Oct. 2013 at the neonatal department of the Maternal and Child Health Care Hospital of Dali Prefecture with the neonatal gestation age between 32 and 36 weeks. The efficacy was observed before the adoption of CPAP and six hours after the adoption of CPAP. Results: Six hours after the adoption of CPAP, cyanosis disappeared, inspiratory dyspnea was alleviated, the respiratory rate dropped to（58.2±4.7）/min, the heart rate dropped to （142.8±9.4）/min, fraction of inspired oxygen was（42.0±7.3）%, percutaneous oxygen saturation remained（94.8±4.5）%, the arterial
partial pressure of oxygen fell to（8.7±0.7）kPa and arterial partial pressure of carbon dioxide fell to（5.3±0.6）kPa, which showed significant difference. There was no other obvious complication except that the condition of 2 cases aggravated and therefore invasive mechanical ventilation was adopted. Conclusion: CPAP had a good curative effect on RDS preterm infants and the side effect was not serious and the earlier the treatment begun, the higher the cure rate and success rate were. Therefore, CPAP was worthy of introduction and promotion in primary hospitals.

A Study of Correlation between Intelligence and Behavior of Learning Disabled Children

YI Jun

2014, 13(8):  33-35. 

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Objective: To investigate correlation between intelligence and behavior of children with learning disabilities. Methods:
208 cases（IQ＞70）with average scores below the tenth percentile in the class for 2 consecutive years in observation group, 200 cases in the control group with average scores of middle or higher level and the same age with the observation group. Both groups were tested and evaluated with Achenbach Child Behavior Checklist（CBCL）, Wechsler Intelligence Scale for Children-Revised（WISC-R）and self-designed questionnaires. Results: There was significant difference（P＜0.001）between full intelligence quotient（FIQ）, verbal intelligence quotient（VIQ）and performance intelligence quotient（PIQ）of the observation group and the control group. Subtests of
block（P＜0.002）and jigsaw（P＜0.005）also showed significant difference between the two groups（P＜0.001）. Conclusion: There existed unbalanced development of intelligence structure, often accompanied by psychological and behavioural problems among learning disabled children. The society, teachers and parents should accept them psychologically and give them more care and understanding so that they could grow up healthy.

The Application of Draf Frontal Sinus Drainage Surgery in the Treatment of Frontal Recess Lesions

CHEN Yin-Zhong, GUO Qing-Hua, DING Yue-Ming

2014, 13(8):  36-38. 

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Objective: To explore the feasibility of Draf frontal sinus drainage surgery in the treatment of frontal recess lesions.
Methods: Draf frontal sinus drainage surgery was undergone in 12 patients with frontal recess lesions. Results: All patients' frontal sinuses were opened completely in the surgery and followed up for more than 6 months and no postoperative primary diseases occurred, only one case with frontal recess blocking. Conclusion: Draf frontal sinus drainage surgery could open frontal sinus safely and effectively.

Observation of the Clinical Effect of ⁹⁰Sr—⁹⁰Y Applicator in 245 Verruca Vulgaris Patients

YIN Xiu-Hui, JIN Ping-Yan, LIN Ming, SUN Tian-Ming

2014, 13(8):  39-40. 

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Objective: To explore the clinical effect of adopting ⁹⁰Sr—⁹⁰Y to treat verruca vulgaris. Methods: ⁹⁰Sr—⁹⁰Y applicator,
developed by Department of Isotope, China Institute of Atomic Energy, was uesed to treat 245 verruca vulgaris patients by irradiating.Results：The cure rate was 100% after 1-3 courses. Conclusion：The therapy of ⁹⁰Sr—⁹⁰Y applicator was safe, simple, painful and economical so it was worthy of clinical application.

Investigation and Analysis of the Prevalence Rate of Hospital Infection in a Provincial Hospital in Yunnan
from 2008 to 2012

LI Jun-Feng, CAO Lan-Fang, LAN Mei-Hua, SHI Xiao-Yuan, ZOU Nan-Qian

2014, 13(8):  41-44. 

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Objective: To evaluate the changing trend in the hospital infection transection investigation results from 2008 to 2012.
Methods: The prevalence rate of hospital infection during the five years was compared and analyzed. Results: The difference in the prevalence rate of infection in 2008 was statistically significant（P＜0.05）, compared with the year 2009, 2010, 2011 and 2012. The prevalence rate of infection in upper respiratory tract declined from 2.28% to 1.06%（P＜0.01）, lower respiratory tract declined from 2.85% to 1.82%（P＜0.01）, urinary tract declined from 1.48% to 0.53%（P＜0.01）, and surgical site increased from 0.34% to 1.06%（P＜0.01）. The usage rate of antimicrobial agents declined significantly from 49.60% to 22.31%（P＜0. 01）. The constituent ratio of single antimicrobial usage increased from 80.23% to 88.10%（P＜0.01）, while the constituent ratio of two combined and three combined antimicrobial usage declined significantly（P＜0. 05）. The ratio of bacterial culture rose sharply from 7.67% to 60.91%（P＜0. 01）. Conclusion: The infection control work in the hospital had produced remarkable positive effects.

Investigation of Serological Phenotype and Irregular Antibody among Volunteer Blood Donors of
Rh（D）Negative in Dali Prefecture

LI Jian-Bo, DU Yue-E, DIAO Li-Bo, CAI Yun-Ling, YE Wen-Hua, LIANG Yan-Jiao, HE Yong-Xun

2014, 13(8):  45-46. 

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Objective: To investigate serological phenotype and to detect irregular antibody among Rh（D）negative blood donors in Dali Prefecture. Methods: 136 803 blood samples were detected to determine the Rh phenotypes through serological tests and the irregular antibodies were screened through antiglobulin tests. Results: 209 blood samples were Rh（D）negative（0.15%）. The irregular antibodies were found in one Rh（D）negative sample（0.48%）. The serological phenotypes included 123 ccdee（58.85%）, 67 Ccdee（32.06%）, 7 CCdee（3.35%）, 6 ccdEe（2.87%）, 5 CcdEe（2.39%）and 1 CcdEe（0.48%）. Among all Rh（D）negative volunteer blood donors, the distribution of A, B, O and AB blood types was A＞O＞B＞AB. Conclusion: The study of serological phenotype and irregular antibody of Rh（D）negative blood donors would be helpful for enhancing the clinical safety and timely blood
transfusion.

Analysis of Mycoplasma Infection Situation and Drug Sensitivity of Female Genital Tract in Mangshi

YANG Shu-Yuan, LIU Hou-Chang

2014, 13(8):  47-48. 

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Objective: To understand mycoplasma infection and drug resistance situations of female genital tract of Mangshi.
Methods: 932 female cervical secretions samples were analyzed respectively for mycoplasma culture and drug sensitivity. Results:636 mycoplasma cases out of 932 were detected, whose positive rate was 68.2%（636/932）. Females with single infection of Ureaplasma urealyticum（Uu）were 447 cases, whose positive rate was 48.0%（447/932）. Females with single infection of Mycoplasma hominis（Mh）were 8 cases, whose positive rate was 0.9%（8/932）. 82 cases were tested out with Uu and Mh mixed infection, whose positive rate was 19.5%（182/932）. Conclusion: Local female genital tract mycoplasma infection rate was higher, which were mainly
caused by Uu. The resistant rate of ciprofloxacin and erythromycin were higher, so it was recommended to use josamycin and doxycycline.

Translational Medicine: the Inevitable Developing Trend of Modern Medicine

SHEN Yuan-Ying

2014, 13(8):  49-51. 

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Objective: To discuss the inevitable trend, research prospect and the role of translational medicine in modern medicine.Methods: Large numbers of latest studies on translational medicine at home and abroad were consulted and summarized. Results: The essence of translational medicine is the combination of theory with practice, which requires multidisciplinary overlap and cooperation so as to realize the two-way idea from laboratory to clinical application and from clinical application to the laboratory. Conclusion: Translational medicine is the inevitable trend of modern medical development and a new starting point and focus in the world's medical research.

The Analysis of the Health Status of the Occupational Population of a Cement Enterprise in Dali

LI Li

2014, 13(8):  52-54. 

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Objective: To evaluate the health status of the workers of a cement company in Dali so as to find the occupational
contraindication and diseases. Methods: Analysis of the occupational physical examination data about 1 219 workers（n=1 219）of a cement enterprise in Dali in 2012. Results: There were 19 workers with suspected occupational diseases and 25 with occupational contraindication. Clinic indexes showed 587 workers had 951 abnormal items, which accounted for 48.15%. Among all abnormal items about circulation system, 224 items were about hypertension with the highest percentage（18.38%）, 157 items about hyperlipidemia with the second highest percentage（12.88%）, followed by fatty liver, liver function, routine blood test, electrocardiogram and liver hemangioma. The difference was statistically significant（χ2 =519，P＜0.01）Among 587 workers with abnormal items, were 467 male
（51.43%）and 120 female（38.59%）. Statistical analysis showed the difference was statistically significant（χ2 =15.31，P＜0.01）. Conclusion: The health status of workers in the cement company could not be neglected and the corresponding measures should be taken. The government department of work safety must strengthen the supervision of occupational health in enterprises and urge them to improve the occupational health status.

Detection and Analysis of the Pollution of Fluorescent Whitening Agent in Tissue Paper Products in the
Dali Market

TAO Wen-Ling, CHEN Zheng, XIA Peng, ZHU Ai-Li, LIANG Feng-Mei, HU Yun-Ping, LI You

2014, 13(8):  55-56. 

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Objective: To know the contamination status of fluorescent whitening agent in tissue paper products in the Dali market and provide references for the supervision of tissue paper products to protect people's health. Methods: Fluorospectrophotometry was used to determine the fluorescent whitening agent and the data was analyzed by SPSS16.0. Results: The content of the fluorescent whitening agent in different qualities of tissue paper was different and the difference was statistically significant. The dissolution rate of the fluorescent whitening agent was 82.38% in soaked paper lunch boxes. Conclusion: There was the pollution of fluorescent whitening agent in tissue paper products in Dali. Therefore, it was necessary to strengthen the supervision and protect the people's health.

The Application of Capillary Gas Chromatography in Determination of Sodium Cyclamate in Milk

YANG Sheng-Chun, ZHANG Li-Gang, ZHOU Ping, HE Li-Xian

2014, 13(8):  57-59. 

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Objective: To study the analysis method of capillary gas chromatography in determination of sodium cyclamate content in milk and to lower the detection limit of the method by optimizing the settling agent dosage, esterification time and other conditions.Methods: Sodium cyclamate reacted in the medium of sulfuric acid with sodium nitrite and cyclohexanol nitrite was produced and extracted with n-hexane, and its content was determined by capillary gas chromatography. Results: Linear range was from 0.05 to 0.4 mg/mL, the detection limit was 2 μg, recovery of standard addition was 99.4%-102.5%, and RSD was 1.4%. Conclusion: The method was simple, rapid and accurate, with good reproducibility and low detection limit, much better than detection limit of 4 μg by GB/T5009.97-2003 method.

Isolation and Identification of Cryptococcus neoformans from Pigeon Droppings in Dali

TAO Xing-Chen- , LIU Xiao-Ying, SHANG Qiu-Ju- , YANG Jing- , SU Hong-Yan

2014, 13(8):  60-62. 

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Objective: To investigate the positive rate of Cryptococcus neoformans in pigeon droppings in Dali and provide a basis for effectively avoiding its infection. Methods: A black rice culture medium was used for isolation and identification of Cryptococcus neoformans in 140 pigeon droppings. Results: 46 strains of Cryptococcus neoformans were isolated and urea tests all proved positive.Conclusion: The positive rate of Cryptococcus neoformans in pigeon droppings in Dali was very high（32.9%）.

Application of Risk Management in Nursing Management in Tumor Hospital

QIN Ya-Hui

2014, 13(8):  63-65. 

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Objective: To strengthen the nursing risk management, refine the nursing system and improve nurses' consciousness of risk and prevention. Methods: A series of risk management measures were implemented, such as analyzing risk factors,strengthening the risk consciousness, carrying out the training of "professional knowledge, theories and skills", and adopting the management of "fixed nursing post, personalized nursing content and humanized whole nursing course", emphasizing "the important time period, patient, and observation" and making responsibilities, system and monitoring all in place. Results: Nurses' risk consciousness was improved remarkably. In addition, nursing quality and patients' satisfaction increased, errors and accidents decreased greatly, 26 wards passed the inspection of "High Quality Nursing Service Demonstration Project" and nursing service built up a reputation for the hospital. Conclusion: Improving the consciousness of risk prevention, establishing an effective risk management mechanism and attaching importance to detail management were three basic and critical factors.

The Nursing of Artificial Airway of Patients with Severe Craniocerebral Injury Using Mechanical
Ventilation

YANG Jian-Fei, LI Hong-Mei

2014, 13(8):  66-67. 

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Objective: To explore the nursing of artificial airway of patients with severe craniocerebral injury using mechanical
ventilation. Methods: A retrospective review of the nursing of artificial airway of 58 ICU cases treated in our hospital from August 2011 to August 2012 was made. Results: Strengthening the nursing of artificial airway of patients with severe craniocerebral injury using mechanical ventilation improved the success rate of rescuing patients with severe craniocerebral injury. Conclusion: Good nursing care of artificial airway was the key to successful treatment of the patients with severe craniocerebral injury.

Nursing Care for HIV/AIDS Patients with Colostomy during Perioperative Period

ZHAO Yu-Ping

2014, 13(8):  68-70. 

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Objective: To explore and summarize preoperative and postoperative psychological intervention and enterostomy methods concerning 8 HIV/AIDS patients who underwent colostomy after radical resection of rectal carcinoma. Methods: The preoperative education of AIDS knowledge, guidance on protective measures and postoperative observation and care of colostomy. Results: Among these patients, incisions of 7 cases healed in Phase I , incision of 1 case healed well in Phase II after active treatment. The patients and their relatives accepted the surgery and colostomy and grasped the colostomy nursing skills. Conclusion: Patient and careful colostomy nursing instruction can make patients acquire colostomy nursing skills, relatives learn right protective measures and accept AIDS patients, improve the life quality of AIDS patients and their confidence in life. The current effect is satisfactory.

Nursing Intervention of Bilicardiac Reflex in Biliary Tract Operations

WANG Yan-Shu, ZHAO Yuan

2014, 13(8):  71-73. 

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Objective: To explore effective prevention and nursing intervention of bilicardiac reflex in biliary tract operations.
Methods: Extensive studies and literature were consulted, combined with the author's own clinical nursing experience. Results:Preoperative complete physical assessment, the choice of anesthetic methods, the use of drugs, elimination of mental factors such as tension; intraoperative selection of incision, calmness of patients' nerves, good intraoperative coordination and timely and effective treatment of bilicardiac reflex were critical measures to ensure patient safety. Conclusion: Preoperative, intraoperative and postoperative comprehensive and effective nursing intervention could prevent the occurrence of the bilicardiac reflex in the biliary tract operations to a maximum degree and improve the quality of clinical care.

Observation of the Cooling Effects between Ice Blanket and Ice Blanket Supplemented by Medicine on
Patients with Central High Fever

YANG Xiu-Zhang

2014, 13(8):  74-75. 

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Objective: To observe the cooling effects of ice blanket supplemented by anal administration of indometacin suppositories on patients with central high fever. Methods: In addition to conventional treatment, two cooling methods i.e. ice blanket and ice blanket supplemented by anal administration of indometacin suppositories, were adopted, and their effects on patients were compared. Results: The difference in cooling effects between two methods was statistically significant（P＜0.05）. Conclusion: The cooling effects of ice blanket supplemented by anal administration of indometacin suppositories were apparently better than those of ice blanket alone.

Application of Quality Tracing Information System to Aseptic Materials

WANG Sheng-Xin

2014, 13(8):  76-78. 

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Objective: To take full advantage of sterilized items quality traceability system in the sterilization and supply center and
integrate the processing of aseptic materials into information management system to improve professional management of hospital sterilization and supply. Methods: Using bar code technology, combined with traceability management system software, to give omplete, real-time process traceability management to the processing of reusable instruments. Results: The work in the sterilization and supply center became more efficient, accurate and convenient and the sterilization quality of reusable instruments was ensured.Conclusion: The application of quality tracing information system to aseptic materials in the sterilization and supply center enabled a comprehensive trace of every detail of the work and accurate analysis of the problem and thus, to a certain extent, reduce the occurrence of medical disputes.

The Application of Nursing Risk Management in General Surgery Wards

HEI Xi-Sheng, LIU Zhong-Yuan

2014, 13(8):  79-81. 

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Objective：To explore the nursing risk management in general surgery wards and its effect. Methods: Nurses' risk
consciousness and abilities to prevent risks were improved by the following measures such as implementing the Four Key quality management strategies, perfecting nursing risk management system, reinforcing legal awareness and improving service attitude,increasing communication skills and mutual trust between doctors and patients. Results: That nurses' awareness of risk and service were increased could significantly improve the quality of nursing. Conclusion: The application of nursing risk management in general surgery wards improved the patients' satisfaction and the quality of nursing, guaranteed the security and reduced disputes in nursing.

Carrying out a Series of Student Activities about Nurse and Building up a New Stage of Nursing Education

CHAI Shi-Xue

2014, 13(8):  82-85. 

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Nursing College of Dali University has been adhering to the philosophy of "caring for patients, sharing health" since its
establishment, and has carried out a series of activities around the International Nurses Day, such as Cap Granting Ceremony, Nursing Skills Competition, Cuisine Competition in English, Theatrical Festival, Selection of Sunshine Girls, and so on. These activities have increased students' professional andpractical capabilities, and benefited the cultivation of advanced nursing professionals with good knowledge, ability and quality.

Speaking with Pictures：the Experience of Making Dermatovenereology Multimedia Courseware

HUANG Jing, HUANG Ling

2014, 13(8):  86-87. 

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Making full use of pictures in multimedia courseware in dermatovenereology teaching is a good way to make teaching more interesting and effective.

Attempt at Improving the Teaching Quality of the Elective Course of Health and Massage

DUAN Yi-Mei

2014, 13(8):  88-90. 

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The Health and Massage course is very popular with college students. In the teaching with limited periods, various ways can be adopted to improve the teaching quality, such as introducing a variety of teaching methods, placing emphasis on confusing and complex points, creating the classroom atmosphere, checking strictly on the practice sequence, strengthening the training in basic skills, teaching students how to protect themselves and promoting the practice and operative skills.

Survey on Parents' Sense of Well-being in Nursery Period

ZHOU Li-Qiong, TANG Cai-Run, DONG Cheng-Yan, ZHANG Jian-Ting, WU Jing, YANG Xian-Lei, JI Shou-Lian

2014, 13(8):  91-92. 

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Objective: To investigate the status of subjective well- being sense of parenting parents in some areas of Yunnan
province. Methods: Issuing questionnaires about status of subjective well-being to survey 400 parenting couples' sense of well-being status. Results: The rating differences of satisfaction and positive emotions dimensions of nursaery parents show statistical significance （P<0.05）；comparing each couples' sense of well-being and couples in urban and rural aeras，the rating difference is statistically significant（P<0.05）；rating differences also show statistical significance（P< 0.05）by comparing influencing factors such as gender，rural-urban disparity，health status，educational level，interpersonal relationship and income differences of surveyed nursery couple.
Conclusion: Subjective well-being sense of parenting parents is in the state of satisfaction. Harmonious family atmosphere should be well-established in order to improve subjective well-being of nursery parents.