大理大学学报 ›› 2023, Vol. 8 ›› Issue (8): 1-5.

• 药学 •    下一篇

绒毛灯笼花块根化学成分研究

李 靖1,2,凌浩威1,2,史凯旋1,2,刘 蓉1,2,肖朝江1,2,姜 北1,2*   

  1. (1.云南省滇西抗病原植物资源筛选研究重点实验室,云南大理  671000;2.大理大学药学院,云南大理  671000)

  • 收稿日期:2023-01-09 修回日期:2023-02-14 出版日期:2023-08-15 发布日期:2023-07-11
  • 通讯作者: 姜北,教授,博士,E-mail:jiangbei@dali.edu.cn。
  • 作者简介:李靖,硕士研究生,主要从事天然产物研究。
  • 基金资助:
    国家自然科学基金项目(81960637);云南省滇西抗病原植物资源筛选研究重点实验室项目(202105AG070003);云南省教育厅科学研究基金项目(2022Y822)

Study on Chemical Constituents of the Root of Agapetes lacei var. tomentella

Li Jing1,2, Ling Haowei1,2, Shi Kaixuan1,2, Liu Rong1,2, Xiao Chaojiang1,2, Jiang Bei1,2*   

  1. (1. Yunnan Key Laboratory of Screening and Research on Anti-Pathogenic Plant Resources from Western Yunnan, Dali, Yunnan 671000, China; 2. College of Pharmacy, Dali University, Dali, Yunnan 671000, China)

  • Received:2023-01-09 Revised:2023-02-14 Online:2023-08-15 Published:2023-07-11

摘要: 目的:研究绒毛灯笼花Agapetes lacei var. tomentella干燥块根中的化学成分。方法:采用硅胶柱色谱和薄层色谱法对绒毛灯笼花块根75%乙醇提取物乙酸乙酯部位进行分离纯化,依据理化性质及现代波谱技术对化合物进行结构鉴定。结果:从绒毛灯笼花块根75%乙醇提取物乙酸乙酯部位中分离得到10个化合物,分别鉴定为:3α,23-O-异亚丙基-2α,19α-二羟基-12-烯-28-乌苏酸(1)、3β,23-O-异亚丙基-2α,3β,23-三羟基-12-烯-28-齐墩果酸(2)、2α,3α,19α,23-四羟基-12-烯-28-乌苏酸(3)、2α,3β,23-三羟基-12-烯-28-齐墩果酸(4)、3β-乙酰乌苏酸(5)、乌苏酸(6)、β-谷甾醇(7)、5α,6β-二羟基谷甾醇(8)、胡萝卜苷(9)、4-羟基丁酸(10)。结论:所有化合物均为首次从绒毛灯笼花中分离得到,除化合物7和9外,其余化合物均为首次从树萝卜属植物中分离得到。

关键词: font-family:宋体, ">font-family:宋体, ">绒毛灯笼花, 树萝卜属, 化学成分, 三萜, 甾体

Abstract: Objective: To study the chemical constituents of the dried root of Agapetes lacei var. tomentella. Methods: Silica gel column chromatography and thin layer chromatography were used to isolate and purify the ethyl acetate fraction of the 75% ethanol extract of the root of A. lacei var. tomentella, and the chemical structures of the compounds were identified based on physicochemical properties and modern spectroscopic techniques. Results: Ten compounds were isolated from the ethyl acetate fraction of the 75% ethanol extract of the root of A. lacei var. tomentella and identified as 3α,23-O-isopropylidenyl-2α,19α-dihydroxyurs-12-en-28-oic acid(1), 3β, 23-O-isopropylidenyl-2α,3β,23-trihydroxyolean-12-en-28-oic acid(2), 2α,3α,19α,23-tetrahydroxyurs-12-en-28-oic acid(3), 2α,3β,23-trihydroxyolean-12-en-28-oic acid(4), 3β-acetoxy ursolic acid(5), ursolic acid(6), β-sitosterol(7), 5α,6β-dihydroxysitosterol(8), daucosterol(9), and 4-hydroxybutanoic acid(10). Conclusion: All the compounds were isolated from A. lacei var. tomentella for the first time, except for compounds 7 and 9, the other compounds were first isolated from genus Agapetes.

Key words: font-family:Times New Roman, ">Agapetes lacei var. tomentellafont-family:Times New Roman, ">, font-family:Times New Roman, "> Agapetesfont-family:Times New Roman, ">, font-family:Times New Roman, "> chemical constituentsfont-family:Times New Roman, ">, font-family:Times New Roman, "> triterpenoidsfont-family:Times New Roman, ">, font-family:Times New Roman, "> steroids

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