Protective Effect of Xinmailong Capsules on Morphologic Injury in Primary Cultured Rat Vascular Smooth Muscle Cells

J4 ›› 2012, Vol. 11 ›› Issue (9): 29-31.

Protective Effect of Xinmailong Capsules on Morphologic Injury in Primary Cultured Rat Vascular Smooth Muscle Cells

Objective: To investigate the primary culture method of rat aorta VSMCs and observe the protection of XMLJ on
morphologic injury of VSMCs indcued by H₂O₂. Methods: Rat aorta VSMCs were cultured following tissue-piece inoculation method. Cultured VSMCs were then stimulated with 62.5 μmol·L^-1 H₂O₂under the condition of with or without XMLJ, HE dyeing method was applied to observe the morphologic changes of VSMCs. Results: With XMLJ 200 μg·mL^-1, VSMCs under oxidative stress exhibited nearly no difference compared with the normal cells. XMLJ with 200 μg·mL^-1 on VSMCs morphologic change induced by oxidative stress showed obvious protective effect.Conclusion: Tissue-piece inoculation culture of VSMCs is uncomplicated. There was a protective effect of XMLJ on morphologic injury of VSMCs.

College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China

Received:2012-03-23 Revised:2012-04-11 Online:2012-09-15 Published:2012-09-15

Abstract

Abstract:

Key words: rat, vascular smooth muscle cells, tissue-piece inoculation, oxidative stress, cellular morpology

CLC Number:

R285.5

XIAO Fen, WU Jian-Xin. Protective Effect of Xinmailong Capsules on Morphologic Injury in Primary Cultured Rat Vascular Smooth Muscle Cells[J]. J4, 2012, 11(9): 29-31.

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