Study on Identification of the Microbial Strain for Transforming Puerariae Radix and Its Microbial
Characteristics

Abstract

Abstract:

A strain of filamentous fungi which was able to transform the active ingredient in Puerariae radix was obtained in microbial
transformation experiments and named YM3207. After observing and analyzing the morphology and ITS sequence of this fungi, it was
identified as Monascus purpureus. HPLC was used to analyze the change of transformation column. The content of Genistein and
Puerarin have been improved. At the same time, antibacterial activity enhanced and antimicrobial spectrum expanded as well.

Key words: Puerariae radix, microbial transformation, identification, antibacterial

CLC Number:

Q939.5：R28

Song Yanqiu, Chen Youwei. Study on Identification of the Microbial Strain for Transforming Puerariae Radix and Its Microbial
Characteristics[J]. J4, 2015, 14(12): 64-68.

References

〔1〕LOUGHLIN W A. Biotransformation in organic synthesis〔J〕.
Bioresource Technol，2000（74）：49-62.
〔2〕占纪勋，钟建江，戴均贵，等. 红豆杉愈伤组织中紫杉烷
类成分sinenxan A 的微生物转化研究〔J〕. 药学学报，
2003，38（7）：555-558.
〔3〕唐燕红，张日俊. 微生物在中草药添加剂中的应用研究
进展〔J〕. 中国饲料，2004（3）：20-21.
〔4〕肖培根. 新编中药志：卷1〔M〕. 北京：化学工业出版社，
2001：962-974.
〔5〕范秀容，李广武，沈萍. 微生物学实验〔M〕. 北京：高等教
育出版社，1989：260-269.
〔6〕李绍兰，周斌，杨丽媛，等. 真菌DNA 提取方法的改
良〔J〕. 云南大学学报（自然科学版），2002，24（6）：471-
472.
〔7〕THOMPSON J D，GIBSON T J，PLEWNIAK F，et al. The
Clastal X Windows interface：flexible strategies for multiple
sequence alignment aided by quality analysis tools〔J〕.
Nucleic Acids Research，1997（24）：4876-4882.
〔8〕JEANNMOUGIN F，THOMPSON J D，GOUY M，et al.
Multiple sequence alignment with Clustal X〔J〕. Trends
Biochemistry Science，1998（23）：403-405.
〔9〕KUMAR S，TAMURA K，JAKOBSEN I B，et al. MEGA2：
Molecualr revolutionary genetics analysis software 〔J〕.
Bioinformatics，2001（17）：1244-1245.
〔10〕COMERON J M. A method for estimating the numbers of
synonymous and nonsynonymous substitutions per site〔J〕.
Journal of Molecular Evolution，1995（41）：1152-1159.
〔11〕李绍平，李萍，季晖，等. 天然与发酵培养冬虫夏草中核
苷类成分的含量及其变化〔J〕. 药学学报，2001，36（6）：
436.
〔12〕戴芳澜. 真菌的形态和分类〔M〕. 北京：科学出版社，
1987：1-352.
〔13〕魏景超. 真菌鉴定手册〔M〕. 上海：上海科学技术出版
社，1979：1-780.
〔14〕金文姗，谈钰元，陈有根，等. 高效液相色谱法测定不同
产地葛根中葛根素、大豆苷及大豆苷元的含量〔J〕. 中
国中药杂志，2003，28（1）：49-51.
〔15〕张蕾，朱蓉贞，潘扬，等. 不同品种及产地的葛根中葛根素
含量的比较〔J〕. 中国中药杂志，1995，20（7）：399-400.
〔16〕谢明杰，陆敏，邹翠霞，等. 大豆异黄酮的抑菌作用〔J〕.
大豆科学，2004，23（2）：101-105.
〔17〕井乐刚，张永忠，田璐. 大豆异黄酮抑菌活性的研
究〔J〕. 哈尔滨师范大学自然科学学报，2004，20（1）：
79-81.

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