Journal of Dali University ›› 2023, Vol. 8 ›› Issue (10): 21-26.

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The Inhibitory Activity of Pinus yunnanensis Pine Extract with Anti-HIV Activity against Human Coronavirus 229E

Li Yafei1,2,Shang Fangjian1,2,Luo Chunyu1,2,Shi Zhefang3,Liu Qi1,2*   

  1. (1.Pre-clinical College, Dali University, Dali, Yunnan 671000, China; 2.Yunnan Provincial Key Laboratory of Insect Biomedical Research and Development, Dali, Yunnan 671000, China; 3. Baoding Hospital of Beijing Children's Hospital, Capital Medical University, Baoding, Hebei 071000, China)

  • Received:2023-03-20 Revised:2023-04-01 Online:2023-10-15 Published:2023-10-26

Abstract:

Objective: To evaluate the inhibitory activity of Pinus yunnanensis pine extract(referred to as "pine extract") with anti-HIV activity against human coronavirus HCoV-229E and preliminarily determine its target. Methods: A cell-cell fusion model and  model mediated by HCoV-229E S protein were constructed to detect the inhibitory activity of pine extract against HCoV-229E. Time-of-addition and time-of-removal experiments were performed to determine the target and the timing of action. Results: Pine extract could inhibit the cell-cell fusion mediated by HCoV-229E S protein, with an IC50 of (0.136±0.010) mg/mL. It effectively inhibited HCoV-229E infection, with an IC50 of (0.069±0.015) mg/mL. Time-of-removal and time-of-addition experiments showed that pine extract targeted the HCoV-229E S protein and acted in the early stage of infection(1 h before); different concentrations of pine tower extracts have no significant toxicity to 293T and Huh-7 cells. Conclusion: Pine extract can target the HCoV-229E S protein and inhibit HCoV-229E infection in the early stage, making it a safe candidate drug.

Key words:

Pinus yunnanensis, pine extract, HCoV-229E, inhibitory activity

CLC Number: