Abstract: Objective： To study the chemical constituents of the dried root of Agapetes lacei var. tomentella. Methods： Silica gel column chromatography and thin layer chromatography were used to isolate and purify the ethyl acetate fraction of the 75% ethanol extract of the root of A. lacei var. tomentella， and the chemical structures of the compounds were identified based on physicochemical properties and modern spectroscopic techniques. Results： Ten compounds were isolated from the ethyl acetate fraction of the 75% ethanol extract of the root of A. lacei var. tomentella and identified as 3α，23-O-isopropylidenyl-2α，19α-dihydroxyurs-12-en-28-oic acid（1）， 3β， 23-O-isopropylidenyl-2α，3β，23-trihydroxyolean-12-en-28-oic acid（2）， 2α，3α，19α，23-tetrahydroxyurs-12-en-28-oic acid（3）， 2α，3β，23-trihydroxyolean-12-en-28-oic acid（4）， 3β-acetoxy ursolic acid（5）， ursolic acid（6）， β-sitosterol（7）， 5α，6β-dihydroxysitosterol（8）， daucosterol（9）， and 4-hydroxybutanoic acid（10）. Conclusion： All the compounds were isolated from A. lacei var. tomentella for the first time， except for compounds 7 and 9， the other compounds were first isolated from genus Agapetes.

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