产β-内酰胺酶金黄色葡萄球菌的耐药性监测

J4 ›› 2010, Vol. 9 ›› Issue (2): 16-18.

产β-内酰胺酶金黄色葡萄球菌的耐药性监测

1.大理学院附属医院，云南大理 671000；2.云南省地方病防治所，云南大理 671000

收稿日期:2009-12-28 出版日期:2010-02-15 发布日期:2010-02-15
作者简介:周浓，讲师，主要从事中药资源与品质评价研究.
基金资助:
国家自然科学基金资助项目（30860365）

Monitoring of Antimicrobial Resistance of Staphylococcus Aureus Strains Producing β- lactamase

1. Affiliated hospital, Dali University, Dali, Yunnan 671000, China;
2. Yunnan Institute for Endemic Disease Control and Prevention, Dali, Yunnan 671000, China

Received:2009-12-28 Online:2010-02-15 Published:2010-02-15

摘要/Abstract

摘要：

目的：探讨大理地区产β-内酰胺酶金黄色葡萄球菌的耐药情况。方法：采用VITEK-32型全自动微生物鉴定仪将葡萄球
菌属鉴定到种，头孢硝噻吩滤纸片法检测β-内酰胺酶，药物敏感试验采用K-B法。结果：61株金黄色葡萄球菌中产β-内酰胺酶13株，产酶率21.3%，产酶株对万古霉素最敏感，其次为左氧氟沙星，对其他抗生素的耐药率前四位分别是氨苄西林92.4%，青霉素G92.4%，红霉素84.7%，克林霉素69.3%。结论：金黄色葡萄球菌的茁内酰胺酶检测应作为常规项目开展，产β-内酰胺酶的金黄色葡萄球菌对青霉素类，大环内酯类抗生素高度耐药，万古霉素无耐药菌株，为首选药物，应在药敏试验指导下合理选用抗生素。

关键词: 金黄色葡萄球菌, β内酰胺酶, 耐药性监测

Abstract:

Objective: To investigate the characteristics of antimicrobial resistance of Staphylococcus aureus strains producing β-
lactamase which were isolated from clinic in local hospitals. Methods: By using VITEK-32 automated microbiology system for identification of Staphylococcus, β- lactamase in Staphylococcus aureus was detected by Nitrocefin paper. Antimicrobial susceptibility was detected by the disk diffusion method. Results: Of 61 strains of Staphylococcus aureus, 13 strains produce β-lactamase and the rate of producing enzymes was 21.3%. The strains producing enzymes were most sensitive to Vancomycin and more sensitive to Levofloxacin. The resistant rate of ampicillin and pencillin G were 92.4% and erythromycin was 84.7%; clindamycin was 69.3%. Conclusions: The detection of β- lactamase in Staphylococcus aureus must be a normal item. The strains producing β- lactamase were highly resistant to penicillins and macrolides. Vancomycin must be the first choice in anti-infection caused by Staphylococcus aureus. Antibiotics should be chosen rationally under the guide of the microbial sensitivity test.

Key words: staphylococcus aureus, β- lactamase, drug resistance

中图分类号:

R378.1

张群智, 张红英, 忽胜和, 孔山. 产β-内酰胺酶金黄色葡萄球菌的耐药性监测[J]. J4, 2010, 9(2): 16-18.

ZHANG Qun-Zhi, ZHANG Hong-Ying, HU Sheng-He, KONG Shan. Monitoring of Antimicrobial Resistance of Staphylococcus Aureus Strains Producing β- lactamase[J]. J4, 2010, 9(2): 16-18.

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