酸性染料比色法测定千里光总生物碱含量

J4 ›› 2011, Vol. 10 ›› Issue (12): 30-31.

酸性染料比色法测定千里光总生物碱含量

目的：研究测定千里光中总生物碱含量的方法。方法：以氧化苦参碱为对照品，采用酸性染料比色法，在422 nm 波长处测定千里光中总生物碱含量。结果：氧化苦参碱在6.75~135 μg 与吸光度呈良好的线性关系（r=0.999 2），平均回收率100.0%（RSD= 1.54%）。结论：该方法简便、准确，可用于千里光中总生物碱的测定。

大理学院药学与化学学院，云南大理 671000

收稿日期:2011-04-28 修回日期:2011-09-17 出版日期:2011-12-15 发布日期:2011-12-15
作者简介:张丽虹，大理学院药学与化学学院本科生.
基金资助:
大理学院大学生科研基金（DXSKYX201012）

Determination of Total Alkaloids Content in Senecio Scandens Buch.-Ham. by Acid Dye Colorimetry

Objective: To determine the total alkaloids content in Senecio scandens Buch.-Ham. Methods: Using the oxymatrine as standard, acid dye colorimetry was used to determine the total alkaloids content in S. scandens at the wavelength of 422 nm. Results:The concentration of oxymatrine showed good linearity in the range of 6.75-135 μg （r=0.999 2）, the average recovery was 100.0%（RSD=1.54%）. Conclusion: The method is simple, reliable and can be used for determination of the total alkaloids in S. scandens.

School of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China

Received:2011-04-28 Revised:2011-09-17 Online:2011-12-15 Published:2011-12-15

摘要/Abstract

摘要：

关键词: 酸性染料比色法, 千里光, 总生物碱

Abstract:

Key words: acid dye colorimetry, Senecio scandens Buch.-Ham., total alkaloids

中图分类号:

R286
R917

张丽虹, 杨玉萍, 陈小花, 肖婷婷, 肖培云. 酸性染料比色法测定千里光总生物碱含量[J]. J4, 2011, 10(12): 30-31.

ZHANG Li-Hong, YANG Yu-Ping, CHEN Xiao-Hua, XIAO Ting-Ting, XIAO Pei-Yun. Determination of Total Alkaloids Content in Senecio Scandens Buch.-Ham. by Acid Dye Colorimetry[J]. J4, 2011, 10(12): 30-31.