TGF—β1与EGF对肺泡Ⅱ型上皮细胞表型及功能的影响

J4 ›› 2011, Vol. 10 ›› Issue (6): 17-20.

TGF—β1与EGF对肺泡Ⅱ型上皮细胞表型及功能的影响

目的：探讨TGF-β1与EGF对肺泡Ⅱ型上皮细胞(RLE-6TN)的影响。方法：将细胞分成对照(c)、TGF-131(T)、EGF(E)及TGF-131+EGF(TE)组。采用相差显徽镜观察细胞形态，免疫荧光检测α-SMA表达，w．B检测上皮、间质细胞标记物、MMPs及信号蛋白的表达；流式细胞术检测凋亡情况。结果：T及TE组RLE-6TN发生EMT转变并见间质标记物及MT1-MMP、MMP2表达上调，E组Vimentin、MT1-MMP、MMP2及CD147表达上调；T与E组p-ERK、p-38MAPK及p-Akt表达上调，T组p-Smad2表达上调；T与E组均见凋亡，TE组凋亡加剧。结论：单用EGF不能诱导RLE-6TN发生EMT，合用TGF-β1无协同诱导EMT，却能协同诱导凋亡。TGF-p1诱导EMT时，主要通过EGFR信号通路诱导MMPs表达。

1．大理学院基础医学院，云南大理 671000；
2．上海交通大学附属第六人民医院，上海 200233； 3．复旦大学上海医学院，上海 200032

出版日期:2011-06-01 发布日期:2011-06-01
通讯作者: 许祖德，教授，电话：021—54237596
作者简介:徐国萍，副教授，主要从事肺纤维化研究．
基金资助:
大理学院博士基金资助项目(KY430340)

Efect of TGF-β1 and EGF on the Phenotypical Change and Function of Type II Alveolar Epithelial Cells

1．Pre-clinical College，Dali University，Dali，Yunnan 671000，China； 2．Shanghai Jiaotong University No．6 People’s Hospital Shanghai 200233，China； 3．Shanghai Medical College，Fudan University，Shanghai 200032，China

Online:2011-06-01 Published:2011-06-01

摘要/Abstract

摘要：

关键词: ]TGF一131, EGF, 肺泡Ⅱ型上支细胞, EMT

Key words: TGF-&beta, 1, EGF, type lI alveolar epithelial cells, EMT

中图分类号:

R363

. TGF—β1与EGF对肺泡Ⅱ型上皮细胞表型及功能的影响[J]. J4, 2011, 10(6): 17-20.

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