HIV 相关性口腔白色念珠菌rDNA 基因型研究

J4 ›› 2011, Vol. 10 ›› Issue (8): 24-26.

HIV 相关性口腔白色念珠菌rDNA 基因型研究

目的：了解HIV 感染人群和健康人群口腔白色念珠菌基因型的分布情况。方法：运用CA-INT 特异性引物聚合酶链反应
方法扩增白色念珠菌包含1 类内含子的编码rRNA的25S rDNA 区，根据扩增条带大小进行基因分型，并应用χ²检验对54 株
HIV 感染人群和54 株健康人群口腔白色念珠菌基因型别进行分析。结果：CA-INT特异性引物PCR法可将108 株口腔白色念
珠菌分为A、B 和C型，以A型最多见，两组人群口腔白色念珠菌基因型分布基本相同，但HIV 感染人群口腔白色念珠菌A 型
所占构成比高于健康人群，差别有统计学意义。结论：HIV感染人群和健康人群口腔白色念珠菌均具有基因多态性，CA-INT 特异性引物PCR 法是较好的口腔白色念珠菌种内菌株间鉴定的方法。

大理学院基础医学院，云南大理 671000

收稿日期:2011-06-09 出版日期:2011-08-15 发布日期:2011-08-15
作者简介:张云操，硕士研究生，主要从事病原生物学研究.
基金资助:
国家自然科学基金资助项目（C30260005）；大理学院高层次人才基金资助项目（KY430440）

rDNA Genotyping of Oral Candida albicans Isolated from HIV-infected Population

School of Basic Medicine, Dali University, Dali, Yunnan 671000, China

Received:2011-06-09 Online:2011-08-15 Published:2011-08-15

摘要/Abstract

摘要：

关键词: 白色念珠菌, HIV, 聚合酶链反应, rDNA 基因型

Abstract:

Objective: To investigate the distribution of genotypes of oral Candida albicans isolated from HIV-related group and
healthy carriers group. Methods: PCR with CA-INT primers was designed to amplify group 1 intron-containing region in 25S rDNA of oral Candida albicans, the different strains of oral Candida albicans were classified into genotypes on the basis of bands from amplicons. Then the genotypic distribution results of all oral Candida albicans were analyzed with SPSS 17.0 by chi-square test. Results: One hundred and eight strains of oral cavities Candida albicans were classified into genotype A, B and C. Genotype A was the most predominant one. Identical genotypes distribution appeared in both groups. But the constituent ratio of genotype A of oral Candida albicans strains in HIV-positive group was statistically higher compared to the healthy people group. Conclusion: Genotypes of oral Candida albicans in both groups were highly polymorphic. The method in text is suitable to apply in genotyping research of oral Candida albicans.

Key words: Candida albicans, HIV, PCR, rDNA genotyping

中图分类号:

R378.99

张云操, 刘奇, 武有聪, 郭利军, 申元英, 白丽. HIV 相关性口腔白色念珠菌rDNA 基因型研究[J]. J4, 2011, 10(8): 24-26.

ZHANG Yun-Cao, LIU Qi, WU You-Cong, GUO Li-Jun, SHEN Yuan-Ying, BAI Li. rDNA Genotyping of Oral Candida albicans Isolated from HIV-infected Population[J]. J4, 2011, 10(8): 24-26.

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