J4 ›› 2012, Vol. 11 ›› Issue (3): 28-30.
目的：克隆福氏志贺菌（Shigella flexneri，S. flexne）的ipaB基因。方法：以福氏志贺菌2a 2457T（Nalr）为模板，用PCR扩增ipaB目的基因片段，克隆至pQE-30表达载体中，构建含目的基因的表达质粒pQE-ipaB。结果：构建了重组质粒pQE-ipaB，ipaB基因全长1 743 bp，经测序分析与预期相符。结论：成功克隆了ipaB基因。
Objective：To clone and sequence of ipaB gene of Shigella flexneri. Methods：IpaB gene was amplified by PCR. The DNA products of ipaB were inserted into a prokaryotic expression vector pQE-30, and then translated into E.coli strain Top10, restriction digestion and sequencing. Results：The recombinant plasmid was constructed and ipaB gene was sequenced as 1 743 bp, conformity with expectation. Conclusion：The results indicated that recombinant plasmid was constructed successfully.
