HDAC1、8在肺泡型上皮细胞间质转化中的表达及意义

J4 ›› 2013, Vol. 12 ›› Issue (3): 16-19.

HDAC1、8在肺泡型上皮细胞间质转化中的表达及意义

1.大理学院临床医学院，云南大理 671000；2.大理学院基础医学院，云南大理 671000

收稿日期:2013-10-15 出版日期:2013-03-15 发布日期:2013-03-15
作者简介:姜巍，硕士研究生，主要从事外科学研究.
基金资助:
国家自然科学基金青年科学基金资助项目（81100045）

Expression and Significance of Histone Deacetylase 1 , 8 in Alveolar Epithelial Type II Cells Mesenchymal Transition

1.Clinical College, Dali University, Dali, Yunnan 671000, China; 2. Pre-clinical College, Dali University, Dali, Yunnan 671000, China

Received:2013-10-15 Online:2013-03-15 Published:2013-03-15

摘要/Abstract

摘要：

目的：观察TGF-β1作用下，肺泡域型上皮细胞RLE-6TN在EMT过程中HDAC1、8的表达情况及TSA对TGF-β1诱导细胞
EMT的影响。方法：将TGF-β1加入到体外培养的细胞中，于不同时间收取细胞，采用WB及Real-time RT-PCR检测HDAC1、8及E-cad、α-SMA的表达情况。然后将TGF-β1加入经过TSA预处理的细胞中，于不同时间收取细胞，重新检测上述基因。结果：加入TGF-β1后，E-cad蛋白表达下调；α-SMA蛋白表达上调；HDAC1 蛋白表达上调；HDAC8蛋白表达下调。E-cad mRNA表达下调；α-SMA及HDAC8的mRNA均于12 h表达上调，6 h、24 h表达下调；HDAC1 mRNA表达于6 h下调，24 h上调。加入TSA后，E-cad蛋白表达上调；α-SMA、HDAC1及HDAC8蛋白的表达均下调。E-cad mRNA表达上调；α-SMA mRNA表达于6 h、12 h上调，24 h下调；HDAC1 mRNA表达下调；HDAC8 mRNA表达上调。结论：TGF-β1体外诱导的RLE-6TN细胞EMT，可以通过应用TSA抑制其获得α-SMA表型、失去E-cad表型，从而部分逆转TGF-β1诱导的肺泡域型上皮EMT。

关键词: HDAC1, TGF-β1, TSA, 肺泡域型上皮细胞, EMT

Abstract:

Objective: To observe the expression of histone deacetylase 1,8 on the process of TGF-β1 induced type II alveolar
epithelial cells to EMT, and investigate the effect of TSA on TGF-β1-induced the cells EMT process. Methods: RLE-6TN cell lines were cultured and treated with TGF-β1, and collected cells at different time points. The expression of markers and mRNA of E-cad，α-SMA，HDAC1 and HDAC8 were assayed by Western Blot and Real-time RT-PCR, respectively. Then add TGF-β1 to the cells after TSA pretreatment for 6 hours and repeat the above assay. Results: With the treatment of TGF-β1, the expression of E-cad protein was down regulated; α-SMA, HDAC1 and HDAC8 protein was unregulated. E-cad mRNA was down regulated; both the mRNA of α-SMA and HDAC8 were up regulated at 12 h, and were down regulated at 6 h, 24 h; HDAC1 mRNA at 6 h were down regulated, 24 h was up regulated. After pretreated with TSA, the expression of E-cad protein was up regulated; α-SMA, HDAC1 and HDAC8 protein was down
regulated. E-cad mRNA was up regulated; α-SMA mRNA at 6 h, 12 h was up regulated, 24 h was down regulated; HDAC1 mRNA was down regulated; HDAC8 mRNA was up regulated. Conclusion: It is possible to use TSA inhibited RLE-6TN cells to receive α-SMA phenotype, loss of E-cad phenotype, which partially reverse the EMT of RLE-6TN induced by TGF-β1 in vitro.

Key words: HDAC1, TGF-β1, TSA, alveolar epithelial type II cells, EMT

中图分类号:

R563.1⁺³

姜巍, 徐国萍. HDAC1、8在肺泡型上皮细胞间质转化中的表达及意义[J]. J4, 2013, 12(3): 16-19.

JIANG Wei, XU Guo-Ping. Expression and Significance of Histone Deacetylase 1 , 8 in Alveolar Epithelial Type II Cells Mesenchymal Transition[J]. J4, 2013, 12(3): 16-19.

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