J4 ›› 2013, Vol. 12 ›› Issue (3): 3-5.

• 药学 • 上一篇    下一篇

桑姜感冒胶囊中总黄酮含量的测定

  

  1. 大理学院药学与化学学院,云南大理 671000
  • 收稿日期:2012-11-30 出版日期:2013-03-15 发布日期:2013-03-15
  • 作者简介:朱坤宁,2010级药学专业本科生.
  • 基金资助:

    大理学院大学生科研基金资助项目(KYSX2011071)

Quantitative Determination of Total Flavonoids in Sangjiang Ganmao Capsule

  1. College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China
  • Received:2012-11-30 Online:2013-03-15 Published:2013-03-15

摘要:

目的:建立桑姜感冒胶囊中总黄酮含量的测定方法。方法:采用紫外-可见分光光度法,以芦丁为对照品,以506 nm作为
测量波长进行测定。结果:芦丁在0.5~3.0 mg范围内与吸光度呈良好的线性关系(r=0.999 7),平均回收率为96.63%,RSD为1.91%。结论:该方法操作简便、准确、重现性好,可用于测定桑姜感冒胶囊中总黄酮的含量。

关键词: 桑姜感冒胶囊, 总黄酮, 紫外-可见分光光度法

Abstract:

Objective: To establish UV spectrophotometry method for the determination total flavonoids in compounds of Sangjiang Ganmao capsule. Methods: The contents of the total flavonoids were determined at 506 nm with rutin as the standard substance by ultraviolet UV-visible method. Results: The calibration curve was linear in the range of 0.5-3.0 mg for rutin, r=0.999 7; the average recovery was 96.63%, RSD=1.91%(n=6). Conclusion: This method is simple, fast and accurate, and can be used as the determination of the total flavonoids in compound Sangjiang Ganmao capsule.

Key words: Sangjiang Ganmao capsule, total flavonoids, UV-visible method

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