›› 2016, Vol. 1 ›› Issue (12): 50-52.

• 化学 • 上一篇    下一篇

普洱茶水浸液对脱矿牙釉质再矿化的扫描电镜观察

  

  1. (大理大学药学与化学学院,云南大理671000)
  • 收稿日期:2016-02-29 修回日期:2016-04-11 出版日期:2016-12-15 发布日期:2016-12-15
  • 作者简介:杨华甫,2012级药物制剂专业本科生.
  • 基金资助:

    大理大学大学生科研基金资助项目(KYSX20155096)

Scanning Electron Microscopic Observation of the Remineralization Effect of Pu'er Tea Aqueous Extract
on Demineralized Enamel

  1. (College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China)
  • Received:2016-02-29 Revised:2016-04-11 Online:2016-12-15 Published:2016-12-15

摘要:

目的:观察普洱茶生茶、熟茶不同浓度水浸提取液处理人工龋后的表面形态,为天然防龋药物的研究与开发提供理
论依据。方法:利用化学试剂人工培养釉质脱矿模型,以水为提取液分别提取生茶、熟茶96、48、24、12、6 mg/mL为实验溶
液,20 mg/mL氟化钠为阳性对照,去离子水为阴性对照,共分为12组,经体外pH循环后在扫描电镜下观察。结果:生茶组和熟
茶组均随着浓度的增加牙釉质表面的沉积物越多越均匀致密,达96 mg/mL时有大量规则的结晶生成,熟茶组比生茶组的沉积
物更多更厚。氟化钠组大量的固体物质沉积,但结晶不紧密;去离子水组也有少许稀疏的沉积物,表面不平整,和脱矿后的差
别不大。结论:普洱茶生茶和熟茶水提取液均对人工脱矿釉质有再矿化作用,但低浓度效果不明显。

Abstract:

Objective: To observe the surface of artificial caries that treated by different concentration of raw and ripe Pu'er tea
aqueous extracts for providing a theoretical basis for the research and development of natural anti- caries drug. Methods:
Demineralizated enamel cultured by chemical reagents was used as model. With water as the extract, raw and ripe tea with 96,48,24,
12,6 mg/mL were used as test solution. 20 mg/mL NaF as the positive control group and deionized water as the negative control group
were divided into 12 groups and observed by scanning electron microscopy after pH extracorporeal circulation. Results: With the
increasing concentration of raw and ripe tea, the sediments on enamel surface were more uniform and compact and a large number of
regular crystallization generated when the concentration reached up to 96 mg/mL, and ripe tea group had more and thicker sediments
than raw tea; NaF group also had a lot of sediments, but was not compact; deionized water group only had a few sparse sediments with
uneven surface, and had little difference after demineralization. Conclusion: Raw and ripe Pu'er tea aqueous extract had the
remineralization effect on artificial demineralized enamel, but the low concentration was ineffective.

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