基底前脑巢蛋白阳性胆碱能神经元抗损伤作用

J4 ›› 2016, Vol. 1 ›› Issue (8): 35-38.

基底前脑巢蛋白阳性胆碱能神经元抗损伤作用

（大理大学基础医学院，云南大理671000）

收稿日期:2016-02-26 出版日期:2016-08-15 发布日期:2016-08-15
作者简介:王缓缓，硕士研究生，主要从事神经生物学研究.
基金资助:
国家自然科学基金资助项目（31160221）

Damage Resistance of Basal Forebrain Nestin-positive Cholinergic Neurons

（Pre-clinical College, Dali University, Dali, Yunnan 671000, China）

Received:2016-02-26 Online:2016-08-15 Published:2016-08-15

摘要/Abstract

摘要：

目的：探索基底前脑巢蛋白阳性和阴性胆碱能神经元对神经毒性损伤的不同反应。方法：30只SD大鼠随机分成5个
组，即对照组和秋水仙碱Ⅰ、Ⅱ、Ⅲ、Ⅳ组（10、20、50、100 μg）。分别经侧脑室注射10 μL生理盐水或不同剂量的秋水仙碱，24 h
后4%多聚甲醛灌注取脑，冰冻切片，行胆碱乙酰转移酶和巢蛋白免疫组化。计数和分析基底前脑不同区域巢蛋白阳性和阴性
胆碱能神经元的数目。结果：侧脑室秋水仙碱注射后，大鼠基底前脑不同区域巢蛋白阳性和阴性胆碱能神经元数目都下降。
注射剂量越大，神经元数目下降越明显。与巢蛋白阳性神经元相比，巢蛋白阴性胆碱能神经元下降出现更早，下降更明显。结
论：巢蛋白表达可增强基底前脑胆碱能神经元的抗损伤作用。

关键词: 基底前脑, 巢蛋白, 胆碱能神经元, 神经可塑性

Abstract:

Objective: To explore the different responses of basal forebrain nestin positive and negative cholinergic neurons to the
neurotoxicity. Methods: 30 SD rats were randomly divided into 5 groups, which are control group and colchicine Ⅰ,Ⅱ,Ⅲ,Ⅳ groups
（10, 20, 50, 100 μg）. 10 microliters of normal saline or different doses of colchicine were injectedintracerebroventricularly. 24 hours
after injection, 4% paraformaldehyde intracardiac perfusion, brain removal, frozen section, choline acetyltransferase and nestin
immunohistochemistry were carried out. Then, the nestin positive and negative cholinergic neurons of different basal forebrain regions
were counted and analyzed. Results: After intracerebroventricular injection of colchicine, the nestin positive and negative cholinergic
neurons in different regions of rats' basal forebrain decreased. The greater the colchicine injection dose is, the more obvious the
decrease of the neurons. Compared with nestin positive neurons, the nestin negative cholinergic neurons decreased earlier and more
significantly. Conclusion: the nestin expression can enhance the damage resistant ability of basal forebrain cholinergic neurons.

Key words: basal forebrain, nestin, cholinergic neurons, neural plasticity

中图分类号:

R322.81

王缓缓，朱建华. 基底前脑巢蛋白阳性胆碱能神经元抗损伤作用[J]. J4, 2016, 1(8): 35-38.

Wang Huanhuan, Zhu Jianhua. Damage Resistance of Basal Forebrain Nestin-positive Cholinergic Neurons[J]. J4, 2016, 1(8): 35-38.

参考文献

〔1〕GU H，WANG S，MESSAM C A，et al. Distribution of nes?
tin immunoreactivity in the normal adult human fore?
brain〔J〕. Brain Research，2002，943（2）：174-180.
〔2〕WANG S，YAO Z，WANG J，et al. Evidence for a distinct
group of nestin-immunoreactive neurons within the basal
forebrain of adult rats〔J〕. Neuroscience，2006，142（4）：
1209-1219.

〔3〕ZHU J，GU H，YAO Z，et al. The nestin-expressing and
non-expressing neurons in rat basal forebrain display differ?
ent electrophysiological properties and project to hippocam?
pus〔J〕. BMC Neuroscience，2011，12（1）：129.
〔4〕GUO K H，ZHU J H，YAO Z B，et al. Chemical identifica?
tion of nestin-immunoreactive neurons in the rat basal fore?
brain：a re-examination〔J〕. Neurochem Int，2010，56（5）：
694-702.
〔5〕PAXINOS G，WATSON C. The Rat Brain in stereotaxic co?
ordinates〔M〕. San Diego：Elsevier Academic Press，2007.
〔6〕朱建华，郭开华，徐杰. 金雀异黄酮对去卵巢大鼠基底前
脑一氧化氮合酶神经元的影响〔J〕. 中国临床康复，2005，
9（5）：36-37.
〔7〕LIEM R K. Molecular biology of neuronal intermediate fila?
ments〔J〕. Curr Opin Cell Biol，1993，5（1）：12-16.
〔8〕LENDAHL U，ZIMMERMAN L B，MCKAY R D. CNS stem
cells express a new class of intermediate filament protein〔J〕.
Cell，1990，60（4）：585-595.
〔9〕DOETSCH F，GARCÍA-VERDUGO J M，AlVAREZ-BUYL?
LA A. Cellular composition and three-dimensional organi?
zation of the subventricular germinal zone in the adult mam?
malian brain〔J〕. J Neurosci，1997，17（13）：5046-5061.
〔10〕GAGE F H，KEMPERMANN G，PALMER T D，et al. Mul?
tipotent progenitor cells in the adult dentate gyrus〔J〕. J
Neurobiol，1998，36（2）：249-266.
〔11〕MOKRÝ J，NEMECEK S. Angiogenesis of extra- and in?
traembryonic blood vessels is associated with expression
of nestin in endothelial cells〔J〕. Folia Biol（Praha），
1998，44（5）：155-161.
〔12〕SAHIN K S，MAHMOOD A，LI Y，et al. Expression of nes?
tin after traumatic brain injury in rat brain〔J〕. Brain Res，
1999，840（1/2）：153-157.
〔13〕LI D，WANG J，YEW D T，et al. Age-related alterations
of Nestin-immunoreactive neurons in rat basal forebrain
with aged memory deficit〔J〕. Neurochem Int，2008，53
（6/8）：270-277.
〔14〕SHAUGHNESSY L W，MUNDY W R，TILSON H A，et al.
Time course of changes in cholinergic and neurotrophinrelated
markers after infusion of colchicine into the basal
forebrain〔J〕. Brain Res，1998，781（1/2）：62-77.
〔15〕周立兵，阮奕文，姚志彬. 秋水仙素对大鼠基底前脑
Nestin 阳性神经元的影响〔J〕. 解剖科学进展，2003，9
（2）：150-108.
〔16〕ZHAO Y，GUO K，LI D，et al. Special function of nestin（+）
neurons in the medial septum-diagonal band of Broca in
adult rats〔J〕. Neural Regen Res，2014，9（3）：308-317.
〔17〕朱丹青，任新，王戚君，等. 基底前脑巢蛋白免疫反应阳性
神经元的来源探索〔J〕. 解剖学研究，2014，36（1）：8-12.