›› 2017, Vol. 2 ›› Issue (2): 40-45.

• 基础医学 • 上一篇    下一篇

hMTERF3 基因启动子区的生物信息学分析

  

  1. (1. 大理大学基础医学院,云南大理671000;2. 云南省昆虫医药研发重点实验室,云南大理671000;
    3. 大理大学大理教学医院呼吸内科,云南大理671000)
  • 收稿日期:2016-01-03 修回日期:2016-05-07 出版日期:2017-02-15 发布日期:2017-02-15
  • 作者简介:孙美涛,硕士研究生,主要从事病理与病理生理学研究.
  • 基金资助:

    国家自然科学基金资助项目(81560458);云南省教育厅科学研究基金资助项目(2014Z126);大理大学博士科
    研启动基金资助项目(BSKY2012018);大理大学大学生创新创业计划资助项目(201402)

Bioinformatics Analysis of hMTERF3 Gene Promoter Region

  1. (1. Pre-clinical College, Dali University, Dali, Yunnan 671000, China; 2. Key Laboratory of Pharmaceutical R & D of Insects in
    Yunnan Province, Dali, Yunnan 671000, China; 3. Respiratory Department, Dali Teaching Hospital of Dali University, Dali,
    Yunnan 671000, China)
  • Received:2016-01-03 Revised:2016-05-07 Online:2017-02-15 Published:2017-02-15

摘要:

目的:探讨人线粒体转录终止因子3基因启动子区的序列特征、转录因子及其结合位点。方法:利用Promoter 2.0、
NNPP、Proscan、FirstEF软件分别预测hMTERF3 基因5端上游的启动子数目及分布;利用CpG Island Searcher和CpG Plot 软
件预测CpG岛位置;利用P-match 1.0程序搜索TRANSFAC数据库预测与hMTERF3 基因启动子结合的转录因子及其结合位
点。结果:hMTERF3 基因定位于8q21.2,基因全长22 216 bp,含有11个外显子和10个内含子。hMTERF3 基因上游至5'侧翼
共3 000 bp的核苷酸序列至少存在2个启动子区,其中1 733~2 302 bp之间可能为包含TATA盒的核心启动子区。启动子区序
列中存在1个长为1 145 bp 的CpG岛。hMTERF3 基因启动子区存在1 055个转录因子结合位点,进化足迹法分析其保守的核
心启动子区转录因子结合位点共19个。结论:hMTERF3 基因启动子区的生物信息学分析能够提高基因启动子的研究效率,为
后续实验构建hMTERF3基因启动子表达载体及鉴定启动子功能提供理论依据。

关键词: hMTERF3, 生物信息学, 启动子区, 转录因子, CpG岛

Abstract:

Objective: To investigate the characters, transcription factors and their binding sites of human mitochondrial transcription
termination factor 3(hMTERF3)gene promoter by different bioinformatics tools. Methods: Promoter 2.0, NNPP, Proscan, and FirstEF
softwares were used to analyze the numbers and distributions of hMTERF3 gene promoter; CpG Islander and CpG Plot softwares were
used to analyze the GpG island of hMTERF3 gene promoter; P-match 1.0 protocol and TRANSFAC database were used to analyze the
transcription factors and their binding sites of hMTERF3 gene promoter. Results: hMTERF3 gene was located on 8q21.2. The full
length of hMTERF3 gene was 22 216 bp, consisting of 11 exons and 10 introns. There were at least two promoters in the 5' unconding
region of hMTERF3 gene. The core promoter of hMTERF3 gene was located between 1 733-2 302 bp, containing TATA box. In
hMTERF3 gene promoter region, a 1 145 bp CpG island could be found. In addition, 1 055 transcription factor binding sites were
predicted by P-Match 1.0 protocol, and only 19 transcription factor binding sites were found in conserved core promoter region of
human and mouse homologous MTERF3 genes by phylogenetic foot- printing analysis. Conclusion: Gene promoter related
bioinformatics analysis can improve the efficiency of hMTERF3 gene promoter research, and provide significant information for the
construction of promoter expression vector, also for the further study of promoter function.

Key words: hMTERF3, bioinformatics, promoter, transcription factor, CpG island

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