›› 2017, Vol. 2 ›› Issue (4): 32-35.

• 药学 • 上一篇    下一篇

云南松松针中黄酮类化合物抗氧化活性研究

  

  1. (1. 大理大学药学与化学学院,云南大理671000;2. 云南省昆虫医药研发重点实验室,云南大理671000)
  • 收稿日期:2016-06-12 修回日期:2016-09-13 出版日期:2017-04-15 发布日期:2017-04-15
  • 作者简介:付文鹏,2013级药学专业本科生.
  • 基金资助:

    大理大学大学生科研基金资助项目(KYSX2015105);大理大学大学生创新创业训练计划资助项目(CXCY201616)

Antioxidant Effect of Flavonoids Compounds from Pine Needles of Pinus yunnanensis Franch

  1. (1. College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China; 2. Key Laboratory of Pharmaceutical R & D of
    Insects in Yunnan Province, Dali, Yunnan 671000, China)
  • Received:2016-06-12 Revised:2016-09-13 Online:2017-04-15 Published:2017-04-15

摘要:

目的:研究云南松松针中黄酮类化合物的抗氧化活性,为云南松松针的开发利用提供依据。方法:用乙醇将干燥、粉碎
后的云南松松针进行提取、精制、冷冻干燥,得总黄酮提取物;用DPPH法研究云南松松针黄酮类化合物清除自由基的能力,用
硫代巴比妥酸法研究云南松松针黄酮类化合物对大鼠肝匀浆自发性脂质过氧化产物丙二醛(MDA)的抑制作用。结果:云南
松松针黄酮类化合物对DPPH自由基有很好的清除作用,能抑制肝匀浆自发性脂质过氧化产物MDA的产生。结论:云南松松
针中黄酮类化合物具有较强的体外抗氧化作用。

关键词: 云南松松针, 黄酮类化合物, DPPH自由基, 抗氧化

Abstract:

Objective: To study the antioxidant activity of flavonoids compounds from pine needles of Pinus yunnanensis Franch and
to provide the basis for the utilization of pine needles of Pinus yunnanensis Franch. Methods: Extraction, purification and freezedrying
treatments were carried out with ethyl alcohol for dried and grinded pine needles of Pinus yunnanensis Franch; thus total
flavonoid extracts were obtained. DPPH was used to study the ability of scavenging free radicals of flavonoids from pine needles of
Pinus yunnanensis Franch. TBA was used to study the inhibiting effect of flavonoids from pine needles of Pinus yunnanensis Franch on
MDA in the mice liver tissue homogenate. Results: Flavonoids from pine needles of Pinus yunnanensis Franch had scavenging effect
on DPPH and could inhibit the generation of spontaneous liver homogenate lipid peroxidation MDA. Conclusion: The flavonoids from
pine needles of Pinus yunnanensis Franch had a strong anti-oxidant effect.

Key words: pine needles of Pinus yunnanensis Franch, flavonoids, DPPH, antioxidation

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