云南泡核桃壳提取物体外抗肝纤维化活性研究

›› 2018, Vol. 3 ›› Issue (4): 5-11.

云南泡核桃壳提取物体外抗肝纤维化活性研究

（1.大理大学药学与化学学院，云南大理671000；2.大理大学第一附属医院神经内科，云南大理671000）

收稿日期:2016-10-12 修回日期:2016-11-17 出版日期:2018-04-15 发布日期:2018-04-15
通讯作者: 李丹丹，主治医师.
作者简介:李春艳，讲师，主要从事天然来源植物药效学研究.
基金资助:
云南省教育厅科学研究基金资助项目（2014Y407）；大理白族自治州科学技术局资助项目（D2017SA03）

A Study of Anti-hepatic Fibrosis Activities of Extracts from Walnut Shell of Juglans sigillata D. in Vitro

（1. College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China; 2. Department of Neurology, The First Affiliated
Hospital of Dali University, Dali, Yunnan 671000, China）

Received:2016-10-12 Revised:2016-11-17 Online:2018-04-15 Published:2018-04-15

摘要/Abstract

摘要：

目的：研究云南泡核桃壳提取物（HTK-D、HTK-F、HTK-DEF）体外抗肝纤维化作用。方法：采用MTT法，观察云南泡核桃壳提取物对HSC增殖的影响及对L-02细胞的毒性作用。ELISA法检测细胞培养上清中TGF-β1、ColⅠ、Col Ⅲ的含量。结果：48、72 h时，HTK-D、HTK-DEF在15.63~1 000.00 μg/mL抑制HSC增殖（P＜0.05），HTK-F对HSC增殖抑制的浓度范围为62.5~1 000.00 μg/mL（P＜0.05）。HTK-D、HTK-F、HTK-DEF分别与L-02细胞作用48 h后，其CC50分别为＞1 000.00、354.73、457.09 μg/mL。HTK-D、HTK-F、HTK-DEF在62.50~1 000.00 μg/mL时均能降低TGF-β1的生成（P＜0.05），且呈浓度依赖性；HTK-D、HTK-F、HTK-DEF分别在各浓度梯度下均能减少Col I、Col Ⅲ的生成（P＜0.05）。结论：云南泡核桃壳提取物具有体外抗肝纤维化作用，其作用机制可能与抑制肝星状细胞增殖，减少TGF-β1、Col I、Col Ⅲ的生成有关。

关键词: 云南漾濞泡核桃壳, 提取物, 抗肝纤维化, 转化生成因子β1, I型胶原, Ⅲ型胶原

Abstract:

Objective: To study the anti-hepatic fibrosis effects of extracts from walnut shell of Juglans sigillata D. in vitro（HTK-D, HTK-F, HTK-DEF）. Methods: The MTT assay was used to observe the effects of the extracts from walnut shell of Juglans sigillata D. on hepatic stellate cells'（HSC）propagation and their cytotoxicity on normal human liver cell（L-02）. ELISA method was used to detect the contents of TGF-β1, collagen Ⅰ and collagen Ⅲ in the supernatant of HSC. Results: Both HTK-D and HTK -DEF（the concentration from 15.63 μg/mL to 1 000.00 μg/mL）had inhibiting effects on HSC after 48 hours and 72 hours（P＜0.05）. HTK-F（62.50 μg/mL-1 000.00 μg/mL）could inhibit the proliferation of HSC for 48,72 h（P＜0.05）. The values of CC50 were >1 000.00 μg/mL, 354.73 μg/mL, 457.09 μg/mL accordingly after L-02 was cultured with HTK-D, HTK-F and HTK-DEF respectively for 48 h. HTK-D, HTK-F and HTK-DEF could reduce the production of TGF-β1 in supernatant for 48 h at concentration between 62.50 μg/mL and 1 000.00 μg/mL in a concentration dependent manner（P＜0.05）. HTK-D, HTK-F and HTK-DEF could decrease the production of collagen Ⅰand collagen Ⅲ in supernatant at different concentration gradient（P＜0.05）. Conclusion: Extracts from walnut shell of Juglans sigillata D. in vitro have definite effects on anti- hepatic fibrosis． The mechanism may be related to inhibiting HSC propagation and reducing the production of TGF-β1, collagen Ⅰ and collagen Ⅲ.

Key words: walnut shell of Juglans sigillata D., extracts , anti-hepatic fibrosis, transforming growth factor-β1, collagen type Ⅰ, collage type Ⅲ

中图分类号:

R965

李春艳, 陈进汝, 董寿堂, 刘熙, 郝芳芳, 李冬梅, 李丹丹. 云南泡核桃壳提取物体外抗肝纤维化活性研究[J]. , 2018, 3(4): 5-11.

Li-Chun-Yan, CHEN Jin-Ru, DONG Shou-Tang, LIU Xi, HAO Fang-Fang, LI Dong-Mei, LI Dan-Dan. A Study of Anti-hepatic Fibrosis Activities of Extracts from Walnut Shell of Juglans sigillata D. in Vitro[J]. , 2018, 3(4): 5-11.

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