载荷利福平和异烟肼的BCP 人工骨在家兔体内释药性能初步研究

doi:10. 3969 / j. issn. 2096-2266. 2021. 08. 002

大理大学学报 ›› 2021, Vol. 6 ›› Issue (8): 7-10.DOI: 10. 3969 / j. issn. 2096-2266. 2021. 08. 002

载荷利福平和异烟肼的BCP 人工骨在家兔体内释药性能初步研究

李忠杰1，刘飞飞1，阮玉山1，丁凯2，雷延发2，和英3，李绍波2*

（1. 大理大学第一附属医院脊柱外科，云南大理 671000；2. 大理大学临床医学院，云南大理
671000；3. 大理大学药学院，云南大理 671000）

收稿日期:2020-05-06 修回日期:2020-12-10 出版日期:2021-08-15 发布日期:2021-09-28
通讯作者: 李绍波，教授，E-mail：lishaobo74@126.com。
作者简介:李忠杰，住院医师，主要从事骨科学基础与临床研究。
基金资助:
云南省教育厅科学研究基金项目（2019Y0297）

Preliminary Study on Drug Release through BCP Artificial Bones Loaded with#br# Rifampicin and Isoniazid in Rabbits

Li Zhongjie1, Liu Feifei1, Ruan Yushan1, Ding Kai2, Lei Yanfa2, He Ying3, Li Shaobo2*

(1. Department of Spinal Surgery, The First Affiliated Hospital of Dali University, Dali, Yunnan 671000, China; 2. Clinical College,
Dali University, Dali, Yunnan 671000, China; 3. College of Pharmacy, Dali University, Dali, Yunnan 671000, China)

Received:2020-05-06 Revised:2020-12-10 Online:2021-08-15 Published:2021-09-28

摘要/Abstract

摘要： 目的：观察载荷利福平和异烟肼的双相磷酸钙（BCP）人工骨在家兔体内药物的释放特点。方法：避光条件下将BCP
人工骨浸入利福平和异烟肼药液中，干燥后获得载药人工骨，用紫外-可见分光光度直接测定法分别测定利福平和异烟肼两药
的载药率。建立家兔髂骨骨缺损模型，空白组（BCP 人工骨组）6 只，实验组18 只（载荷利福平、异烟肼BCP 人工骨组），
术后第1、3、7、14、28、56 天分别取材，测定血浆和局部组织药物浓度。结果：BCP 人工骨利福平载药率为（5.98 ± 0.80）%，
异烟肼载药率为（15.08 ± 2.22）%。同一时间点局部组织及血浆两种样本中药物浓度存在差异，同一时间点局部组织中的药物浓度
高于血浆中的药物浓度，差异有统计学意义（P < 0.05）。结论：载药BCP 人工骨具有抗结核药物缓释效果，能作为后续抗结
核药物缓释相关研究的载体。

关键词: 利福平, 异烟肼, 载药, 双相磷酸钙人工骨

Abstract: Objective: To observe the features of drug release through bidirectional calcium phosphate (BCP) artificial bones loaded
with rifampicin (RFP) and isoniazid (INH) in rabbits. Methods: BCP artificial bones were immersed in rifampicin and isoniazid
solutions under dark conditions, and then dried to obtain drug-loaded artificial bones. Direct ultraviolet-visible spectrophotometry was
used to determine the loading rate of rifampicin and isoniazid respectively. A rabbit iliac bone defect model was established, with 6 in
the blank group (BCP artificial bone group) and 18 in the experimental group (loaded with rifampicin and isoniazid BCP artificial bone
group). Samples were taken on the 1st, 3rd, 7th, 14th, 28th and 56th days after the surgery, and the plasma and local tissue drug concentrations
were determined. Results: The drug loading rate of rifampicin by BCP artificial bone was (5.98±0.80) %, and the drug loading rate of
isoniazid was (15.08±2.22) %. There was a statistical difference in drug concentrations between local tissue and plasma samples at the
same time point, with the drug concentration in local tissue significantly higher than that in plasma (P<0.05). Conclusion: Drug-loaded
BCP artificial bones have a slow-release effect of anti-tuberculosis drugs and can be used as a carrier for subsequent studies on the
slow-release of anti-tuberculosis drugs.

Key words: rifampicin, isoniazid, drug-loaded, BCP artificial bone

中图分类号:

R681.2

李忠杰, 刘飞飞, 阮玉, 丁凯, 雷延发, 和英, 李绍波. 载荷利福平和异烟肼的BCP 人工骨在家兔体内释药性能初步研究[J]. 大理大学学报, 2021, 6(8): 7-10.

Li Zhongjie, Liu Feifei, Ruan Yushan, Ding Kai, Lei Yanfa, He Ying, Li Shaobo. Preliminary Study on Drug Release through BCP Artificial Bones Loaded with#br# Rifampicin and Isoniazid in Rabbits[J]. Journal of Dali University, 2021, 6(8): 7-10.

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载荷利福平和异烟肼的BCP 人工骨在家兔体内释药性能初步研究

Preliminary Study on Drug Release through BCP Artificial Bones Loaded with#br# Rifampicin and Isoniazid in Rabbits

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