鸟分枝杆菌MAV_2927基因编码蛋白质的生物信息学分析

大理大学学报 ›› 2022, Vol. 7 ›› Issue (8): 41-45.

鸟分枝杆菌MAV_2927基因编码蛋白质的生物信息学分析

李祥芳，丁寿鹏，高婧华，吴利先^*

（大理大学基础医学院，云南大理　671000）

收稿日期:2021-10-14 修回日期:2021-11-11 出版日期:2022-08-15 发布日期:2022-09-16
通讯作者: 吴利先，教授，博士，E-mail：w_lixian@163.com。
作者简介:李祥芳，硕士研究生，主要从事非结核分枝杆菌致病机制研究。
基金资助:
国家自然科学基金项目（81760357）

Bioinformatics Analysis of the Protein Encoded by the MAV_2927 Gene of Mycobacterium avium

Li Xiangfang，Ding Shoupeng，Gao Jinghua，Wu Lixian^*

（Pre-clinical College，Dali University，Dali，Yunnan 671000，China）

Received:2021-10-14 Revised:2021-11-11 Online:2022-08-15 Published:2022-09-16

摘要/Abstract

摘要： 目的：用生物信息学分析鸟分枝杆菌（Mycobacterium avium，MAV）致病基因MAV_2927编码的蛋白质。方法：从NCBI数据库获取MAV_2927基因编码蛋白质的基因、氨基酸序列；ProtＰaram tool、SOPMA、SWISS-MODEL软件分析蛋白质的理化性质及二、三级结构；PortScale、SignalP 4.0 Server、TMHMM Server 2.0软件分析蛋白质的亲疏水性、信号肽、跨膜区。结果：MAV_2927基因编码蛋白质含43个氨基酸，相对分子量4.213 kDa，等电点4.72，分子式C183H284N48Ｏ62S2。结论：MAV_2927编码蛋白质含多个T细胞、B细胞抗原表位，表明其可能是感染过程中与宿主相互作用有关的潜在致病因子，可为研究MAC的致病性和临床治疗提供基础。

关键词: 鸟分枝杆菌, MAV_2927, 生物信息学分析, 致病机制

Abstract: Objective：Ｔo analyze the protein encoded by the pathogenic gene MAV_2927 of Mycobacterium avium（MAV） by using bioinformatics. Methods： The gene and amino acid sequence of the protein encoded by the MAV_2927 gene were obtained from NCBI；Protparam tool， SOPMA， SWISS-MODEL were applied to analyze the physical and chemical properties and secondary and tertiary structure of proteins；PortScale， SignalIP 4.0 Server， TMHMM Server 2.0 were used to analyze hydrophilicity and hydrophobicity， signal peptide， and transmembrane region. Results：The protein encoded by the MAV_2927 gene contains 43 amino acids， has a relative molecular weight of 4.213 kDa， an isoelectric point of 4.72， and a molecular formula of C183H284N48O62S2. Conclusion：The protein encoded by MAV_2927 contains multiple T and B cell epitopes， indicating that it may be a potential pathogenic factor related to the host interaction during the infection process， and can provide a basis for studying the pathogenicity of MAC and clinical treatment.

Key words: Mycobacterium avium, MAV_2927, bioinformatics analysis, pathogenesis

中图分类号:

R378.911

李祥芳, 丁寿鹏, 高婧华, 吴利先.

鸟分枝杆菌MAV_2927基因编码蛋白质的生物信息学分析 [J]. 大理大学学报, 2022, 7(8): 41-45.

Li Xiangfang, Ding Shoupeng, Gao Jinghua, Wu Lixian.

Bioinformatics Analysis of the Protein Encoded by the MAV_2927 Gene of Mycobacterium avium [J]. Journal of Dali University, 2022, 7(8): 41-45.

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