大理大学学报 ›› 2023, Vol. 8 ›› Issue (2): 1-7.

• 药学 •    下一篇

三七总皂苷对ox-LDL诱导血小板凋亡的保护作用及机制研究

马永洁1,李玮琪1,伍春婷1,黄新惠1,周昕榆1,牙甫礼12*   

  1. 1.大理大学公共卫生学院,云南大理 6710002.大理大学代谢性疾病转化医学研究院,云南大理 671000

  • 收稿日期:2022-10-13 修回日期:2022-11-16 出版日期:2023-02-15 发布日期:2023-03-02
  • 通讯作者: 牙甫礼,讲师,博士,E-mail:yafuli@yeah.net。
  • 作者简介:马永洁,硕士研究生,主要从事营养与慢性病防治研究。
  • 基金资助:
    云南省基础研究计划项目(202101AT070033);大理大学高层次人才科研启动基金项目(KYBS2021015

The Protective Effect and Mechanism of Panax notoginseng Saponins on ox-LDL-Induced Platelet Apoptosis

Ma Yongjie1 Li Weiqi1 Wu Chunting1 Huang Xinhui1 Zhou Xinyu1 Ya Fuli1 2*   

  1. 1.College of Public Health Dali University Dali Yunnan 671000 China 2. Institute of Translational Medicine for Metabolic Diseases Dali University Dali Yunnan 671000 China

  • Received:2022-10-13 Revised:2022-11-16 Online:2023-02-15 Published:2023-03-02

摘要:

目的:探讨三七总皂苷(PNS)对体外实验中氧化型低密度脂蛋白(ox-LDL)诱导的血小板凋亡的抑制作用及潜在的分子机制。方法:将健康人纯化血小板分别与不同浓度的PNS 110100 μg/mL 共同孵育40 min,然后用50 μg/mL ox-LDL 刺激血小板 60 min后,用流式细胞术检测血小板凋亡的指标包括血小板线粒体膜电位 (Δψm 水平和磷脂酰丝氨酸 PS 的暴露水平;用活性氧 ROS 检测试剂盒测定胞内总ROS水平和超氧化物水平;用蛋白质印迹法检测血小板SrcSyk蛋白的磷酸化水平以及Caspase-3Caspase-9活化水平。结果:与对照组相比,PNS各剂量组显著降低ox-LDL诱导的血小板Δψm去极化(P<0.01),PNS中、高剂量组能抑制ox-LDL诱导的血小板表面PS的暴露水平(P<0.05)、总ROS水平和超氧化物水平(P<0.05)、Caspase-3Caspase-9的活化水平(P<0.05)以及SrcSyk蛋白的磷酸化水平(P<0.05)。结论:PNS可能通过抑制Src/Syk/ROS信号通路发挥抗ox-LDL诱导血小板凋亡的作用。

关键词: font-family:宋体, ">三七总皂苷;氧化型低密度脂蛋白;血小板;凋亡;活性氧

Abstract: Objective To explore the effect of Panax notoginseng saponinsPNS on oxidized low-density lipoproteinox-LDL-induced platelet apoptosis and its potential molecular mechanism in vitro. Methods: Human gel-filtered platelets were pre-treated with different concentrations of PNS 0110100 μg/mL for 40 minutes and then stimulated with 50 μg/mL ox-LDL for 60 minutes. Subsequently indicators of platelet apoptosis including platelet mitochondrial membrane potential(Δψm levels and phosphatidylserinePS exposure levels were detected by flow cytometry. Reactive oxygen speciesROS levels and superoxide levels were measured by commercial ROS assay kit. The phosphorylation levels of Src and Syk proteins as well as the activation levels of Caspase-3 and Caspase-9 were detected by Western blotting. Results: Compared with the control group ox-LDL-induced depolarization of platelets Δψm was significantly reduced in each dose group of PNSP<0.01), ox-LDL-induced exposure level of platelet surface PSP<0.05), total ROS levels and superoxide levelsP<0.05), Caspase-3 and Caspase-9 activation levelsP<0.05),and phosphorylation levels of Src and Syk proteinsP<0.05 were inhibited in the medium and highdose PNS groups. ConclusionPNS may play the role of anti ox-LDL-induced platelet apoptosis by inhibiting Src/Syk/ROS signalling pathway.

Key words: font-family:Times New Roman, ">Panax notoginseng saponinsfont-family:Times New Roman, ">;font-family:Times New Roman, "> oxidized low-density lipoproteinfont-family:Times New Roman, ">;font-family:Times New Roman, "> plateletfont-family:Times New Roman, ">;font-family:Times New Roman, "> apoptosisfont-family:Times New Roman, ">;font-family:Times New Roman, "> reactive oxygen species

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