间歇和有氧运动对大鼠心肌细胞凋亡和NF-κB表达的影响

doi:10. 3969 / j. issn. 2096-2266. 2020. 12. 010

摘要/Abstract

摘要： 目的：探讨间歇和有氧运动对大鼠心肌细胞凋亡和NF-κBp65表达的影响及NF-κBp65在心肌细胞凋亡中的作用。
方法：SD雄性大鼠30只随机分为对照组、有氧运动组和间歇运动组，每组10只，对照组大鼠不运动，间歇和有氧运动组大鼠分
别进行8周间歇和有氧跑台运动。训练结束后，天狼猩红染色观察心脏结构，心电图和血流动力学检测心功能，免疫组化检测
心肌Bax、Bcl-2、p53和NF-κBp65的表达。结果：与对照组比较，间歇和有氧运动大鼠心系数、T波电压、LVSP和±dp/dtmax
均显著升高，LVEDP 和心率显著降低，心肌Bcl-2的MOD 显著升高，Bax、p53、NF-κBp65的MOD 均显著降低，Bcl-2/Bax比
值显著升高。结论：间歇运动和有氧运动均通过上调Bcl-2的表达、下调Bax和p53的表达、提升Bcl-2/Bax比值显著抑制大鼠
心肌细胞凋亡，提升心脏功能，且间歇运动效应优于有氧运动；间歇运动和有氧运动可能通过降低NF-κB的活性上调Bcl-2的
表达，下调p53的表达，抑制心肌细胞凋亡。

关键词: 间歇运动, 有氧运动, NF-κB, 细胞凋亡

Abstract: Objective: To explore the effects of intermittent and aerobic exercise on apoptosis and NF-κBp65 expression in cardiomyocyte of rats and the role of NF-κBp65 in apoptosis. Methods: Thirty male SD rats were randomly divided into a control group, an aerobic group and an intermittent group, with ten in each group. The rats in the control group did not do any exercise, and the rats in the intermittent and aerobic exercise groups were subjected to 8-week intermittent and aerobic treadmill exercises respectively. After the training, their cardiac structures were observed by scarlet staining, the electrocardiogram and hemodynamic indicators were used to detect cardiac functions, and the expressions of Bax, Bcl-2, p53 and NF-κBp65 were detected by immunohistochemistry. Results: Compared with control group, the heart coefficient, T wave voltage, LVSP and ±dp/dtmax increased significantly, LVEDP and
heart rate decreased significantly, the MOD of the Bcl-2 significantly increased, and the MOD of Bax, p53, NF-κBp65 decreased
significantly, the ratio of Bcl-2/Bax increased significantly of the rats in the intermittent and aerobic exercise groups. Conclusion:
Both intermittent exercise and aerobic exercise can significantly inhibit the apoptosis of rat cardiomyocytes by up-regulating the
expression of Bcl-2, down-regulating the expression of Bax and p53 and enhancing the ratio of Bcl-2/Bax, thus improving the cardiac
function. Besides, the effect of intermittent exercise was better than that of aerobic exercise. Intermittent and aerobic exercises may upregulate Bcl-2 expression, down-regulate p53 expression, and inhibit cardiomyocyte apoptosis by reducing NF-κB activity.

Key words: intermittent exercise, aerobic exercise, NF-κB, apoptosis

中图分类号:

G804.2

邢维新. 间歇和有氧运动对大鼠心肌细胞凋亡和NF-κB表达的影响[J]. 大理大学学报, 2020, 5(12): 55-61.

Xing Weixin. Effect of Intermittent and Aerobic Exercise on Apoptosis and NF-κB Expression of#br# Cardiomyocytes in Rats#br#[J]. Journal of Dali University, 2020, 5(12): 55-61.

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