J4 ›› 2013, Vol. 12 ›› Issue (10): 50-52.

• 生命科学 • 上一篇    下一篇

污水中解有机磷细菌的筛选及解磷能力测定

  

  1. 1. 大理学院公共卫生学院,云南大理 671000; 2. 大理学院基础医学院,云南大理 671000
  • 收稿日期:2013-07-01 修回日期:2013-08-27 出版日期:2013-10-15 发布日期:2013-10-15
  • 作者简介:郭乐,在读研究生,主要从事环境流行病学研究.
  • 基金资助:

    国家科技重大专项(ZX07105-004-001-002);
    大理学院洱海保护研究专项课题(KYRH201005)

Screening and Determination Degradation Ability of Organic Phosphate-solubilizing Bacteria in Sewage

  1. 1. College of Public Health, Dali University, Dali, Yunnan 671000, China;
    2. Pre-clinical College, Dali University, Dali, Yunnan 671000, China
  • Received:2013-07-01 Revised:2013-08-27 Online:2013-10-15 Published:2013-10-15

摘要:

采集生活污水,从中分离出具有解有机磷能力的菌株,纯化得到14株解有机磷细菌。通过平板法测定溶磷圈后,筛选
出4株解磷能力较强的菌株进一步做解磷能力的定量测定。结果发现,OPB8、OPB17、OPB15、OPB19菌株达到解磷峰值时,解磷率分别为107.9%、135.1%、31.8%和249.2%,随着培养时间的延长,培养液中的水溶性磷含量均有下降,其中OPB15、OPB19菌株的培养液中的水溶性磷含量分别降低了72.2% 和95.7%。

关键词: 污水, 解有机磷细菌, 筛选, 解磷能力, 测定

Abstract:

Organophosphorus solid medium was adopted to isolate strains that were capable of solving organophosphorus from sewage.14 strains of organic phosphate-solubilizing bacteria were obtained by purification. The dissolved phosphorus transparency circle could be determined through plate method, and 4 phosphate-solubilizing bacteria were selected to determine quantitative degradation ability by plate method. Phosphate-solubilizing peaking rate of OPB8, OPB17, OPB15, OPB19 strain were 107.9%, 135.1%, 31.8% and 249.2%. Then, the water-soluble phosphorus concentration dropped in the nutrient solution, of which OPB15, OPB19 strain of water-soluble phosphorus concentration in the nutrient solution dropped by 72.2% and 72.2% respectively.

Key words: sewage, organic phosphate-solubilizing bacteria, screening, degradation ability, determination

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