The Function of FIBCD1 in Proliferation of Human Lung Cancer Cell Line A549

doi:10. 3969 / j. issn. 2096-2266. 2025. 08. 007

Abstract

Abstract: Objective: To explore the role of fibrinogen-related C domain 1 （FIBCD1） in lung cancer. Methods: A FIBCD1-
knockdown A549 lung cancer cell line was constructed using lentivirus-mediated RNA interference. The knockdown efficiency was
validated by quantitative real-time PCR and Western blotting. Cell proliferation was assessed by MTT assay, while cell cycle
distribution and apoptosis were analyzed via flow cytometry. The effects of FIBCD1 knockdown on colony formation and tumor growth
were evaluated using colony formation assay and in vivo xenograft tumor formation assay in nude mice. Results: FIBCD1 knockdown
significantly inhibited A549 cell proliferation, induced G0/G1 phase cell cycle arrest, and markedly increased the apoptotic rate.
Colony formation assay further confirmed that FIBCD1 suppression impaired the colony-forming ability of A549 cells. In vivo
experiments showed that FIBCD1 knockdown significantly attenuated tumor growth in nude mice, with reduced tumor weight and in
vivo luminescence intensity compared to the control group. Conclusion: FIBCD1 exerts a role in promoting proliferation and inhibiting
apoptosis in lung cancer cells. Its high expression may be closely associated with tumor growth. Suppression of FIBCD1 significantly
reduces the tumorigenic potential of lung cancer cells, indicating that FIBCD1 holds promise as a potential therapeutic target for lung
cancer.

Key words: fibrinogen-related C domain 1, flow cytometry, colony formation, in vivo xenograft tumor formation assay

CLC Number:

R734.2

Li Hua, Zhang Min, Fang Peifei, Zhang Yanfei. The Function of FIBCD1 in Proliferation of Human Lung Cancer Cell Line A549[J]. Journal of Dali University, 2025, 10(8): 51-56.

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References

〔1〕 JEONG I H，YUN J K，JIN J O，et al. E3 Ligase SOCS3
Regulates NOD2 Expression by Ubiquitin Proteasome Sys⁃
tem in Lung Cancer Progression〔J〕. Cell Oncol （Dordr），
2024，47（3）：819-832.
〔2〕 SANTARPIA M，CIAPPINA G，SPAGNOLO C C，et al.
Targeted Therapies for Kras-Mutant Non-Small Cell Lung
Cancer：From Preclinical Studies to Clinical Developmenta
Narrative Review〔J〕. Transl Lung Cancer Res，2023，12
（2）：346-368.
〔3〕 ZHANG C，ZHOU L J，ZHANG M Y，et al. H3K18 Lacty⁃
lation Potentiates Immune Escape of Non-Small Cell Lung
Cancer〔J〕. Cancer Res，2024，84（21）：3589-3601.
〔4〕 WEST H J，KIM J Y. Rapid Advances in Resectable Non-
Small Cell Lung Cancer：A Narrative Review〔J〕. JAMA
Oncol，2024，10（2）：249-255.
〔5〕 SCHLOSSER A，THOMSEN T，MOELLER J B，et al. Char⁃
acterization of FIBCD1 as an Acetyl Group-Binding Recep⁃
tor That Binds Chitin〔J〕. J Immunol，2009，183（6）：3800-
3809.
〔6〕 JIANG C Y，ZHU J H，ZHOU P C，et al. Overexpression of
FIBCD1 Is Predictive of Poor Prognosis in Gastric Cancer〔J〕.
Am J Clin Pathol，2018，149（6）：474-483.
〔7〕 WANG Y，SUN M J，LIU J B，et al. FIBCD1 Overexpres⁃
sion Predicts Poor Prognosis in Patients with Hepatocellu⁃
lar Carcinoma〔J〕. Oncol Lett，2020，19（1）：795-804.
〔8〕 PFAFFL M W，HORGAN G W，DEMPFLE L. Relative
Expression Software Tool （Rest） for Group-Wise Compari⁃
son and Statistical Analysis of Relative Expression Results
in Real-Time PCR〔J〕. Nucleic Acids Res，2002，30（9）：
e36.
〔9〕 LI L H，HE J，HUA D，et al. Lentivirus-Mediated Inhibi⁃
tion of Med19 Suppresses Growth of Breast Cancer Cells
in vitro〔J〕. Cancer Chemother Pharmacol，2011，68（1）：

207-215.

〔10〕 LOIS C，HONG E J，PEASE S，et al. Germline Transmis⁃
sion and Tissue-Specific Expression of Transgenes Delivered
by Lentiviral Vectors〔J〕. Science，2002，295（5556）：
868-872.
〔11〕赵森，周华. 地衣酸对胰腺癌细胞系PANC-1的抗增殖
作用〔J〕. 大理大学学报，2024，9（10）：26-31.
〔12〕 MILNER A E，LEVENS J M，GREGORY C D. Flow Cyto⁃
metric Methods of Analyzing Apoptotic Cells〔J〕. Methods
Mol Biol，1998，80：347-354.
〔13〕黄山，金礼权，喻宇，等. SOX9在小鼠肝卵圆细胞定向
分化为胆管上皮细胞过程中的表达〔J〕. 大理大学
学报，2023，8（4）：22-27.
〔14〕 KOOPMAN G，REUTELINGSPERGER C P，KUIJTEN
G A，et al. Annexin V for Flow Cytometric Detection of
Phosphatidylserine Expression on B Cells Undergoing
Apoptosis〔J〕. Blood，1994，84（5）：1415-1420.
〔15〕 LIU N，BI F，PAN Y，et al. Reversal of the Malignant
Phenotype of Gastric Cancer Cells by Inhibition of RhoA
Expression and Activity〔J〕. Clin Cancer Res，2004，10
（18 pt 1）：6239-6247.
〔16〕 TOYOSHIMA M，TANAKA Y，MATUMOTO M，et al.
Generation of a Syngeneic Mouse Model to Study the Intra⁃
peritoneal Dissemination of Ovarian Cancer with in vivo
Luciferase Imaging〔J〕. Luminescence，2009，24（5）：324-
331.
〔17〕 NEFF B A，VOSS S G，ALLEN C，et al. Bioluminescent
Imaging of Intracranial Vestibular Schwannoma Xeno⁃
grafts in NOD/SCID Mice〔J〕. Otol Neurotol，2009，30
（1）：105-111.
〔18〕 BUETER C L，SPECHT C A，LEVITZ S M. Innate Sensing
of Chitin and Chitosan〔J〕. PLoS Pathog，2013，9（1）：
e1003080.
〔19〕 VON HUTH S，MOELLER J B，SCHLOSSER A，et al.
Immunohistochemical Localization of Fibrinogen C Domain
Containing 1 on Epithelial and Mucosal Surfaces in Human
Tissues〔J〕. J Histochem Cytochem，2018，66（2）：85-97.
〔20〕 SHAHGOLI V K，DUBIK M，PILECKI B，et al. Expres⁃
sion of FIBCD1 by Intestinal Epithelial Cells Alleviates
Inflammation-Driven Tumorigenesis in a Mouse Model of
Colorectal Cancer〔J〕. Front Oncol，2023，13：1280891.

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[2]	Qiu Youzheng, Wang Jing. Visual Analysis of LncRNA Research in Non-Small Cell Lung Cancer Based on Web of Science [J]. Journal of Dali University, 2024, 9(4): 74-81.
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