J4 ›› 2011, Vol. 10 ›› Issue (12): 97-98.

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Isolation and Identification of Bacteriophages Specific to Intestinal Pathogen in the Water of Downstream of Erhai Lake

Objective: To investigate the distribution of intestinal bacteria in the downstream of Erhai Lake by isolation and
identification phage of intestinal bacteria. Methods: Plaque method was applied to isolate and identify bacteriophages specific to intestinal pathogens in the water of Erhai Lake. Results: Bacteriophages specific to dysentery bacillus, typhoid bacillus, paratyphoid B bacteria, E. coli were successfully isolated. Conclusion: The downstream water of Erhai Lake was contaminated by dysentery bacillus, typhoid bacillus, paratyphoid B bacteria and E. coli. This suggested that it is necessary to further strengthen the management of surrounding living sewage discharge in Dali City.   

  1. School of Basic Medicine, Dali University, Dali, Yunnan 671000, China
  • Received:2011-10-12 Online:2011-12-15 Published:2011-12-15

Abstract:

Objective: To investigate the distribution of intestinal bacteria in the downstream of Erhai Lake by isolation and
identification phage of intestinal bacteria. Methods: Plaque method was applied to isolate and identify bacteriophages specific to intestinal pathogens in the water of Erhai Lake. Results: Bacteriophages specific to dysentery bacillus, typhoid bacillus, paratyphoid B bacteria, E. coli were successfully isolated. Conclusion: The downstream water of Erhai Lake was contaminated by dysentery bacillus, typhoid bacillus, paratyphoid B bacteria and E. coli. This suggested that it is necessary to further strengthen the management of surrounding living sewage discharge in Dali City.

Key words: intestinal pathogen, Erhai Lake, phage

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