Quantitative Analysis of P-phenylenediamine in Natural Hair Dyes

J4 ›› 2011, Vol. 10 ›› Issue (6): 1-3.

Quantitative Analysis of P-phenylenediamine in Natural Hair Dyes

Objective：To detect the content of p-phenylenediamine in five representative kinds of natural black hair dyes in the market and evaluate the quality and safety with industry standards．Methods： p-phenylenediamine was determined by HPLC with a Eclipse XDB-C_l8 column (4.6mm×150mm，5 μm)．Mobile phase Was 5％acetonitrile-bufer solution of phosphoric acid at velocity 0．8 mL/min．UV detection wavelength was 280nm．Results：The linear range of p-pheny1enediamine was between 0．025 0 and 0．400 0 mg/mL(r=0．999 9)．The average recovery was 99．27％with RSD 1．49％ (n=6)．The content of p-phenylenediamine varied from 0．23％ to 1．87％ in five kinds of hair dyes．Conclusion：P-phenylenediamine was detected in five hair dyes，but none exceeded the limited standard．This method is accurate，sensitive and reproducible and may be applied to detect the content of p-phenylenediamine in the natural hair dyes．

Received:2011-04-13 Online:2011-06-01 Published:2011-06-01

Abstract

Abstract:

Key words: jnatural hair dyes, p-phenylenediamine, content determination, PLC

CLC Number:

TQ617．6

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Quantitative Analysis of P-phenylenediamine in Natural Hair Dyes

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