Table of Content

01 June 2011, Volume 10 Issue 6

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Quantitative Analysis of P-phenylenediamine in Natural Hair Dyes

2011, 10(6):  1-3. 

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Objective：To detect the content of p-phenylenediamine in five representative kinds of natural black hair dyes in the market and evaluate the quality and safety with industry standards．Methods： p-phenylenediamine was determined by HPLC with a Eclipse XDB-C_l8 column (4.6mm×150mm，5 μm)．Mobile phase Was 5％acetonitrile-bufer solution of phosphoric acid at velocity 0．8 mL/min．UV detection wavelength was 280nm．Results：The linear range of p-pheny1enediamine was between 0．025 0 and 0．400 0 mg/mL(r=0．999 9)．The average recovery was 99．27％with RSD 1．49％ (n=6)．The content of p-phenylenediamine varied from 0．23％ to 1．87％ in five kinds of hair dyes．Conclusion：P-phenylenediamine was detected in five hair dyes，but none exceeded the limited standard．This method is accurate，sensitive and reproducible and may be applied to detect the content of  p-phenylenediamine in the natural hair dyes．

Determination of Gatifloxaein Level in Urine with Ultraviolet Spectrophotometry

2011, 10(6):  4-6. 

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Objective：To determine the content of Gatifloxacin in urine with ultraviolet spe(：trophotometry method．Methods：Urine samples from 5 healthy volunteers were collected．The contents of Gatifloxacin in unne were determined by ultraviolet speetrophotometry with the detective wavelength of 333 nm．Results：Data have shown a good linear relation between Gatifloxacin eotent and absorbance (r=0．999 8) with the Gatifloxacin concentration in 1．00 to 36．00 μg/mL．The average recovery was 98．1％with RSD 2．87％ ; the average
extraction recovery was 95．4％ with RSD 3．66％ ，excretion rate in 24 hours was 443％～69．6％ . Conclusion：The simple，quick，sensitive and accurate method applied here is worthy of promoting in Gatifloxacin determine in urine．

Absorption and Desorption Efect of M acroporous Resin on P0lysaccharide from Periplaneta Americana

2011, 10(6):  7-10. 

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Objective：To select the optimal macroporous absorbing resin for Periplaneta americana polysaccharide from absorption and desorption nleasurement in 6 kinds of different Macroporous resin including D-101，HPD-400，AB-8 etc．Methods：Ultraviolet spectrophotometry was employed to determine the absorption of different macroporons absorbing resin to Periplaneta aztlericatla polysaccharide．Results：Among the 6 resin candidates，HPD-400 resin which was with 41．5％ dynamic absorption ratio and 97％ dynamic desorp tion
ratio has shown relatively highest effect on absorption and desorp tion．Conclusion：HPD-400 resin was a preferable candidate for absorbing Periplan eta americana polysaccharide．

Content Determination of Gentiopicr0side in Boyunding

2011, 10(6):  11-13. 

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Objective：To establish a method to determine the content of gentiopicroside in Boyunding．Methods：The content of gentiopierosidewasdeterminedbyhighperformanceliquid chromatography．Agilent TC-C1 8 (4.6 mm ×150 mm，5 μm ) was used as analytical column，the mobile phase was composed of methanol amt warer，the detection wavelength was 274 nm．Results：The calibration curve has shown a good linear pattern in gentiopicroside content within 0.219 2~2.192 g(r=0．999 8)，the average recovery ratewas 97．6％ (n=9)
and RSD was 2．37％ ．Conclusion：The simple，stable and reproducible properties of this method enable its application in content measuration of gentiopicroside in Boyunding．

Inhibitory Efect of Scutellarin-7-o-gluronide on Gastric Cancer Cell Lines in Vitro

2011, 10(6):  14-16. 

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Objective：To study preliminarily the efect of anti-tumor activity of Scutellarin-7-0-g1u l1r0nide on gastric cancel cell lines． Methods：MTT assay was applied to investigate the inhibitmy effect of Scutellarin-7-O-glucuronide on gastric cancer cell SGC-7901 and BGC-823．Results：With Seutellarin-7-0-glucuronide concentration of 1 mg／mL，the inhibitory proportion on SGC-7901 was 47．7％, MTT results showed that there was a dose-dependence and time-dependence manner with Scutellarin-7-0-glucuronide inhibitm3,effect on SGC-7901 cell line．And there was no inhibitory efect observed on BGC-823 cell line with Scutellarin-7-o-glucuronide concentration of 1 mg／mL．Conclusion：Seutellarin-7-o-glucuronide at concentration of 1 mg／mL exhibited anti-tumor activity on SGC-791 gastric cancer cell and on  anti-tumor activity on BGC-823 cell line．

Efect of TGF-β1 and EGF on the Phenotypical Change and Function of Type II Alveolar Epithelial Cells

2011, 10(6):  17-20. 

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Human Cyclin E Cloning and Eukaryotic Expression Vector Construction

2011, 10(6):  21-24. 

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Objective：To construct eukaryotic expression vector with FLAG epitope highly expressing human cyclin E gene．Methods：Amplify the cDNA of cyclin E gene from total RNA isolated from HeLa cells using RT-PCR．After sequenced，the open reading frame(ORF)of cyclin E cDNA was cloned into eukaryotic expression vector p3XFLAG—CMV^TM-14 to form a recombinant plasmid naIned as p3XFLAG- cyclin E．Then lipofectamine 2000 was used to transfected eukaryotic expression vectors into HeLa cells．Finally，Western—blot was applied to detect the expression of human cyclin E in HeLa cells．Results：Restriction enzyme digestion and nucleotide sequencing results confirmed that the recombinant plasmid was successfully constructed．By W estern-blot，we found that the human cyclin E were expressed in HeLa cells．Conclusion： W e cloned human cyclin E and constructed eukaryotic expression vector，which enable US do further research with cyclin proteins．

Methodological Study of Passive Avoidance Test of Learning and Memory Function in M ice

2011, 10(6):  25-27. 

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Objectives：To improve the sensitivity ofpassive avoidance test，three various testing methods were applied to estimate the learning and menlory function in lnice model with dysmnesia induced by scopolamine．M ethods：Scopolamine was used to duplicate dysmnesia models in mice．Latency time and numbers of error were obtained by simple test method，double test method and muhi—test method．The senstitivities of each method were compared．Results：In simple test，there were no signifcant differences between model group and control group in both latency time and numbers of error．While data have not shown significant difference on latency time in double test．However，in multi—test，there were significant diferences in both two indexes eompared to the other two groups．Conclusion：Multi—test was the most sensitive method to nleasure the learn ing and memory ability in animal during passive avoidance test．

Survey of the Indoor Air Bacterial Contamination of a Kindergarten in Dali City

2011, 10(6):  28-30. 

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Objectives：To investigate indoor air bacterial contamination of a kindergarten in Dali，and to explore the its relationship with the incidence of respiratou disease of young children．M ethods：Natural sedimentation method was applied to detect total bacterial count and hemolytic streptococcus of indoor air in 4 classrooms including the classroom of pre—school，lop class，middle class and bottom class in the kindergarten．Tracking survey of respiratory disease on children was conducted in 1 week after sampling and analyzed statistically．Results：The pass rate of total bacterial count was 50％ in air of all classrooms，henmlytic streptococcus was not detected．There is no significant difference in morbidity rates ofrespiratory tract infection among children with different gtades after slalistieal analysis(P>0．05)，and statistical analysis also showed that there is no significant difference between the morbidity rate of respiratory tract infeclion of children andtheIotaI1)acterial count ofindoorair(P>O．05)．Conclusion：Therewere still bacteria eontamination ofindoorairin part ofkindergartens in Dali City，management of air sanitation shonld be improved．

Quality Analysis of Internet—based Infectious Disease Report in Fugong County from 2006 to 2008

2011, 10(6):  31-32. 

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Objectives：To analyze the reporting quMi~of infectious diseases in Fugong County from 2006 to 2008 and provide a scientific method for management of notifiable infectious diseases．M ethods：Data from information management system fro disease surveillance report under “China Information System for Disease Control and Prevention”during 2006 to 2008 were collected and exported in excel format，and the reporting quality was evaluated with different indicators，Results：Timely report failure rates in Fugong County were 49．68％，48．26％ ，and 20．45％ respectively in 2006，2007 and 2008，and timely vefi~ing failure rates were 1 6．24％，7．36％and 2．75％．Conclusion：The timeliness，completeness and accuracy rates of reporting in 2008 were significantly higher compared to that in 2006．The reporting quality of notifiable infectious diseases has been improved．

Survey of Drug Characteristics and AIDS-related ：Knowledge in 339 Cases of M ale Addicts under Coercive Drug Rehabilitation

2011, 10(6):  33-35. 

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Objectives：To explore AIDS—related knowledge and drug eharacteristies among male addicts under coereive drug rehabilitation in Dali Labor Education Station．Methods：By cluster rand—Dill sampling，350 questionnaires of drug addicts in Dali Labor Education Station about demographic characteristics，behaviour and AIDS—related knowledge were analyzed． Results：The number of survey collected was 339．Among the addicts，the youngest was 1 6 years old，and the oldest was 60．the average age was33． 34±7．34．
The edu cational backgrounds of the addicts under coercive drug rehabilitation mainly were elementary school (3 1．6％) and junior high school(51．O％ )．The average length of drug addiction was 8．94-4-6．42 year．with the shortest of 6 months and the longest of 43 years．56.3％ of addicts were simple oral dug，26．8％ of addicts were intravenous；drug with sharing syringes．Compared to TV programs．news—papers an(1 books and other resources，their HIV／AIDS—relates1 knowledge were largely obtained from the train course in Labor Edueati0n Station．76％ of adicts knew how to prevent from H1V／AIDS in ction．Conclusion：It is vital to take comprehensive measures t0 improve HIV／AIDS—related knowledge education in the drug addiction population in order to rednee HIV infection.

Pathogens in Pulmonary Tuberculosis Complicated Low Respiratory Tract Infection：The Distribution and Antimicrobial Resistance Analysis of 489 Strains

2011, 10(6):  36-38. 

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lAbstract]Objective：To investigate the distribution and antimicrobial resistanee of sputum samples from patients with pulmonary tuberculosis combined with lower respiratory tract infection in our hospital to guide a rational use of drugs．Methods：Pathogens isolated from 1 32 1 sputum samples from patients with pulmonary tuberculosis and lower respiratmT tract infection in our hospital in the last two years were analyzed retrospectively．Results：Four hundred and eighty—nine pathogens were detected in 1 32 1 samples, including 1 84 (37．6％ )strains of gram—positive bacteiia，which were mainly Staphylococcus epidermidis，Enterococcus faecalis．Streptococcus and Staphylococcus aureus；1 69 (34．6％ )strains ofgram—negative bacteria，which were mainly Escherichia coli，calcium acetate Acinetobacter．Pseudomouas and Klebsiella；1 36 (27．8％ )strains of fungi．Ⅲost of which were Candida albicans．Drug susceptibility results(except
fungi j showed that most gram—positive and gram-negative bacteria were resistant to a variety of antibiotics．And the antibiotics with higher resis tance rates were：oxacillin，clindamycin，sulfamethoxazole，erythromycin，vaneomycin，penicillin sodium，ciprofloxacin， levofloxacin；relatively higher sensitive antibiotics were imipenem，cefepime，amikacin，tobramycin，cefotaxime sodium ，cefiriaxone
sodium，cefoperazone sodium．Conclusion：Pathogens in puhnonary tuberculosis and bacterial lower respiratory tract infection were mostly opportunistic pathogens，which were with nmhiple dru g resistance．Rational USe of antibiotics based on drug susceptibility results and clinical monitoring should be strengthened．

Clinical Analysis of 17 Cases of Cerebral Ventricle Cysticercosis

2011, 10(6):  39-41. 

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Objective：To investigate the diagnosis and surgical treatment of cerebral ventricle cysticercosis．Methods：A retrospective analysis of 17 patients who were hospitalized in our department from September 2005 to June 2010 with cerebral ventricle cystieercosis was performed．Four cases were lateral ventricles eysticercosis and 9 cases were fourth ventricle cysticercosis，all 1 3 cases were resected by surgery．Two cases of lateral ventricles cysticercosis were treated with ventriculoscope．All 1 5 patients above were treated by dehydration
and antiparasitic measures postoperatively．Other 2 eases were third ventricles cysticercosis and they were treated with conservative antiparasitic measure．Results：All 15 patients treated with operation were successfldly performed，whose symptoms of intracranial hypertension were immediately relieved after operative procedures．There were no nervous lesion newly found for 3-24 months postoperative follow—up．The
conditions of two patients with no symptoms of intracranial hypertension were much improved with consmwative antiparasitic measures．Conclusion：MRI is valuable in diagnosis and treatment of cerebral ventricle cysticercosis．Surgical method is important for the treatment of cerebral venlricle cysticercosis．

Clinical Application of Intradermal and Intermittent Subcutaneous Suture in General Surgical Incisio

2011, 10(6):  42-43. 

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Objective：To discuss the adventages of intermittent subcutaneous and intraderrnal suture in general surgical incision．Methods：Patients in general surgery were applied diferent surgical methods．The conditions of wound healing，scar sizes and rates of infection were compared between the group of intermittent subcutaneous and intradermal suture and traditional suture group．Results：The research data showed there were good wound healing，small scar，no centipede legs—like scars，and low infection rates in intermittenl subcutaneous and intrademlal suture group．Conclusion：Intermittent subcutaneous and intradermal suture method was better compared to the traditional suture in general surgical incision．

Operation of Complex Staghorn Kidney Stones

2011, 10(6):  44-45. 

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Clinical Analysis of 47 Cases of Portal Hypertension Treated with Modified Sugiura Operation

LIU Hong, MAO Yuan-Yuan

2011, 10(6):  46-47. 

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Objecfive：To assess the efects of modified sugiura surgical procedure for portal hypertension．Methods：Clinical documents of 47 patients with portal hypertension treated by modified sugiura operation were analyzed retrospectively．Results：There was good effect of modified sugiura surgical procedure for po~al hype~ension in preyenting recent post-operative bleeding，one patient died，the mortality was 2.01％ (1/47)．The syndrome of esophageal varices disappeared or markedly improved after the operation in the rest patients．Two
patients rebleeded，}he rebleeding rate was 4.4％ (2/47)．No hepatic eneephalopathy，anastomotic leakage，nor stricture was obsmwed．Conclusion：Modified sugiura operation was an effective treatment tbr portal hypertension，with relatively lnore eonvenient operation and less complications．

Investigation into the Reasons of Symptom Recrudescence after Cholecystectomy

WANG Zhi, WU Jia-Jun, BAI Jun-Chao

2011, 10(6):  48-50. 

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Objective：To analyze and investigate the causes of the syndrome recurences with post—cholecystectomy．Methods：124 cases patient ofthe syndrome recurrences with p0sf—ch0lecystect0my from JanuatT 2006 to December 2010 were retrospecti ely reviewed．Results：The caHses of syndrome recurrences after cholecystectomy include Vater bulb cutaneous 46 cases (37．1％ )：f0ssa for gallbladder fluidify 28 cases(22．6％)；stricture of common bile duct 1 2 cases(9．7％)，cystic duct remnant 9 cases(7．3％)and all of two are 6 cases(4．8％ )．The other reasons are stricture of common bile duct 8 cases(6．5％ )and c3 stic duct remnant 9 cases(7．6％ )．chronic pancreatitis and cancer of hepatopancreatic ampulla and SO on are 6 cases (9．7％)．Conclusion：There are many reasons of syndrome recurences with postch0lecystectomy．Reduce the the recurrence rate after cholecystectomy should be conducted related examinalion before operalions，elaborative exploration and cut down rudimental biliary calculi.

Effects of NF-KB and TNF-α on Endometriosis

2011, 10(6):  51-53. 

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 0bjective：The expression of NF-κB in the tissue of endometria and tumor necrosis factor- α in serum in endometriosis patients were measured to investigate the relationship between NF-κB．TNF-α and endometriosis．Methods：Enzyme linked im munosorbent assay method was applied to detect the the expression of TNF-α．The expression of NF-κB was detected by immunohis tochemistry method．Results：The TNF- α serum level of endometriosis patients was significantly higher compared to the normal control(P<0.01)．The expression of NF-κB was major located in epithelioglandnlar kytoplasm of trusion-endometriosis，and the
expression of NF-κB during progestational stage was higher than that in proliferative stage (P<0．05). Conclusion：The abnormal expression of NF-κB and TNF-α were related to endometriosisB in the tissue of endometria and tumor necrosis factor- in serum in endometriosis.

Three-dimensional Transvaginal Ultrasound Fallopian Tube Imaging in Infertility Diagnosis

2011, 10(6):  54-56. 

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0bjective：To investigate the three—dimensional transvaginal ultrasound fallopian tube imaging in the diagnosis and treatment of infertility．Methods：Twenty patients with infertility were conducted the uhrasound fallopian tube imaging and medicine treatment under three—dimensional transvaginal ultrasound surveillance with contrasl agent of SonoVue．The results were compared with those of laparoscopy．Results：Compared with laparoscopic findings，18 cases were correctly diagnosed with 3D-HyCoSy method，the accuracy rate was 90%．Ten patients reached therapeutic purpose，the effective rate was 50％．Conclusion：The application of ultrasound contrast agent SonoVue in three dimensional transvaginal hysterosalpingography was simple，safe，non-invasive，and non-radiate，and highly efficient．This method was helpful for the diagnosis and therapeutic effect of infertility．

Application ＆ Analysis of Non-invasive Painless Technology in the Extraction of Deciduous Teeth

2011, 10(6):  57-58. 

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Objective：To evaluate the efect of surface anesthesia on children’S dental fear in the extraction of deciduous teeth．Methods：200 pre-school children (3-7 years old)and 200 school—age children (7-12 years old)were selected for research．Each age group was randomly divided into the surface anesthesia group and the control group．The surface anesthesia group was conducted with surface anesthesia of 20％ benzocaine gel before the deciduous teeth extraction operation，and the control group was operated without surface anesthesia．Responses of each case were recorded after the operation．Results：Compared with the control group，the rates of dental fear in surface anesthesia group were significantly lower (P<0．05)both in pre-school children and school-age children．Conclusion：Surface anesthesia of benzoeaine gel prevents children from dental fear in the extraetion of deciduous teeth．

Clinical Analysis of the Dry Eye after Pterygium Excision Misdiagnosed as Viral Keratitis

2011, 10(6):  59-60. 

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Objective：To investigate the dry eye prone pathogenesis after pterygium excision and the avoidance of misdiagnosis as viral kerati!is．Methods：The clinical data of 38 eyes from 26 patients were analyzed．Results：All cases were conducted the pterygium excision and were postoperative dry eye misdiagnosed as viral keratitis．After discontinuation of antiviral therapy，all eases were cured through the treatment of dry eye．Conclusion：Clinicians should fully grasp the pathogenesis of dry eye and the diferential diagnosis of dry eye from viral keratitis in order to avoid misdiagnosis and delayed treatment．

Research in Antibiotic Prophy laxis for Type I Incision Operation in Afiliated Hospital of Dali University

2011, 10(6):  61-63. 

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Objective：To investigate the application of antibiotic prophylaxis for type I incision operations in our hospita1．Methods：Seven hundred and forty two cases with type I incision operation in our hospital from January to June in 2010 were conducted a retrospective analysis．Results：All cases of the type I incision patients received prophylactic antibiotic，among which 195 cases were applied with no indication drug，while 209 eases were applied with drug combination．There was much difference in first administration time，1 69 eases(22．8％ )were 0．5-2h preoperative；266 cases(35．8％)were more than 2h preoperative；and 307 cases(41．4％ )were applied postoperatively．Conclusion：UnneeessaEy high-grade antibiotic，inappropriate and prolonged usage，as well as high utilization rate of the drag combination were the main problems in the application of antibiotic prophylaxis for type I incision operations in our hospital，which needed improved management．

A New Target of Peritoneal Ultrafiltration Failure in Peritoneal Dialysis Patients：Peritoneal Angiogenesi

2011, 10(6):  64-67. 

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Objective：To analyze the major causes and tactics of uhrafihration failure in long peritoneal dialysis patients．Methods：Through a large number of documents，we reviewed the characteristics of peritoneal angiogenesis research progress from the current points of incidence of peritoneal uhrafihration failure，and the change of peritoneal structure and funelion．Results：Vm‘ious eauses can stimulate the expression of VEGF in peritoneal membrane tissue，and promote the proliferation of new vascular，which result in increased effective peritoneal surface area of peritoneal membrane and reducd uhrafihration．Peritoneal angiogenesis was the main reason of peritoneal ultrafiltration failure．Conclusion：Peritoneal angiogenesis was the major reason of peritoneal ultrafihration failure，which WflS also the reseamh focus．There were a variety of methods to interfere the formation of new blood vessels．

Effect of Nursing intervention in Adverse Reactions of Fundns Fluorescein Angiography

2011, 10(6):  68-70. 

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Objective：To discuss the effect of nursing intervention in adverse reactions of fimdus fluorescein angiography(FFA)．Methods：Two thousands four hundreds and thirty six patients who conducted FFA from February 2005 to February 2011 in our hospital were divided into group A (nursing intervention group)and group B (non nursing intervention group)．The clinical manifestation and turnover of the adverse reactions in each group were analyzed．ResuIts：The incidence rate of adverse reactions was 6.32％ in group B．and 1.40％ in group A．Conclusion：Effective nursing intmwention can decrease the occurrence of complications during FFA．

Practice and Efect Evaluation of Clinical Nursing Pathway in Gynecological Single Diseas

2011, 10(6):  71-73. 

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Objectives：To investigate the effect of clinical nursing pathway in laparoscopic surgery for gynecological single disease．Methods：Two hundred and thirty four patients of gynecological single disease who had conducted laparoseopie surgery in our department from March 2010 to December 2010 were randondy divided into experimental and control group and 1 1 7 cases in each group．Conventional treatment and health education were applied in control group，while the clinical nursing pathway schedule was strictly conducted in the experimental group．Results：There were significant diferences in the achievement rate of patient health education，compliance behavior and degree of satisfaction between two groups(P<0．05)．Conclusion：The application of clinical nursing pathway was good way in single disease care which could improve the quality of care and patient satisfaction and standardize the care behavior and train young nurses．

Practice and Reflection on High-quality Nursing Service Demonstration Activity

2011, 10(6):  74-76. 

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Objectives：To implement the reform of medical and heahh system and patient—oriented concept，and to standardize clinical nursing，strengthen basic nursing，improve nursing service and ensure patients safety．Methods：Implementation plan of high-quality nursing service demonstration activity was carried out and one high—quality nursing service pilot ward was fixed after deep understanding of the document and well—prepared propaganda work．Results：The nursing quality of the pilot ward was c0mprehensively improved both in
nurse—patient communication and trust and in patient’S and their family’S satisfaction degrees．Conclusion：Good basic nursing work is the breakthrough point of holistic nursing and the cornerstone of improving professional standards，as well as the important channel of medical safety and harmonious doctor-patient relationship．

Experiences in High—quality Nursing Service“Demonstration Project”

2011, 10(6):  77-78. 

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Objective：To promote the initiative of participation in high—quality nursing“demonstration projeel”through varies strategies．Methods：We improved nursing quality by staff training and propaganda and through studies on execution and other related theories．Results：After the demonstration project，a clear connotation of high—quality care and responsibilities were recognized in aU nuring staffs，who showed more increased participation initiative．Conclusion：“Demonstration project”programs and related activities could be implemented by taking various effective measures．

A Brief Analysis of the Quality and Competence for Modern Operating Room Nurse

2011, 10(6):  79-81. 

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Objective：To explore the quality and competence for modern operating room nurses．Methods：From the aspects of professional knowledge，operating skill，psychological quality，emergency—dealing ability，legal consciousness and medical ethics，the qualifications of operating room nurses in operating room work were analyzed．Results：The basic quality and competence of operation room nurses in this special medical environment are important foundations to guarantee the quality of surgery．Conclusions：Skilled professional basis，excellent psychological quality and communication skills，keen observation，outstanding emergency—dealing ability and high legal consciousness are essential for modern operating roonl nurses．Capable nurses can ensure operation smoothly，improve nursing quality of operating patients and reduce care disputes．

First—aid Treatment of Opioid Poisoning Patients with Mannitol

2011, 10(6):  82-83. 

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0bjective：To investigate the effect of Mannitol treatment in opioid poisoning patients．Methods：Forty six patients of opioid intoxication caused by damaged packaging foreign body admitted in our department were enrolled in the researeh．In addition to the regular Naloxone treatment，patients in the research were received twenty percent of Mannitol for purgation．Results：Thirty seven patients evacuated watery stool in two hours，9 cases who did not evacuated a stool after two hours were received 200mL of twenty percent Mannitol again．
All patients began to evacuate foreign body eighteen hours after admission．the longest lime of excreting alI foreign body was 94 hours．Conclusion：It is efective in excretion opioid toxic，dehydration and diuresis with Mannitol treatment for opioid poisoning patients through gastrointestinal tract．

Experiences of PICC Application in Trauma Patients

2011, 10(6):  84-85. 

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0bjective：To investigate the efects of peripherally inserted central catheter (PICC)treatment in early trauma patients．Methods：Retrospective analysis was conducted in 1 08 cases of trauma patients with PICC encheiresis into superior veDa cava or subclavian vein．Results：One hundred and five cases were operated successflJlly at the first time．Non drug extravasation and intubation complication was observed in all cases．The degree of satisfaction is 1100％ ．Conclusion：PICC treated in early trauma patients can protect
the patients’blood vessels，reduce the pain induced by repeated puncture，and improve therapeutic and nursing quality．

Research into the Promotion of Practice Teaching Quailty in Local Universitie

2011, 10(6):  86-88. 

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Objective：This paper introduced the measures ofpractice teaching strategy ofpharmacy major in Dali University．Methods：It was essential to base on the l∞al area and to combine practice teaching closely with regional economy and social development．Courses should be oriented to the social and employment demands，as well as economic and social development．We reformed the practice teaching content of curriculum，improved the curriculum，textbook and teacher staffs．Results：Focused on training students’practical ability，we established the practical teaching platform to highlight the students’abilities in practice，creation and social adaption．Conclusion：With the above strategies，practice teaching courses have greatly improved students’practical，creative and social adaptation abilities．

Current Situation of Instructor Team Construction in Yuiman Medical Colleges

ZHANG Ai-Yun, LIU Qian-Qian, CHEN Ying

2011, 10(6):  92-94. 

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0bjectives：To investigate the current status and problems of the instructor team construction in yunnan medical coleges and to provide some specific recommendations Methods：Interviews and self-designed questionnaires were applied to college instrnetors from Kunming Medical University，Yunnan Traditional Chinese Medical College，Dali University and Yunnan Medical Higher Academy．Results：Despite some achievements，there are still such problems as irrational team structure，incomplete seleetion system．low management
performance，deficient training mechanisms，and non-objective assessment system．Conclusions：hnprovement of selection system，training system，management system and development system could solve the problems arising from the construction of college instructors team in Yunnan medical colleges．