J4 ›› 2011, Vol. 10 ›› Issue (6): 4-6.

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Determination of Gatifloxaein Level in Urine with Ultraviolet Spectrophotometry

Objective：To determine the content of Gatifloxacin in urine with ultraviolet spe(：trophotometry method．Methods：Urine samples from 5 healthy volunteers were collected．The contents of Gatifloxacin in unne were determined by ultraviolet speetrophotometry with the detective wavelength of 333 nm．Results：Data have shown a good linear relation between Gatifloxacin eotent and absorbance (r=0．999 8) with the Gatifloxacin concentration in 1．00 to 36．00 μg/mL．The average recovery was 98．1％with RSD 2．87％ ; the average
extraction recovery was 95．4％ with RSD 3．66％ ，excretion rate in 24 hours was 443％～69．6％ . Conclusion：The simple，quick，sensitive and accurate method applied here is worthy of promoting in Gatifloxacin determine in urine．   

  1. College ofPharmacy and Chemistry，Dali University，Dali，Yunnan 671000，China
  • Online:2011-06-01 Published:2011-06-01

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Abstract:

Objective：To determine the content of Gatifloxacin in urine with ultraviolet spe(：trophotometry method．Methods：Urine samples from 5 healthy volunteers were collected．The contents of Gatifloxacin in unne were determined by ultraviolet speetrophotometry with the detective wavelength of 333 nm．Results：Data have shown a good linear relation between Gatifloxacin eotent and absorbance (r=0．999 8) with the Gatifloxacin concentration in 1．00 to 36．00 μg/mL．The average recovery was 98．1％with RSD 2．87％ ; the average
extraction recovery was 95．4％ with RSD 3．66％ ，excretion rate in 24 hours was 443％～69．6％ . Conclusion：The simple，quick，sensitive and accurate method applied here is worthy of promoting in Gatifloxacin determine in urine．

Key words: gatifloxacin, ultraviolet spectmphotometfy, determinadon

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