Abstract:

Objective: To retrospectively analyze the changes of immunophenotyping of auto-cytokine induced killer cells from 59
patients with malignant solid tumor after amplification from January 2009 to May 2010, and to provide experimental evidence to CIK therapy for malignant solid tumor patients. Methods: Peripheral blood mononuclear cells（PBMC）of 59 patients with malignant solid tumor were collected. PBMC were cultivated for 10-14 days in vitro with traditional cultivating system added IFN-γ, CD3McAb and IL-2. Then, immunophenotype changes of CIK cells before and after culture were measured with flow cytometry （FCM）. The results then were conducted with matched t test. Results: After cultured, the PBMC, CD3 + cells, CD3 + CD4 + cells, CD3-CD56 + cells, CD3+ CD56+ cells increased significantly compared with that of before （P<0.05）. CD3+CD8+cells showed slight but not significant increase （P>0.05）. The percentage of CD3+, CD3+ CD4+,CD3-CD56+ phenotype cells declined with statistical significance （P<0.05）, while the percentage of CD3+CD8+ cells had no significant difference （P>0.05）. The purity of CIK cells after being cultured
（46.55±19.25）% was significantly higher （9.90±8.96）%. Conclusion: The purity of CIK cells from patients with malignant solid tumor increased significantly after amplification in vitro.

Key words: cytokineInduced killer cells, malignant solid tumor, immunophenotype