J4 ›› 2012, Vol. 11 ›› Issue (3): 28-30.

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Cloning and Sequencing of IpaB Gene of Shigella flexneri

Objective：To clone and sequence of ipaB gene of Shigella flexneri. Methods：IpaB gene was amplified by PCR. The DNA products of ipaB were inserted into a prokaryotic expression vector pQE-30, and then translated into E.coli strain Top10, restriction digestion and sequencing. Results：The recombinant plasmid was constructed and ipaB gene was sequenced as 1 743 bp, conformity with expectation. Conclusion：The results indicated that recombinant plasmid was constructed successfully.   

  1. Pre-clinical College, Dali University, Dali, Yunnan 671000, China
  • Received:2011-12-05 Online:2012-03-15 Published:2012-03-15

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Abstract:

Objective：To clone and sequence of ipaB gene of Shigella flexneri. Methods：IpaB gene was amplified by PCR. The DNA products of ipaB were inserted into a prokaryotic expression vector pQE-30, and then translated into E.coli strain Top10, restriction digestion and sequencing. Results：The recombinant plasmid was constructed and ipaB gene was sequenced as 1 743 bp, conformity with expectation. Conclusion：The results indicated that recombinant plasmid was constructed successfully.

Key words: Shigella flexneri, ipaB gene, cloning, assay, conformity

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