J4 ›› 2012, Vol. 11 ›› Issue (3): 31-34.

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Extraction and Determination of Polysaccharides from Yunlong Auricularia auricular

Objective: To extract both artificial culture and wild Auricularia auricular polysaccharides and determinate their
contents. Methods: The polysaccharide was extracted with ethanol and the polysaccharide content was wired by UV spectrophotometry phenol -sulfuric acid color method. Results: Within the range of 8.0 -48.0 μg/mL, the recovery ratio of polysaccharide was 99.03% with RDS of 0.66% (n=6). The recovery ratio of polysaccharide in artificial culture and wild Auricularia auricular were 18.48% and 18.29%, respectively. Artificial culture and wild Auricularia auricular in the polysaccharide content was 9.21% and 8.20%, respectively. Conclusion: Wild Auricularia auricular has higher content of polysaccharides than that of culture artificially. Growing environment affects the contents of Auricularia auricula's polysaccharides.   

  1. 1. Collegle of Public Health, Dali University, Dali, Yunnan 671000, China;
    2. Pre-clinical Collegle, Dali University, Dali, Yunnan 671000, China
  • Received:2011-06-15 Revised:2011-07-23 Online:2012-03-15 Published:2012-03-15

Abstract:

Objective: To extract both artificial culture and wild Auricularia auricular polysaccharides and determinate their
contents. Methods: The polysaccharide was extracted with ethanol and the polysaccharide content was wired by UV spectrophotometry phenol -sulfuric acid color method. Results: Within the range of 8.0 -48.0 μg/mL, the recovery ratio of polysaccharide was 99.03% with RDS of 0.66% (n=6). The recovery ratio of polysaccharide in artificial culture and wild Auricularia auricular were 18.48% and 18.29%, respectively. Artificial culture and wild Auricularia auricular in the polysaccharide content was 9.21% and 8.20%, respectively. Conclusion: Wild Auricularia auricular has higher content of polysaccharides than that of culture artificially. Growing environment affects the contents of Auricularia auricula's polysaccharides.

Key words: Auricularia auricula, ethanol extraction, UV spectrophotometry phenol-sulfuric acid method, polysaccharides, content

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