J4 ›› 2012, Vol. 11 ›› Issue (9): 98-100.

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Determination of Chlorogenic Acid Content in Xiaoerjinqiao Granules by RP-HPLC

Objective: To establish a HPLC method for content determination of chlorogenic acid in Xiaoerjinqiao Granules.
Methods: We applied the Agilent XBP-C18（4.6 mm×250 mm, 5μm）as the chromatographic column, and the mobile phase with acetonitrile-0.4% phosphoric acid solution（12:88）at the flow rate of 1.0 mL/min under the detection wavelength of 327 nm, and the column temperature of 25 0C. Results: There was a good linear relationship in the concentration range of 0.05~5.00 μg/mL （r =1.0）. The average recovery was 100.44% and RSD was 1.50%. Conclusion: The method appears to be simple, accurate and reliable, and can be used for the quality control of Xiaoerjinqiao Granules.   

  1. College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China
  • Received:2012-03-20 Revised:2012-05-22 Online:2012-09-15 Published:2012-09-15

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Abstract:

Objective: To establish a HPLC method for content determination of chlorogenic acid in Xiaoerjinqiao Granules.
Methods: We applied the Agilent XBP-C18（4.6 mm×250 mm, 5μm）as the chromatographic column, and the mobile phase with acetonitrile-0.4% phosphoric acid solution（12:88）at the flow rate of 1.0 mL/min under the detection wavelength of 327 nm, and the column temperature of 25 0C. Results: There was a good linear relationship in the concentration range of 0.05~5.00 μg/mL （r =1.0）. The average recovery was 100.44% and RSD was 1.50%. Conclusion: The method appears to be simple, accurate and reliable, and can be used for the quality control of Xiaoerjinqiao Granules.

Key words: Xiaoerjinqiao Granules, chlorogenic aci RP-HPLC

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