Table of Content

15 September 2012, Volume 11 Issue 9

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Chemical Constituents of Holotrichia diomphalia Bates

LI Lin, LI Xiao-Nian, WEI Shu-Si, YAN Yong-Meng, ZHONG Hui-Min, CHENG Yong-Xian

2012, 11(9):  1-4. 

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Objective: To investigate the chemical constituents of medicinal insects of Holotrichia diomphalia Bates. Methods:
Various chromatography materials and technology （including silica gel, RP-18 reverse phase silica gel, Sephadex LH-20, MCI gel CHP 20P, D101 macroporous adsorption resin column chromatography and high performance liquid chromatography）, combined with spectroscopic methods （NMR, MS, ORD）were applied to separate and identify the compourds of H. diomphalia Bates. Results: Ten compounds were isolated from H. diomphalia. Their structures were respectively identified as trans-1, 2-cyclohexanediol（1）,（9Z）-glycerin-1-monooleate（2）, salicin（3）, 4-methoxyphenyllacetic acid（4）, glycerin-1-benzoate（5）, yangjinhualine A（6）,（±）-dimerthyl-2（（phenylmethoxy）methoxy）pentanedioate（7）, salicyl alcohol（8）,（p-hydroxyphenyl）acetic acid（9）, and oxiran-2-ylmethanol （10）by a suit of spectroscopic techniques. Conclusion: All the compounds were isolated from H. diomphalia for the first time.

In Vitro Antibacterial Activity of Extracts from Blaps rynchopetera Fairmaire

SHI Gui-Rong, ZHUANG Xiao-Long, GENG Ling, WU Xiu-Mei, LUO Jian-Rong, LIU Guang-Ming

2012, 11(9):  5-7. 

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Objective: To explore the in vitro antibacterial activity of different polar parts of Blaps rynchopetera Fairmaire.
Methods: The extracts were obtained by column chromatography on silica gel. Then, we measured the MIC and MBC of different components in extracts to six clinical pathogenic bacteria by tube dilution method. Results: Ethyl acetate showed stronger antibacterial activity in vitro than acetone extract. There were no antibacterial activity of ligroin, chloroform and methanol extract. Conclusion: B. rynchopetera Fairmaire exhibited good antibacterial effect in vitro, ethyl acetate extract is the key antibacterial activity site.

Optimization of the Extraction Process of Bergenin from Rodgersia pinnata

WANG Yan, JIN Yang, LIU Zhen-Qi

2012, 11(9):  8-10. 

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Objective: To optimize the extraction technology of bergenin from Rodgersia pinnata Hemsl. Methods: The extraction
technology of bergenin from Rodgersia pinnata Hemsl was optimized by orthogonal design taking the content of bergenin as index with the solvent concentration, the amount of solvent, the extraction time and extraction times as investigation factors. Results: The optimal extraction technology of bergenin from Rodgersia pinnata Hemsl was as 60% methanol as solvent, 20 mL of solvent amount, 20 min of extraction time, and 3 times of extraction. Conclusin: The optimum extracting technology is simple, feasible, stable and reproducible,and it serves as theoretical reference for further development and utilization of Rodgersia pinnata Hemsl.

Domestic Gel Paste Patent Analysis

ZHANG Cheng-Gui, HE Miao, WU Xiu-Mei, ZHAO Yu, GAO Peng-Fei

2012, 11(9):  11-15. 

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More than eighty published patent applications and fifty authorized patents related to gel paste were retrieved from the
patent databases of State Intellectual Property Office of the People's Republic of China. They were categorized into 18 classes of gynaecology, nourishing kidney and reducing tiredness, mammary diseases, hepatocirrhosis ascites, detection methods, hypoglycemic agents, thyroid diseases, cardiovascular diseases, respiratory diseases, gastrointestinal diseases, anorectal diseases, Alzheimer's disease, depression, antimicrobial peptides, analgesia and traumatic injury, manufacture methods, reforms of formulation, and patents of utility models with respect to their purposes. With special emphases on the authorized patents, these patent applications were summarized and analyzed with the aim of furnishing more plentiful information and critical suggestions on relative ongoing R&D projects concerned on gel paste.

Patent Study of Miao Nationality Drug Cynanchum wilfordii (Maxim.) Hemsl and Bai/Yi

TANG Jun, LI Ting, WU Xiu-Mei, GENG Ling, LI Feng-Xian, ZHAO Yu, LI Hui

2012, 11(9):  16-21. 

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This paper analytically recorded Chinese patents during last two decades on the medicinal applications of Cynanchum
wilfordii (Maxim.) Hemsl as a Miao nationality herbal drug and Geranium strictipes R. Knuth as that of Bai/Yi nationalities. The two herbs from different families possess the same popular name of Geshanxiao. Their origins, varieties, nomenclature, folk conventional usage, major functions, and their compatibility principles are compared and summarized in order to furnish more information and suggestions on relative on-going R&D projects of these two herbal drugs.

Remineralization of Artificial Demineralized Enamel by Extracts from Galla Chinensis and Galla Halepensis

CHEN Kun, RUAN Jin-Yun, YIDuo-Zong, YANG Fa-Cheng, LAN Hai

2012, 11(9):  22-24. 

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Objective: To detect the hardness of artificial demineralized enamel treated with ethanol extract of Galla Chinensis and
Galla Halepensis. Methods: Caries models were established using chemical methods and treated with the extracts of Galla Chinensis and Galla Halepensis. The surface hardness was determinated using micro hardness tester. Results: The average surface hardness values are 345.4 kg/mm², 330.6 kg/mm² and 313.0 kg/mm² respectively under the treatments of extracts of Galla Chinensis, Galla Halepensis and deionized water. Conclusion: Data showed both remineralizing capacities of Galla Chinensis extract (80 mg/mL) and Galla Halepensis extract (80 mg/mL). The Galla Chinensis extract exhibited stronger effects compared to that of Galla Halepensis extract.

In vitro Study of the Effect of Scallop Shells Extract on Fat Degradation

YAN Xu-Yi, LIU Yun-Chun

2012, 11(9):  25-28. 

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Objective: To investigate the effect of scallop shells on fat degradation and find new ways to increase their added value.
Methods: To evaluate the effect of bioactive substances of scallop shells on fat degradation, 3T3-L1 cells were treated with methanol extract of scallop shells, the amounts of glycerol secreted from 3T3-L1 cells were measured and the fat contents in 3T3-L1 cells were observed by Oil Red O staining. Results: 3T3-L1 cells treated with scallop shell extract secreted more glycerol (P<0.05) and were with less fat. Conclusion: This study provided evidence that bioactive substances in scallop shells promote fat degradation, which suggests a potential value of scallop shells.

Protective Effect of Xinmailong Capsules on Morphologic Injury in Primary Cultured Rat Vascular Smooth Muscle Cells

XIAO Fen, WU Jian-Xin

2012, 11(9):  29-31. 

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Objective: To investigate the primary culture method of rat aorta VSMCs and observe the protection of XMLJ on
morphologic injury of VSMCs indcued by H₂O₂. Methods: Rat aorta VSMCs were cultured following tissue-piece inoculation method. Cultured VSMCs were then stimulated with 62.5 μmol·L^-1 H₂O₂ under the condition of with or without XMLJ, HE dyeing method was applied to observe the morphologic changes of VSMCs. Results: With XMLJ 200 μg·mL^-1, VSMCs under oxidative stress exhibited nearly no difference compared with the normal cells. XMLJ with 200 μg·mL^-1 on VSMCs morphologic change induced by oxidative stress showed obvious protective effect.Conclusion: Tissue-piece inoculation culture of VSMCs is uncomplicated. There was a protective effect of XMLJ on morphologic injury of VSMCs.

Effect of Semen Litchi fulvosus YQ Lee on the Gastrointestinal Function

GAO Chun, DU Zhong-Fu, YANG Shao-Long, HE Lin, ZHANG Cui, FANG Chun-Sheng

2012, 11(9):  32-34. 

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Objective: To study the effect of Semen Litchi fulvosus YQ Lee on the gastrointestinal function of animals. Methods:
The effect of Semen Litchi fulvosus YQ Lee on gastric emptying and intestinal propulsion of mice, as well as the excitability of isolated ileum muscle of guinea pig were determined. Results: Semen Litchi fulvosus YQ Lee could significantly inhibit the gastric emptying and small intestinal propulsion on mice with dose-dependent manner. In addition, Semen Litchi fulvosus YQ Lee could obviously inhibit the excitability of ileum induced by acetylcholine, histamine and BaCl2 in vitro. Conclusion: Semen Litchi fulvosus YQ Lee could restrain the gastrointestinal motor function on mice and guinea pig.

Study on Quality Control of Several Processed Products of Strychnos

WANG Jing, DENG Fu-Wen, JIANG Yan-Ting, ZHANG Yi-Zhu, DUAN Cha-Feng

2012, 11(9):  35-37. 

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Objective: Strychnos content of active ingredients before and after processing and the optimal processing method.
Methods: To strychnine as a reference substance, was measured at 262.6 nm wavelength of Strychnos alkali content in. Results: The brucine showed good linearity with in the range of 2.2~13.2 mg/mL, r=0.999 5, the average recovery was 92.88% (RSD=4.3%).Conclusion: The method is simple, accurate, and can be used for Strychnos quality control of processed products.

Preliminary Study of In Vitro Antimicrobial Effects of Extracts from Juglans sigillata Shell

ZHANG Hai-Zhu, MA Yuan, ZHOU Ping

2012, 11(9):  38-40. 

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Objective: To study the antibacteria effect of Juglans sigillata Shell extract in vitro. Methods: Micro dilution method was
applied to analyze the antibacterial effect of different extracts from Juglans sigillata shell to Pseudomonas aeruginosa, Bacillus dysenteriae, Staphylococcus aureus, Bacillus subtilis, Proteus mirabilis, Escherichia coli, Salmonella typhi, Candida albicans in vitro. The indexes of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were measured. Results: Extracts exhibited sterilization or antibacterial effect to 8 strains of bacteria. Conclusion: All different solvent abstracts of Juglans sigillata sigillata shell showed obvious antibaeteria effects.

In vitro Study on Antifungal Susceptibility of Vaginal Candida from Healthy Population in Dali

HE Wei, CHEN Cai-Fa, YAO Yi, ZHU Wen, YANG Ze-Li, BAI Li

2012, 11(9):  41-43. 

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Objective: To provide a basis for clinical selection of antifungal drugs through vaginal candida antifungal susceptibility
test. Methods: We measured the vaginal candida antifungal susceptibility (minimal inhibitory concentration, MIC) to fluconazole (FCZ), ketoconazole (KCZ), 5-fluorocytosine (5-FC) and amphotericin B (AMB) by using Broth microdilution method. Results: The resistance rates of 45 strains vaginal candida from healthy subjects to antifungal drug FCZ, KCZ, 5-FC, AMB were 8.89%, 6.67%,6.67% and 2.22% respectively. And the sensitive rates were 80%,84.44%, 91.11%, 97.78% respectively. One strain of C. krusei is resistant to FCZ, KCZ and 5-FC while another strain was simultaneous resistant to KCZ and 5-FC. There were no significance differences in all 45 strains of candida in antifungal drugs resistant rate. The sensitivity of Candida to AMB was higher than that of FCZ (P=0.008). The sensitivity of Candida to AMB was higher than that of KCZ (P=0.027). There were no significant differences among rest drugs in sensitive rate (P>0.05). Conclusion: The above four kinds of commonly used clinical antifungal drugs can be selected to treat vaginal candidasis, but should be aware that some strains are sensitive and resistant with dose-dependent manner.

Protective Effect and Mechanism of Weinaian Capsule on Gastric Mucosal Lesion Induced by Alcohol in Mice

ZHANG Lei, GUO Shuang-Yan, TAO Li-Jun, MA Li-Juan, BAI Xiao-Ping, QIAN Xiao-Hua, XU Guo-Ping

2012, 11(9):  44-46. 

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Objective: To investigate the protective effect of Weinaian capsule on gastric mucosal lesion in mice induced by alcohol.
Methods: Mice were randomly divided into normal group, model group, low dose group and high dose group. The lesion index of mice were measured and pathological changes of gastric mucosa were assessed under light microscopy 1 hour after alcohol inducement. Results: Gastric mucosal lesion indexes in high dose group and low dose group were both obviously reduced compared to that in the model control group, and the gastric mucosal lesions in those two experimental groups significantly alleviated compared to that of the control group under light microscopy. Conclusion: Weinaian capsule could significantly protect acute gastric mucosal lesion induced by alcohol in mice.

Application Progress of Immunolabelling Technique in the Diagnosis of Angiostrongyliasis Cantonensis

YANG Yi-Mei, ZHANG Xiao-Xiao, CUI Li-Yun, WANG Fei, CAO Shu-Zhen

2012, 11(9):  47-50. 

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Objective: To review the application progress of immunolabelling technique in the diagnosis of angiostrongyliasis
cantonensis. Methods: The related literatures about immunolabelling technique in the diagnosis of angiostrongyliasis were analyzed and summarized. Results: Different immunolabelling techniques in the diagnosis of angiostrongyliasis showed diverse efficacies, we should choose different diagnostic methods under different circumstances. Conculsion: Immunolabelling technique was with great significant potential in the diagnosis of angiostrongylisis cantonensis, however more improvement of new immunolabelling techniques is needed.

Practical Assessment of ALT Primary Screening Test Before Blood Collection of Unpaid Blood Donors in Dali Area

LIU Zi-Hua

2012, 11(9):  51-52. 

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Objective: To evaluate the practical assessment of alanine amino transfer enzyme （ALT）primary screening test before the blood collection of blood donors in the Dali area. Methods: Unpaid blood donors from 2010 to 2011 in our station were enrolled, ALT results were analyzed. Results: After the ALT primary screening test was applied, ALT positive scrap rate was deceased, the difference was statistical significant. Conclusion: ALT screening test before blood collection could reduce effectively blood scrap rate.

Comparative Study of the Efficacy of Three Surgical Treatments of Osmidrosis

XU Xiao-Guang, WANG Yang, LIU Xiao-Bo, ZHAO Qing-Lin

2012, 11(9):  53-55. 

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Objective: To compare the efficacy of traditionary fusiform-shape excision, micro incision curettage and radiofrequency
ablation in the treatment of osmidrosis. Methods: One hundred and eighty-six patients with osmidrosis were treated by traditionary fusiform shape excision method （20 cases）, micro incision curettage （116 cases）, and radiofrequency ablation （50 cases）. The therapeutic effects and complications were retrospectively analyzed. Results: The patients were followed up for 3 months to 1 year. The cure rate was 100%, 97.7% and 68.4%, respectively. Conclusion: All three methods are effective for the treatment of osmidrosis. The odor symptom could be improved a lot with traditionary fusiform shape excision method, which however would cost lots on complication, aesthetic improvement and convalescent period. The radiofrequency ablation was with smallest injuries and high relapse rate. Micro incision curettage was efficacy and aesthetic.

Curative Effect Analysis of Elderly Unstable Intertrochanteric Fracture with PFNA

MA Li-Wu, ZHANG Ji

2012, 11(9):  56-58. 

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Objective: To evaluate proximal femoral nail anti-rotation（PFNA）in the treatment of unstable intertrochanteric fracture.
Methods: The classifications, operation approachs and post-operative effects were assessed in the 22 patients of intertrochanter fracture of femur treated with PFNA. Results: All cases were followed up from 11 to 26 months after operations（average 13 months）.The post-operative follow-up study showed that all the cases were fracture healed, under the joint scores, 16 cases were excellent （73%）, 4 cases were good （18%）, 2 cases were normal （9%）, the excellent rate was 90.9%. Conclusion: Treatment of elderly unstable intertrochanteric fracture of with PFNA was an ideal approach with simple operation, less trauma, reliable fixation.

Clinical Application of Acute Renal Failure Followed Upper Ureteric Obstruction with Ureteroscopy

DONG Xue-Yi, ZHAO Heng-Tai, LUO Zhong-Jie, ZHU Wen-Hao, WANG Yong-Xiong, ZHONG Li-An

2012, 11(9):  59-61. 

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Objective: To investigate the treatment of acute renal faiure (ARF) due to upper uretericobstruction. Methods: Fifty-six
patients with ARF followed upperureteric obstruction were treated with ureteroscopy, combined with pneumatic lithotripter in case of urinary tract stone, and placed double J tube for drainage treatment. Results: The level of BuN and SCr decreased obviously after operation, and urine volume recoverd in different degrees. Conclusion: Ureteroscopy provides a safe and effective method for the treatment of acute renal failure followed upper ureteric obstruction, which could treat the bilateral ureteral obstruction.

300 Cases of IUD-therapy after Scar Uterus Abortion

LI Lian-Xiu

2012, 11(9):  62-63. 

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Objective: To explore the birthcontrol measurements after scar uterus abortion operation. Methods: Data from 300 cases of scar uterus abortion from January 2004 to December 2010 in our station were conducted a retrospective analysis. Results: Intrauterine device (IUD) therapy after artificial abortion was safe, good contraceptive effect, with less side-effect and high continuous using rate. Conclusion: IUD therapy is a safe and effective contraceptive measure, which can effectively promote women's reproductive health.

Clinical Application of Micro-implant Anchorage in Orthodontic

LIU Hong

2012, 11(9):  64-66. 

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Objective: To investigate the clinical application and effect of micro-implant anchorage in orthodontics. Methods:
Eleven cases with indications of maximum anchorage requirements were selected and conducted the micro -implant anchorage appliance therapy from June 2008 to February 2011, all documents were retrospectively analyzed. Results: All patients reached correction target after orthodontic treatment, and the results were satisfactory. Conclusion: Micro-implant anchorage, as a solid intraoral anchorage, could fully address the exist problems in clinical anchorage and achieve treatment goals and effects that is difficult for traditional method.

Management of General Anesthesia in Interventional Embolization Therapy in Cerebrovascular Disease

LIU Wen-Pei

2012, 11(9):  67-69. 

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Objective: To summarize the general anesthesia management in interventional embolization therapy in cerebrovascular
disease. Methods: Sixteen cases of cerebrovascular disease accepted general anesthesia were analyzed retrospectively, and the related literature was reviewed. Results: Sixteen cases were stable in haemodynamics during anesthetic process. Three cases of Hunt-Hess class 3-4 were nursed in ICU with endotracheal tube treatment after surgery. Eleven cases with spontaneous respiration and opened eyes in ten minutes after surgery were extubated successfully. Two cases treated with laryngeal mask airway (LMA) were removed immediately after surgery. All 13 patients who had been removed intubation/LMA were orientation recovery in fifteen minutes after surgery. Conclusion: Perfect evaluation and preparation before anesthesia, short-acting anesthetics, LMA application in Hunt-Hess class1-2 patients could ensure haemodynamics steady during surgery, patients could be recovery rapidly and smoothly after surgery,and capable to conduct early neurological assessment.

Endoscopic Treatment of Upper Gastrointestinal Calculus

DU Gui-Ying, TAO Zhong-Yuan, YANG Xiao-Ying, LU Ruo-Li

2012, 11(9):  70-72. 

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Objective: To explore the operating method and clinical effect of endoscopic therapy in the digestive tract calculus.
Methods: Forty-two cases of upper digestive tract calculus were conducted endoscopic interventional treatment and the stones were removed by repeated application of foreign body forceps, snare, basket and gastrolith cutter. Results: Thirty-four cases succeeded in once endoscopic lithotripsy, 6 cases succeeded with 2 times and 1 case with three times. The success rate was 97.62%. Complications occurred in 2 cases, accounting for 4.76%. Conclusion: Endoscopic therapy was a safe, simple and effective method in treatment of upper gastrointestinal calculus.

Pre-operative Visit and Post-operative Return Visit in Nursing

FENG Yu

2012, 11(9):  73-75. 

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Objective: To explore duty nurse's experience of pre-operative visit and post-operative return visit. Methods: The preoperative visit and post-operative return visit of surgical patients from January 2006 to December 2011 in our hospital were analyzed and summarized. Results: Pre-operative visit can strengthen the communication between nurses and patients and alleviate patient's mental stress; post-operative return visit could improve the understanding of the surgical effect and improve patient's recovery confidence. Conclusion: Pre-operative visit and post-operative return visit could increase the communication between nurse and patient and help to formulate better care measurements. It would be helpful to patients.

Taking Effective Measures and Improving Comprehensively Higher Education Quality

DAI Zhi-Ming

2012, 11(9):  76-82. 

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Overall improvement of the higher education quality is a major decision made by the CPC Central Committee and the
State Council. To carry out General Secretary HU Jintao's important speech on Qinghua University centennial anniversary, as well as "National long-term educational reform and development plan （2010-2020）", the Ministry of Education issued "Several opinions on overall improvement of higher education quality" and proposed 30 specific measures. This document is the guidance to comprehensively improve the quality of higher education in the new historical period. Our university should attach great importance to take effective measures to implement under the requests on the documents.

Reform Pharmacognosy Teaching Model and Culture High Quality Pharmaceutical Student

ZHOU Nong, XIA Cong-Long, YANG Yue-E , ZHANG De-Quan, DUAN Bao-Zhong, JIANG Bei

2012, 11(9):  83-86. 

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Objective: To improve the effects of pharmacognosy teaching and culture pharmaceutical student with solid basic
knowledge of pharmacognosy. Methods: In view of the training goal and curriculum requirements for students at different levels, we appropriately revised the teaching plan and contents of pharmacognosy and improved the teaching skills and methods. Results: Student's interests were stimulated and teaching effects were enhanced. Conclusion: It is helpful to improve the teaching effects, and the reformation greatly promoted the professional development of students.

On the Necessary Skills of the Instructor of Medical Student

ZHANG Hui, LI Ding-Kun

2012, 11(9):  87-90. 

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Medical student is a special group with distinct characteristics on campus. This calls for different requirements to medical student's instructor, who share some general features with ordinary college instructor but diverse with different characteristics. Medical student's characteristics were analyzed, the document "Several opinions on further enhancing and improving college student's ideological education" issued by the State Council was learned, and the necessary skills a medical student instructor needs were analyzed. New working methods were expected to be found to improve the working skills and levels of the medical student instructor.

Characteristics of the Biology Exam Question of Yunnan Provincial Science Comprehensive Exam for 2012 College

QIN Jun-Yi

2012, 11(9):  91-94. 

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This paper intends to explore the characteristics of the biology exam question of 2012 College Entrance Examination
under the new curriculum standard and leads to a critical thinking on the teaching of biology.

Determination of Chlorogenic Acid in Sangjiangganmao Capsules by RP-HPLC

LI Sheng-Nan, HAN Ben-Xu, LIU Sheng-Xu, XIAO Pei-Yun

2012, 11(9):  95-97. 

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Objective: To establish a RP-HPLC method for the determination of chlorogenic acid in Sangjiangganmao capsules.
Methods: An Agela XBP-C18 (250 mm×4.6 mm, 5 μm) column was applied, the mobile phase was acetonitrile-0.4% phosphoric （9:91）, the detection wavelength was 327 nm with the flow rate of 0.1mL/min. Results: There was a good linear relationship in the chlorogenic acid concentration range of 0.02-0.25 μg (r=0.999 8). The average recovery was 99.5%, RSD=0.67% (n=6). Conclusion: The method was with good repeatability, flexibility and high sensitivity, which can be used in the quality control of Sangjiangganmao capsules.

Determination of Chlorogenic Acid Content in Xiaoerjinqiao Granules by RP-HPLC

YANG Li-Bo, HUANG Li, DU Hong, YANG Yong-Shou

2012, 11(9):  98-100. 

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Objective: To establish a HPLC method for content determination of chlorogenic acid in Xiaoerjinqiao Granules.
Methods: We applied the Agilent XBP-C18（4.6 mm×250 mm, 5μm）as the chromatographic column, and the mobile phase with acetonitrile-0.4% phosphoric acid solution（12:88）at the flow rate of 1.0 mL/min under the detection wavelength of 327 nm, and the column temperature of 25 ⁰C. Results: There was a good linear relationship in the concentration range of 0.05~5.00 μg/mL （r =1.0）. The average recovery was 100.44% and RSD was 1.50%. Conclusion: The method appears to be simple, accurate and reliable, and can be used for the quality control of Xiaoerjinqiao Granules.