J4 ›› 2013, Vol. 12 ›› Issue (6): 12-16.

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Extraction, Purification, Structure Characteristics and Anticoagulation of the Sulfated Polysaccharides from
the Sea Cucumber Holothuria nobilis

  

  1. 1. College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China; 2. State Key Laboratory of Phytochemistry and
    Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
  • Received:2013-03-18 Revised:2013-04-06 Online:2013-06-15 Published:2013-06-15

Abstract:

Objective: To study the polysaccharide types, physicochemical properties and anticoagulant activities of sulfated
polysaccharides from the sea cucumber Holothuria nobilis Selenka. Methods: Sulfated polysaccharides were purified by methods of
chemistry and physics. Physicochemical properties and biological activities of the saccharides, such as purity, chemical composition,
molecular weight, intrinsic viscosity, optical rotation and anticoagulant activities were determined by methods of chromatography,
chemistry, spectrum technologies, etc. Results: The polysaccharides were separated into two fractions, F-I and F-II, respectively. The
components of F-I were identified as glucuronic acid, aminogalactose, fucan and sulfate (molar ratio 1.00:0.96:1.18:2.99); F-II
contained only fucan and sulfate (molar ratio 1.00:0.51). The two polysaccharides were significantly different in the physicochemical
properties, and F-I had 6 times of anticoagulant potency compared to the F-II. Conclusion: The sea cucumber H. nobilis contains at
least two types of sulfated polysaccharides, a fucosylated glycosaminoglycan sulfate and a sulfated α-L -fucan which have
anticoagulant activities.

Key words: sea cucumber, polysaccharides, purification, physico chemical characteristics, anticoagulant activities

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