Determination of Chlorogenic Acid in Alnus ferdinandi-coburgii by HPLC

Abstract

Abstract:

Objective: To establish an HPLC method for the determination of chlorogenic acid in Alnus ferdinandi -coburgii.
Methods: We applied highly effective liquid phase chromatography, with Venusil X BP-C18 column （150 mm×4.6 mm,5 μm）and
mobile phase consisted of acetonitrile-0.4% phosphoric acid solution（8:92）at a flow rate of 1.0 mL/min. The detection wavelength was
set at 324 nm, and column temperature was 25 ⁰C. Results: There was good linearity in the range of 0.108 8-1.088 μg （r=0.999 9）
of chlorogenic acid. The average recovery was 98.98%, RSD was 0.77%（n=6）. Conclusion: The method above was simple, sensitive,
accurate, reproducible, and could be applied in the measurement of chlorogenic acid in Alnus ferdinandi-coburgii.

Key words: Alnus ferdinandi-coburgii, chlorogenic acid, HPLC

CLC Number:

R284

YIN La-Mei, YANG Xiao-Zhen, CAO Yan-Hua, LIU Ting, XIAO Pei-Yun. Determination of Chlorogenic Acid in Alnus ferdinandi-coburgii by HPLC[J]. J4, 2013, 12(6): 20-22.

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Determination of Chlorogenic Acid in Alnus ferdinandi-coburgii by HPLC

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