Abstract:

Objective: To determine the content of the momordicoside in Morrnordica charantia L. wine. Methods: Vanillin -
perchloric acid colorimetry was applied to determine the contents of the total saponin in Morrnordica charantia L. wine, ginsenoside
and β-sitosterol were used as the reference substance. Results: The concentration of ginsenoside had good linearity in the range of
0.030-0.240 mg（r=0.999 1）. The average recovery rate was 99.6%, RSD=2.5%（n=6）, the content of sample was 0.151 1-0.563 0 mg.
The concentration of 茁-sitosterol had good linearity in the range of 0.015-0.116 mg（r=0.999 3）. The average recovery was 100.9%，
RSD= 2.6% （n=6）, the content of sample was 0.065 6-0.247 9 mg. Conclusion: β-sitosterol and ginsenoside are suitable for
reference substance to determine the content of saponin in Morrnordica charantia L. wine. The method was accurate which could be
used for the quality control of Morrnordica charantia L.Wine.

Key words: UV, momordicoside, contents determination