J4 ›› 2014, Vol. 13 ›› Issue (10): 5-9.

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Experimental Study on Anti-HSV-2 Effect of CII-3 Extracted from Periplaneta Americana
in Vitro and in Vivo

  

  1. 1. College of Pharmacy and Chemistry, Dali University, Dali, Yunnan 671000, China; 2.Yunnan Provincial Key Laboratory of
    Entomological Biopharmaceutical R&D, Dali, Yunnan 671000, China; 3.College of Foreign Languages, Dali University, Dali, Yunnan
    671003, China; 4.People's Hospital of Mangshi, Dehong, Yunnan 678400, China
  • Received:2014-01-08 Online:2014-10-15 Published:2014-10-15

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Abstract:

Objective: To study the anti-HSV-2 effect of CII-3 extracted from Periplaneta Americana. Methods: Anti-HSV-2
activity of CII-3 extracted from Periplaneta Americana was observed and determined with MTT assay and cytopathic effect (CPE) assay
and the value of TC50, IC50 and TI in the extract was calculated. Female mice with genital herpes were used as animal models. 50 μL of
HSV- 2 was injected into mice vaginas once a day with 7 consecutive days. With acyclovir as the positive control, intragastric
administration was adopted as treatment for 10 days. The ELISA KIT was used to test the concentration of vascular tumor necrosis
factor-α (TNF-α), Interferon-γ (IFN-γ) and Interleukin-2 (IL-2); the immune organs were weighed to calculate the organ index.
Results: CII-3 extracted from Periplaneta Americana had anti-HSV-2 activity. The TC50 value was 190.55 μg/mL, IC50 value was
28.71 μg/mL and TI value was 6.64 μg/mL. CII-3 had the function of increasing TNF-α, IFN-γ, IL-2 and could increase the weight
of the immune organs in mice. Conclusion: CII-3 extracted from Periplaneta Americana had the effect of anti-HSV-2 by boosting the
body's immunity.

Key words: Periplaneta Americana extract, HSV-2, antiviral effect

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