Abstract:

Objective: To study the analysis method of capillary gas chromatography in determination of sodium cyclamate content in milk and to lower the detection limit of the method by optimizing the settling agent dosage, esterification time and other conditions.Methods: Sodium cyclamate reacted in the medium of sulfuric acid with sodium nitrite and cyclohexanol nitrite was produced and extracted with n-hexane, and its content was determined by capillary gas chromatography. Results: Linear range was from 0.05 to 0.4 mg/mL, the detection limit was 2 μg, recovery of standard addition was 99.4%-102.5%, and RSD was 1.4%. Conclusion: The method was simple, rapid and accurate, with good reproducibility and low detection limit, much better than detection limit of 4 μg by GB/T5009.97-2003 method.

Key words: sodium cyclamate, milk, gas chromatography, capillary column