Abstract:

Objective: To research the effect of Periplaneta americana de-fat extract on immune function in normal mice and in
immunocompromised mice caused by cyclophosphamide. Methods: In this study, immunosuppressive model was established in C57BL/6
mice by cyclophosphamide. After the high, medium and low dose（4 g/kg, 2 g/kg, 1 g/kg）of P. americana de-fat extract were orally
administered for 15 d, T and B lymphocyte proliferation was measured. And the organ weights and organ index, the number and
classification of peripheral blood cell, bone marrow nucleated cell were tested. Results：P. americana extract can promote the
peoliferrative function of splenic linphocytes, increase the weight of immunocompromised mice organs and organ index, improve their
peripheral blood, and increase the number of bone marrow cells to enhance their immune function. Conclusion: The P. americana defat
extract can improve and influence the immune function on immunocompromised mice, but it has no effect on the immune function of
normal mice.

Key words: Periplaneta americana extract, immunoloregulation, lymphocyte proliferation, organ index, peripheral blood cell patten