Abstract:

Objective: To analyze the content of puerarin, daidzin and genistein from roots, stems and leaves of Pueraria mirifica, and
to explore the best effective parts. Methods: HPLC with the Hypersil BDS C18 column（250 mm×4.6 mm, 5 μm）was adopted.
Mthanol and water were used as mobile phase of gradient elution, with the flow rate of 0.7 mL/min. The injection volume of the sample
was 10 μL. The column temperature was 25 ℃, and the detection wavelength was 250 nm. Results: The descending order of puerarin
content in the samples of different parts of P. mirifica was root（0.531 ± 0.089）% > stem（0.024 ± 0.006）% > leaf（0.018 ± 0.004）%.
The descending order of daidzin content was root（0.226 ± 0.034）% > leaf（0.135 ± 0.009）% > stem（0.041 ± 0.006）%. The
descending order of genistein content was root（0.075 ± 0.001）% > stem（0.054 ± 0.007）% > leaf（0.016 ± 0.003）%. Conclusion:
Values of the three secondary metabolites in roots of P. mirifica is the highest. Content of puerarin and genistein in stems are higher
than them in leaves. The content of daidzin in leaves is higher than it in stems.

Key words: Pueraria mirifica, HPLC, different parts, secondary metabolites