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Table of Content

    15 August 2015, Volume 14 Issue 8
    The Influence of Different Polar Parts of Pinus yunnanensis on Trachea in Vitro of Guinea Pigs in Different State
    Guo Meixian, Liu Guangming, Shen Lei, Li Dongmei, Zhao Jianrong, Yang Ji
    2015, 14(8):  1-4.  DOI: 1672-2345.2015.08.001
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    Objective: To observe the spasmolytic action of different polar parts of Pinus yunnanensis on trachea in vitro of guinea
    pigs in different state. Methods: Using the methods of trachea in vitro, the influence of different polar parts of P. yunnanensis on
    trachea in vitro in resting state and acetylcholine(Ach)or histamine(His)induced spasm state were observed. Results: Ethanol
    extract, petroleum ether and chloroform parts of P. yunnanensis can reduce the tension of trachea in vitro in resting state and
    significantly increase basic spasmolysis rate; Ethanol extract, petroleum ether, chloroform and ethyl acetate parts of P. yunnanensis can
    reduce the tension of trachea in vitro in the Ach, His induced spasm state and significantly increase spasmolysis rate of spasmodic
    trachea. Conclusion: Ethanol extract, petroleum ether and chloroform part of P. yunnanensis can improve the spasmolysis rate of
    trachea in vitro of guinea pigs in different state, and relax resting and spasmodic trachea.

    Determination of Moistures, Total Ashes, and Ethanol Extracts from Incarvillea mairei
    Tang Yifang, Chen Pengfei, Duan Hongfan, Wu Jinjie, Wang Weiwei, Jiang Wei, Yang
    2015, 14(8):  5-7. 
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    Objective: To determine the moisture, ashes, and extracts from the Incarvillea mairei to establish the quality standards.
    Methods: According to the procedures recorded in the Appendix IX H, IX K and X A of 2010 Chinese Pharmacopoeia, moisture,
    ashes, and extracts of samples were measured respectively. Results: Combining the results of determination, the moisture of Incarvillea
    mairei was less than 8.0%; the total ashes was less than 6.5%, with the acid insoluble ash content was less than 2.0%, with hot sock
    method, the content of ethanol extract was higher than 7.5%. Conclusion: The methods can provide reference basis for the Incarvillea
    mairei quality standards.

    Study of Secondary Metabolites from Different Parts of Pueraria mirifica
    Ma Shuyun, Chu Jiming, Li Haifeng, Xu Li
    2015, 14(8):  8-10. 
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    Objective: To analyze the content of puerarin, daidzin and genistein from roots, stems and leaves of Pueraria mirifica, and
    to explore the best effective parts. Methods: HPLC with the Hypersil BDS C18 column(250 mm×4.6 mm, 5 μm)was adopted.
    Mthanol and water were used as mobile phase of gradient elution, with the flow rate of 0.7 mL/min. The injection volume of the sample
    was 10 μL. The column temperature was 25 ℃, and the detection wavelength was 250 nm. Results: The descending order of puerarin
    content in the samples of different parts of P. mirifica was root(0.531 ± 0.089)% > stem(0.024 ± 0.006)% > leaf(0.018 ± 0.004)%.
    The descending order of daidzin content was root(0.226 ± 0.034)% > leaf(0.135 ± 0.009)% > stem(0.041 ± 0.006)%. The
    descending order of genistein content was root(0.075 ± 0.001)% > stem(0.054 ± 0.007)% > leaf(0.016 ± 0.003)%. Conclusion:
    Values of the three secondary metabolites in roots of P. mirifica is the highest. Content of puerarin and genistein in stems are higher
    than them in leaves. The content of daidzin in leaves is higher than it in stems.

    Optimization of Matrix Composition for Astragalus Polysaccharides Skincare Product
    Zhang Yao, Li Wenhu, Sun Yingying, Yan Ya
    2015, 14(8):  11-14. 
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    Objective: To optimize the matrix composition of Astragalus polysaccharides skincare products. Methods: The stability
    and appearance properties of Astragalus polysaccharides skincare product were used as the targets of evaluation, the HLB value of
    emulsified oil phase, the emulsifying temperature, and the preparation methods were optimized by using single- factor test. The
    influences of the ratio of oil phase and water phase and the emulsifiers HLB value on emulsifiable paste were explored by using L9(34)
    orthogonal experiment design. Results: The optimum matrix composition was the oil phase of stearie acide : liquid paraffin : white
    vaseline = 1.0 : 1.7 : 0.9,the emulsifiers HLB value 13, the emulsify temperature 80 °C, and the water phase of glycerol : triethylolamine :
    water = 7.0 : 15.0 : 1.0. Conclusion: The formulation of Astragalus polysaccharides skincare product is suitable, the quality is stable,
    and the preparation method is simple.

    In Vitro Anti-tumor Effects of Extracts from Juglans sigillata Shell
    Liu Chengjiao, Xia Mengkai, Li Yang, Wang Ying
    2015, 14(8):  15-17. 
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    Objective: To study the anti-tumor effect of Juglans sigillata shell extracts on three human tumor cell lines including
    SKOV- 3, HepG- 2 and MGC- 803 in vitro. Methods: Alkali extraction and alcohol precipitation were employed to extract the
    antitumor activity of effective components HTKD(D), HTKE(E)and HTKF(F)from Juglans sigillata shell and observe the inhibitory
    effects with MTT assays. Results: At highest concentration component E could obviously inhibit the growth of three human tumor cells
    with dose-dependence. In addition, component E inhibited the three tumor cell lines with minimal the median inhibitory concentration
    (IC50). Conclusion: The extract of Juglans sigillata shell showed anti-tumor effects and pharmacodynamic material mainly exist in the
    component E.

    Clinical Study on Non-alcoholic Fatty Liver Disease and Acute Coronary Syndrome
    Xiong Liping, Liu Zhengxing
    2015, 14(8):  18-21. 
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    Objective: To study the correlation of non-alcoholic fatty liver disease(NAFLD)and acute coronary syndrome(ACS)
    pathogenesis. Methods: One hundred and forty cases of ACS with NAFLD hospitalized in our hospital from January 2006 to December
    2013 were divided into NAFLD group(n=80)and non NAFLD group(n=60), the results were compared by vascular ultrasound
    detection rate of carotid plaque. ACS was divided into three clinical subgroups of unstable angina pectoris(UA), acute ST segment
    elevation myocardial infarction(STEMI), non ST segment elevation acute myocardial infarction(NSTEMI). And the incidence of
    NAFLD was observed in each subgroup. Riglyceride(TG), cholesterol(TC), low density lipoprotein(LDL- C) levers have no
    difference in both NAFLD group and non NAFLD group. Results: The detection rate of carotid plaque in ACS combined with NAFLD
    group was significantly higher than that in non NAFLD group(85% vs 40%), there was significant difference between two groups(P<
    0.01); there was no statistically significant in the three subgroups of ACS the incidence of NAFLD and non NAFLD incidence rate
    difference(P > 0.05); group NAFLD, TG, TC, LDL-C level was significantly higher than that of non NAFLD group(P < 0.05).
    Conclusion: NAFLD patients have high risk carotid atherosclerosis and hyperlipidemia appears, which is a potential risk factor for
    coronary heart disease ACS, timely intervention of NAFLD patients can be benefit in the prevention and treatment of cardiovascular
    diseases.

    Clinical Analysis and Literature Review of Six Cases of Intestinal Mantle Cell Lymphoma
    Zhu Weimei, Li Shali
    2015, 14(8):  22-24. 
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    Objective: To improve the awareness of intestinal mantle cell lymphoma. Methods: Six clinical cases which were
    identified as intestinal mantle cell lymphoma through colonoscopy and immunohistochemistry by the First Affiliated Hospital of Sun
    Yat-sen University from May 2008 to June 2012 were retrospectively analyzed. Results: It is clear that a significant percentage of
    intestinal mantle cell lymphoma occurs in old aged male. Multiple lymphomatous polyposis has been found in intestine under
    colonoscopy. Furthermore, immunohistochemistry presents that most all cases express T-cell associated antigen CD5, and over express
    cycling D1. At the same time, some cases also express CD43, while others show the negative CD23 and CD10. Conclusion: Intestinal
    mantle cell lymphoma is a refractory lymphoma with limited curative effect. Biopsy specimen by colonoscopy and process
    immunophenotype is an important diagnosis method.

    Clinical Observation of Bronchoalveolar Lavage in the Treatment of 92 Cases with Severe Pneumonia
    Li Sheng, Mei Tonghua
    2015, 14(8):  25-27. 
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    Objective: To observe clinical effects of bronchoalveolar lavage in the treatment of severe pneumonia. Methods: 92
    patients with severe pneumonia were divided randomly into two groups. Control group received routine treatment, patients in the
    treatment group based on the traditional treatments performed bronchoalveolar lavage treatment. Clinical efficacy and hospitalization
    days were compared and analyzed between the two groups. Results: The total effective rate of observation group was significantly
    higher than that of the conventional treatment control group. Total effectiveness of treatment group was 95.7% , while the total
    effectiveness of control group was 84.4%. The difference of clinical symptoms, imaging from the absorption of inflammation, white
    blood cells of normal time was statistically significant(P<0.05); hospitalization days of treatment group was significantly shorter than
    the control group, respectively(14.2±3.2)d and(19.4± 4.3)d. Conclusion: Bronchoalveolar lavage is a safe, effective method in the
    treatment of severe pneumonia, the clinical efficacy is significant, worthy of promotion and application.

    Compararison of Nuss Procedure and Modified Ravitch Procedure for Pectus Excavatum Repair
    Li Na, Wang Ning, Zhang Shanshan, Zhang Mudan, Bijay Upadhyay, Wu Xuedong
    2015, 14(8):  28-31. 
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    Objective: To compare the results of Nuss procedure and modified Ravitch procedure in the management of pediatric
    pectus excavatum. Methods: The clinical data of 26 cases who received pectus excavatum repairs were analyzed retrospectively and
    they were divided into Nuss group in 10 and modified Ravitch group in 16 according to the procedure performed on them. The lasting
    time and amount of bleeding in operation, average hospital days and complications were compared between two groups statistically.
    Results: Operation was performed on all patients successfully and satisfied thoracic appearance obtained. The average age is no
    difference compared between two groups. The operative time was shorter and less bleeding in Nuss group than that in modified Ravitch
    group(P <0.05)but no statistical significance hospital days and complications compared between two groups(P >0.05). Conclusion:
    Satisfactory orthopedic effect can be obtained whether Nuss procedure or modified Ravitch procedure for pectue excavatum repair but
    Nuss procedure with the advantages of shorter operative time, less trauma and less bleeding in operation.

    Clinical Observation of Pelvic Floor Rehabilitation Used in the Prevention of Postpartum Stress Urinary
    Incontinence
    Wu Xiaorong
    2015, 14(8):  32-34. 
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    Objective: To observe the clinical curative effect of pelvic floor rehabilitation used in the prevention of postpartum stress
    urinary incontinence and discuss its application value. Methods: Eighty-six cases of pregnant women in our hospital from January
    2010 to December 2012 were divided into observation group and control group, 43 cases in each group. Observation group used pelvic
    floor muscle rehabilitation apparatus treatment, while control group used conventional postnatal health training. We compared two
    groups in postpartum urinary incontinence and pelvic floor muscle changes after 2 months and 9 months. Results: The incidence of
    urinary incontinence of the observation group was obviously lower than that of the control group after rehabilitation training. The
    difference had statistical significance(P <0.05). The pelvic floor muscle strength of the observation group improved more than that of
    the control group after the treatment. The difference had statistical significance(P <0.05). Conclusion: Application of pelvic floor
    muscle rehabilitation apparatus for pelvic floor rehabilitation training can effectively prevent the occurrence of postpartum stress
    urinary incontinence. And it can reduce the incidence of urinary incontinence. Furthermore, it has no side effect and is safe and
    effective. So it is worthy of further clinical research.

    Risk Analysis of the Chronic Mountain Sickness(CMS)in Healthy Population living in the Western Yunnan Plateau
    Zi Yunhai, Zi Jianwen, Li Qian, Zhang Yuling, Tang Hui, Gong Nianting
    2015, 14(8):  35-40. 
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    Objective: To investigate the CMS risk of healthy population in western Yunnan plateau. Methods: CMS risk of healthy
    population living at different districts was analyzed in 5 ways: bone density measurement, blood routine examination, hemorheological
    analysis, blood biochemistry and immunologic function. Results: The bone density of people gradually increased with elevation. RBC
    and Hb gradually increased and blood viscosity increased, the difference was statistically significant(P<0.05). Except for TP, Alb
    and Glu, other indexes of liver function increased with elevation. Indexes of renal function and myocardial enzymes increased as
    altitude rose(P<0.05). The TC, TG, LDL-C, APoB level of Dali and Weixi group were significantly lower than those of Shangri-La
    group(P<0.05), and these were even lower than Deqin group(P<0.05). The IgG, IgM, IgA, C3, C4 and CD3+, CD8+ level of Dali
    and Weixi group were significantly lower than Shangri- La group(P<0.05), and were still lower than Deqin group(P<0.05).
    Conclusion: The indexes of bone density measurement, blood routine examination, hemorheological analysis, blood biochemistry and
    immunologic function of healthy people varied differently, and the CMS risk gradually increased.

    Clinical Observation of Baoshen Decoction Treating Chronic Nephritis Caused by Spleen-Kidney Deficiency
    Tang Zhenli, Qiu Jianghong
    2015, 14(8):  41-44. 
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    Objective: To observe the treatment of Baoshen decoction on chronic nephritis caused by spleen-kidney deficiency.
    Methods: Sixty patients with chronic nephritis caused by spleen-kidney deficiency diagnosed by traditional Chinese medicine(TCM)
    were randomly divided into treatment group(30 cases)and control group(30 cases). Each group was given conventional Western
    medicine treatment: high quality low protein diet, irbesartan tablets(75 mg qd). In addition, the treatment group orally took Baoshen
    decoction 200 mL qd for 4 weeks. The proteinuria, TCM syndromes, efficacy of TCM syndrome before and after treatment were
    compared. Results: Proteinuria decreased after the treatment in both groups, the difference was statistically significant(P <0.05). And
    the proteinuria of treatment group was much improved than the control group, the difference was statistically significant(P < 0.05).
    Conclusion: Baoshen decoction has an outstanding curative effect for chronic nephritis caused by spleen-kidney deficiency.

    Comparative Analysis of Imaging Diagnosis and Pathological Diagnosis in Choroid Plexus Papilloma
    Jiang Chaomei, Luo Lin, Chen Guoyun, Chen Xiaodong
    2015, 14(8):  45-48. 
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    Objective: To investigate the phenotypes of choroids plexus papiloma in CT, MRI and comparatively analyze them with
    pathologic manifestations so as to understand this disease. Methods: A retrospective analysis of CT and MRI phenotype was applied in
    19 cases of choroids plexus papilloma, confirmed by surgery and pathology, among which 5 cases were checked with CT, 10 with MRI,
    4 with CT and MRI. Image finding was compared with pathological result. Results: There were 13 cases of tumor in the fourth
    ventricle, 5 in the lateral ventricle, 1 in cerebellum pontine angle;there were 18 cases of papillary tumor margins or ovulated, 13 cases
    of tumor enhancement after internal mulberry-like or granular enhanced in MRI; And there were 6 cases of tumor lesions calcification,
    2 of hemorrhage, 18 of hydrocephalus. There were 14 cases of tumor diagnosis choroids plexus papilloma(grade I), 5 cases diagnosis
    atypical choroids plexus papilloma(II level)in pathological diagnosis. Conclusion: A close link exists in imaging performance and
    pathological features of choroids plexus papilloma, pathological features could help us understand imaging performance.

    Expressions and Related Studies of Survivin and OPN in Different Cervical Lesions
    Wang Yanshu, Lu Jing, Li Haiyan
    2015, 14(8):  49-52. 
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    Objective: To discuss the expressions of Survivin and OPN in the tissues of chronic cervitis, cervical intraepithelial
    neoplasia(CIN)and cervical squamous carcinoma. Methods: Immunohistochemical SP method was applied to detect the expressions
    of Survivin and OPN in 10 cases of chronic cervicitis, 20 cases of CIN and 50 cases of cervical cancer. Results: The expression
    positive rate of Survivin and OPN in chronic cervicitis、CINⅢ、cervical squamous carcinomas increased with the lesion severity level
    enhancement,three groups of comparative differences are statistically significant(P<0.05). The expression positive rate of Survivin
    in cervical squamous carcinomas was associated with clinical stages and pathologic stage(P <0.05), but has nothing to do with age and
    lymph nodes metastasis(P >0.05). The expression positive rate of OPN in cervical squamous carcinomas was associated with clinical
    stages and lymph node metastasis(P < 0.05), but has nothing to do with age and pathologic stage(P > 0.05). Conclusion: The joint
    detection of Survivin and OPN may serve as the index for early diagnosis, condition monitoring and prognosis judgment of cervical
    squamous carcinomas.

    Analysis of the Epidemiological Features of Pulmonary Tuberculosis in Heqing County from 2005 to 2013
    Zhang Xuehua, Zhao Jinhua,Duan Huafen
    2015, 14(8):  53-56. 
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    Objective: To find out the epidemiological features of pulmonary tuberculosis in Heqing County from 2005 to 2013 and to
    provide basis for a further control of the outbreak. Methods: Descriptive epidemiological method was applied to analyze the TB cases
    registered during 2005 and 2013. Results: One thousand one hundred and eighty TB cases were registered in the whole county during
    2005 and 2013. Male patients were 1.97 times of women patients; and it focused on people at 25 to 64 years old(71.2%), peaks at 45
    to 54 years old, rural patients topped the list(88.22%); elderly population above 65 years old accounted for 17.2%, students patients
    ranked second(3.73%). Conclusion: Rural population is still the focus of TB control in Heqing County. And meanwhile TB should be
    controlled and surveyed at school and among elderly population.

    Cis-Position Analysis of Residents' Death Causes at Binchuan County in 2013
    Duan Pingxian
    2015, 14(8):  57-60. 
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    Objective: To analyze the death causes and cis-position of residents at Binchuan County in 2013, and to provide a
    scientific basis for the prevention and control measures. Methods: The death causes were classified according to International
    Classification of Disease ICD-10, and then were statistically analyzed by the death surveillance data cleansing and analysis software
    and Excel2003. Results: ①The mortality rate at Binchuan County in 2013 was 630.89/100 000. Male mortality was 725.72/100 000,
    female mortality was 531.74/100 000. The ratio of male to female was 1.36:1. ②The top 5 causes of death were circulatory system
    diseases, respiratory system diseases, injuries, malignant tumors and digestive system diseases, accounting for 89.25% of the total
    death toll. Conclusion: Chronic diseases were the main cause of death at Binchuan County, and chronic non-communicable diseases
    were the major death cause. Therefore the prevention and intervention of chronic diseases are the focus of future work.

    MBI Test of Nurses at Minority Districts
    Fu Yanfen, Wang Jianhua, Gao Xueyan, He Yinlai, Yang Hongfei
    2015, 14(8):  61-64. 
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    Objective: To test the reliability and validity of the Chinese version of Maslach Burnout Inventory(MBI)in nurses at
    minority districts. Methods: MBI was tested by 592 nurses in Dali Prefecture to evaluate the reliability and validity. Results: Factor
    analysis showed that the item of diminished personal accomplishment was in accordance with the original scale, but item 6, 16, and 20
    in the original scale were moved from emotional exhaustion dimension to depersonalization. The reliabilities of internal consistency of
    the inventory was 0.834, and the three dimensions(depersonalization,emotional exhaustion,sense of achievement)ranged from 0.787
    to 0.807. The correlation between each item and its dimension was stronger than the item with other dimensions. Conclusion: The
    reliability of the Chinese version of MBI is acceptable. But the construct is different from the original inventory. So if we want to
    investigate the nurses in minority district, we have to revise the scale firstly.

    Effect Research of Integrated Health Care Model Reducing Deep Vein Thrombosis in Patients with
    Spine Surgery
    Chen Li, Zhang Zhipeng
    2015, 14(8):  65-69. 
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    Objective: To discuss the effect of integrated health care model reducing the incidence of postoperative deep vein
    thrombosis(DVT)in patients with spine surgery. Methods: Integrated health care group was set up and management procedure was
    made. The reasons and countermeasures of deep vein thrombosis caused by spine surgery were analyzed. Doctors and nurses
    participated in training with clear responsibility and mutual assistance. The incidence of postoperative DVT before and after this new
    model was compared. Results: The incidence of DVT after anterior spinal surgery was 7.19%, which after two rounds of quality
    improvement was reduced to zero. The difference before and after was statistically significant(P < 0.05). Conclusion: Integrated
    health care model can reduce the incidence of spinal surgery patients with DVT and improve the quality of medical care.

    Application of QCC in Reducing the Incidence Rates of the Falling of Inpatients
    Li Rutian, Dong Fangping
    2015, 14(8):  70-73. 
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    Objective: To study the effect of QCC in reducing the incidence rates of the falling of inpatients. Methods: QCC was
    founded by volunteers. The theme of the activity was made and the plane was settled. Sixteen falling cases happened in the internal
    medicine department at a hospital in Dali from January 2013 to January 2014 were analyzed. The correlates of incidence were mainly
    studied and the countermeasures were proposed. Then the incidence rates before and after the activity were compared to evaluate the
    effect and establish a standardized management. Results: After the activity, the incidence rates of falling of inpatients decreased from
    0.009% to 0.005%. Conclusion: The application of QCC can reduce the incidence rates of falling of inpatients and play a remarkable
    positive role in promoting the group cohesiveness and abilities of QCC members in solving problems.

    Experience of Rescuing the Batch Wounded of Serious Incidence of Violence and Terrorism
    ——A Case Study of Kunming "301" Violent Terrorist Event
    Yuan Yicun
    2015, 14(8):  74-76. 
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    Objective: To review the successful experience of rescuing the batch wounded in Kunming "301" serious incidence of
    violence and terrorism so as to improve the efficiency of emergency treatment. Methods: The emergency management of unexpected
    public event was launched immediately. The batch wounded were rescued and treated quickly. Results: All patients were rescued and
    treated in 15 to 60 minutes after arriving at the emergency department, which had provided a powerful life channel for emergency
    operations and other further treatments and achieved good results. Conclusion: Third class A level hospital deals with the unexpected
    public events orderly, timely and efficiently.

    Perioperative Nursing of 36 Cases of Laparoscope-assisted Radical Gastrectomy for Gastric Cancer
    Yang Chunfang
    2015, 14(8):  77-79. 
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    Objective: To discuss the characteristics of perioperative nursing of laparoscope-assisted radical gastrectomy for gastric
    cancer. Methods: The treatment and perioperative care of 36 cases of laparoscope-assisted radical gastrectomy for gastric cancer were
    retrospectively analyzed. Results: Except for 5 cases of short - term complications after conservative treatment in 36 cases of
    laparoscopic surgery which were later cured by conservative treatment, others had no complications of surgery and perioperative care.
    Conclusion: The perioperative management can greatly improve the success rate and reduce the incidence of complications,
    especially, it has important implications for the rehabilitation of patients and complications of preventive care.

    Nursing Care of PPH Operation Treating 108 Cases of Hemorrhoids in Township Health Centers
    Su Liping, Guo Aimei
    2015, 14(8):  80-82. 
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    Objective: To explore the new nursing method of PPH operation treating hemorrhoids applied by township health centers
    and to regulate and improve the quality of nursing. Methods: Preoperative and postoperative health education manuals were made to
    regulate nursing and prevent complications. Results: One hundred and eight cases of PPH patients received satisfactory nursing. Anal
    pain and distention occurred in 15 cases, urinary retention occurred in 44 cases after operation. Others had no anal incontinence, anal
    stenosis, anal infection and other complications. Conclusion: Health education manual which standardizes nursing management is
    suitable for township health centers to improve nursing quality.

    Countermeasure Research Based on the Entrance Education to Cultivate Highly Qualified Nurses
    Chai Shixue
    2015, 14(8):  83-86. 
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    Objective: To discuss the purpose, significance and form of entrance education for freshmen majoring in nursing.
    Methods: The entrance education practiced by the Nursing College of Dali University in recent years was summarized and analyzed.
    Results: The entrance education for freshman could combine collective education with individual education, or general education with
    subject- based education to effectively help students know their college and specialty. Through this, freshman may have a clear
    academic goal, correct and stable faith in their major. Besides, freshman could get good habits so that they could adapt to university
    study and life as soon as possible from their secondary school days. All these will build a sound foundation for students to successfully
    complete their study in the university. Conclusion: It's essential that all staff should recognize the importance of entrance education.
    Only when staffs willingly devote to the entrance education for freshman could it become efficient.

    Reform and Research on Practice Teaching System of Four-year Undergraduate Nursing Specialty
    Zhao Yuan, Liao Jianying, Zhang Xiaolan
    2015, 14(8):  87-89. 
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    Objective: To construct the practice teaching system of four-year undergraduate nursing specialty. Methods: Under the
    guidance of humanism, it constructed the practice teaching system by curriculum construction, teaching reform, research, practice
    management, student work, etc. Results: It has constructed the practice teaching system with nursing professional characteristics
    of "four -in -one, four -platform, three -level, six -link". Conclusion: Nursing specialty of Dali University is the characteristic
    specialized construction of ministry of education. Construction of the practice teaching system with nursing professional characteristics
    of four-year undergraduate nursing specialty can provide a reference significance for the construction of the practice teaching system of
    undergraduate nursing in Yunnan higher education and even the national higher education.

    Application of Network-Assisted Teaching in Human Anatomy Teaching
    Wang Yong, Zhang Xiujun
    2015, 14(8):  90-92. 
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    Objective: To discuss the influence of network-assisted teaching on the study and examination of anatomy. Methods: A
    network tests system of human anatomy was built. 2012 Grade students of clinical medicine were taken as the experimental group
    which was compared with the control group, students of 2013 Grade. Students could do on-line tests any time one week before the final
    examination; the highest score will be included in the curriculum of each student grade. Questionnaire was conducted to the
    experimental group. The scores of each group were statistically analyzed. Results: Most students used on-line resource effectively to
    intensify the learning thus having well prepared for the examination and improved their comprehensive abilities. The scores of the
    experimental group students is better than the control group by contrasting the scores in the curriculum examination of the two groups
    students(P <0.05). Conclusion: Students effectively used the on-line tests to prepare for the examination and improved test scores.