Abstract:

Objective: To explore the chemical constituents from the underground part of Isodon lophanthoides. Methods: The
compounds were isolated and purified by silica gel and Sephadex LH-20 column chromatography. Their structures were identified by
various spectral analysis and physicochemical properties. Results: Five steroidal compounds and two ceramide compounds were
obtained from ethyl acetate fraction of 70% acetone-water extract of the Isodon lophanthoides underground part and identified as
stigmast-4-ene-3-one（1）, stigmasterol（2）, 7α-hydroxysitosterol（3）, daucosterol（4）, 5α, 6β-dihydroxy-daucosterol（5）,（2S, 3S,
4R, 8E）-2-［（2′R）-2-hydroxytetracosanoylamino］-1, 3, 4-octadecanetriol-8-ene（6）, and 1-O-β-D-glucopyranosyl-（2S, 3R, 4E,
8Z）-2-［（2′R）-2-hydroxytetracosanoylamino］-1,3-octadecanediol-4, 8-diene（7）. Conclusion: All of these compounds except for
2 and 4 were isolated from this plant for the first time, while the compounds 5-7 were firstly obtained from the underground part of
Isodon plants.

Key words: Isodon lophanthoides, underground part, steroids, ceramides