Effects of Fecal Sample Preservation Methods on the Monitoring of Intestinal Oesophagostomum in
Rhinopithecus bieti

›› 2017, Vol. 2 ›› Issue (12): 70-73.

Effects of Fecal Sample Preservation Methods on the Monitoring of Intestinal Oesophagostomum in Rhinopithecus bieti

（1. Institute of Eastern-Himalaya Biodiversity Research, Dali University, Dali, Yunnan 671003, China; 2. Collaborative Innovation Center for the Biodiversity in the Three Parallel Rivers of China，Dali, Yunnan 671003, China; 3. Administration of Baimaxueshan National Nature Reserve, Diqing, Yunnan 674609, China; 4. Southwest Forestry University, Kunming 650224, China）

Received:2017-08-25 Online:2017-12-15 Published:2017-12-15

Abstract

Abstract: Objective: To explore an effective fecal sample preservation method for quantitative monitoring of intestinal Oesophagostomum in Rhinopithecus bieti. Methods: The detection rate and detection intensity of Oesophagostomum in Rhinopithecus bieti fecal samples from two preservation methods（formalin preservation and cryopreservation）were compared and analyzed, and the sample preservation under different storage conditions was analyzed for different effects. Results: The samples stored at -20 ℃ showed that the detection intensity of Oesophagostomum eggs was（919.69 ± 916.09）/ g, the detection rate was 88.89%（56/63）, the detection rate did not decline within 30 days, and the detected intensity also maintained at a high level. The sample stored in formalin reduced its intensity to 4.98% on 4th day, and the detection intensity and detection rate showed significant differences（t=6.799, P <0.001, df=62; χ2=45.10, P<0.01）. Conclusion: In a frozen condition at -20 ℃ which ensures the detection rate and intensity of Oesophagostomum, the storage time of the fecal samples of Rhinopithecus bieti can be effectively prolonged.

Key words: Rhinopithecus bieti, fecal samples, preservation method, detection rate, detection intensity

CLC Number:

Li Yanpeng, Huang Zhipang, Liu Shuoran, Yang Yimei, Zhong Tai, Su Xijun,. Effects of Fecal Sample Preservation Methods on the Monitoring of Intestinal Oesophagostomum in Rhinopithecus bieti[J]. , 2017, 2(12): 70-73.

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