Study on the Compounds with α-Glucosidase Inhibitory Activity from Roots of Astragalus englerianus

Abstract

Abstract:

Objective: To study the chemical components of Astragalus englerianus, and to explore the active compounds of α-
glucosidase's inhibiting from the roots of Astragalus englerianus. Methods: The compounds were isolated and purified by Rp-18,
Sephadex LH-20 and silica gel column chromatography. Their structures were identified by modern spectroscopy techniques.
The α -glucosidase's inhibiting experiment was performed by using enzyme inhibitor screening model with 4-nitrophenol α -
D-glucopyranoside（PNPG）as the substrate. Results: Six compounds were isolated from ethyl acetate fraction of methanol extract
of the roots of A. englerianus and identified as 1- O- hexadecanolenin（1）, 2,4'- dihydroxy- 4- methoxy dihydrochalcone（2）,
daucosterol（3）, 5α,6β- dihydroxy- daucosterol（4）,（3R）- vestitol- 7- O- glucoside（5）, and（2S,3S,4R,8E）- 2-［（2'R）- 2-
hydroxytetracosanoylamino］- 1,3,4- octadecanetriol- 8- ene（6）. All of these compounds were examined for their α- glucosidase
inhibitory activities. As a result, compound 2 showed significantly activity with the lower IC50 than the positive control, acarbose.
Compound 5 and its aglucon,（3R）-vestitol also showed some inhibitory activity. Conclusion: All of the compounds were isolated from
the roots of A. englerianus for the first time, and the flavonoids showed the better α-glucosidase inhibitory activity than the other
compounds.

Key words: Astragalus englerianus, root, compounds, α-glucosidase, inhibitory activity

CLC Number:

R932

Qiu Lin, Xiao Chaojiang, Dong Xiang, Jiang Bei. Study on the Compounds with α-Glucosidase Inhibitory Activity from Roots of Astragalus englerianus[J]. , 2017, 2(4): 1-5.

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