Abstract:

Objective: To explore the experimental conditions for the L-form of Candida albicans induced by different antifungal agents. Methods: The L-form of Candida albicans was induced by the common antifungal agents according to the macro-liquid dilution method. The fungal cells treated with drugs were taken out for observation under microscope every day, and then for culture in SDA media. Results: After 4-day induction by using fluconazole（2 μg/mL）and amphotericin B（0.5 μg/mL）treatment, the L-form of Candida albicans can be seen under oil lens. The suspected L-form cells could pass through the bacterial filter（0.8 μm）and grow under the conditions of high osmotic pressure. Under the same condition, none of the L- form cell was observed after 7 days of fluorocytosine treatment, and they could not grow through the filter. Conclusions: The L-form of Candida albicans can be artificially induced by using some antifungal agents when the conditions are suitable.

Key words: L-form cell, Candida albicans, antifungal agents, experimental induction