A Study of the Association Between 3'UTR Polymorphisms of NRAMP1 Gene and Susceptibility to Pulmonary Tuberculosis Among Bai Nationality

Abstract

Abstract:

Objective: To explore the correlation between the genetic polymorphism of NRAMP1 3'UTR and the susceptibility to pulmonary tuberculosis of Bai nationality in Dali. Methods: PCR-RFLP was used to analyze NRAMP1 3'UTR polymorphism of 191 TB-positive and 193 healthy people of Bai nationality living in Dali in order to study whether the gene polymrphism was correlated with the susceptibility to pulmonary tuberculosis among Bai nationality. A follow- up study was also conducted after one- month treatment to analyze whether the curative effects of treatment was correlated with the gene polymorphism. Results: Both AA and GA at NRAMP1 3'UTR（OR=2.208 and 3.373, 95% CI=［1.343-3.630］and［1.114-10.210］）were risk factors for a higher TB morbidity among Bai people. And the A allele was positively correlated with the pulmonary tuberculosis morbidity of Bai nationality（OR=2.219, 95% CI=［1.470-3.349］）. However, there was no statistically significant difference among the side -effect kinds and degrees after antituberculosis therapy（P＞0.05）. Conclusion: The polymorphism of NRAMP1 3'UTR is possibly associated with the susceptibility to pulmonary tuberculosis among Bai nationality of Dali. However, the side-effects of antituberculosis therapy do not appear to be
related to the genetic polymorphism of NRAMP1 3'UTR.

Key words: NRAMP1 gene, Bai nationality of Dali, pulmonary tuberculosis, gene polymorphism

CLC Number:

R743.32

WANG Gui-Hua, HE Lian-Fu. A Study of the Association Between 3'UTR Polymorphisms of NRAMP1 Gene and Susceptibility to Pulmonary Tuberculosis Among Bai Nationality[J]. , 2018, 3(4): 64-68.

References

〔1〕WORLD HEALTH ORGANIZATION. Global Tuberculosis Report2016〔C〕. Global Tuberculosis Report，2016.

〔2〕WU L S H，LEE S W，HUANG KY，et al. Systematic Expression Profiling Analysis Identifies Specific MicroRNAGene Interactions that May Differentiate between Active and Latent Tuberculosis Infection〔J〕. Biomed Res Int，2014：895179.

〔3〕DE MAGEE M J，SUN Y V，BRUST J C M，et al. Polymorphisms in the vitamin D receptor gene are associated with reduced rate of sputum culture conversion in multidrug-resistant tuberculosis patients in South Africa〔J〕. PLoS ONE，2017，12（7）：e0180916.

〔4〕DE KNEGT G J，DICKINSON L，PERTINEZ H，et al. Assessment of treatment response by colony forming units，time to culture positivity and the molecular bacterial load assay compared in a mouse tuberculosis model〔J〕. Tuberculosis，2017，105（5）：113-118.

〔5〕YUAN L Y，KE Z Q，GUO Y，et al. NRAMP1 D543N and INT4 polymorphisms in susceptibility to pulmonary tuberculosis：A meta-analysis〔J〕. Infect Genet Evol，2017，54：91-97.

〔6〕WU Y Y，GUO Z K，LIU F Y，et al. Sp110 enhances macrophage resistance to Mycobacterium tuberculosis via inducing endoplasmic reticulum stress and inhibiting anti-apoptotic factors〔J〕. Oncotarget，2017，8（38）：64050-64065.

〔7〕ZHANG J W，ZHAO Z Z，ZHONG H Y，et al. Importance of common TLR2 genetic variants on clinical phenotypes and risk in tuberculosis disease in a Western Chinese population〔J〕. Infect Genet Evol，2018，60：173-180.

〔8〕TIWARI V，KEDIA S，GARG S K，et al. CD4 + CD25 + FOXP3+ T cell frequency in the peripheral blood is a biomarker that distinguishes intestinal tuberculosis from Crohn's disease〔J〕. PLoS ONE，2018，13（2）：e0193433.

〔9〕MEDAPATI R V，SUVVARI S，GODI S，et al. NRAMP1 and VDR gene polymorphisms in susceptibility to pulmonary tuberculosis among Andhra Pradesh population in India：a case-control study〔J〕. BMC Pulm Med，2017，17：89.

〔10〕FERNÁNDEZ-MESTRE M，VILLASMIL Á，TAK-IFF H，et al. NRAMP1 and VDR Gene Polymorphisms in Susceptibility to Tuberculosis in Venezuelan Population〔J〕. Disease Markers，2015，2015：860628.

〔11〕BELLAMY R，RUWENDE C，CORRAH T，et al. Assessment of the interleukin 1 gene cluster and other candidate gene polymorphisms in host susceptibility to tuberculosis〔J〕. Tuber Lung Dis，1998，79（2）：83-89.

〔12〕安雅臣，冯福民，袁聚祥，等. NRAMP1 基因INT4 和3'UTR位点多态性与肺结核易感性的研究〔J〕. 中华流行病学杂志，2006，27（1）：37-40.

[1]	Li Wenwu, Lü Xiujuan. CYP2C19 Gene Polymorphism in Patients with Cerebral Infarction in Chuxiong Area of Yunnan Province [J]. Journal of Dali University, 2020, 5(8): 48-51.
[2]	SHU Hui-Qiong, HUANG Gong-Li, MO Rong, LI Meng-Wu. Analysis of Nutritional Intervention on 119 Patients Suffering from Pulmonary Tuberculosis Complicated with Intestinal Tuberculosis [J]. , 2019, 4(4): 48-51.
[3]	Zhou Li, Chen Haiyan, Kong Shan, Cheng Jiamao. Associations of IFN-γ Gene Polymorphism with Dilated Cardiomyopathy [J]. , 2017, 2(8): 28-30.
[4]	. Analysis of Chest X-rays on Chronic Psychoses Infected by Pulmonary Tuberculosis [J]. J4, 2012, 11(3): 64-66.
[5]	. Pathogens in Pulmonary Tuberculosis Complicated Low Respiratory Tract Infection：The Distribution and Antimicrobial Resistance Analysis of 489 Strains [J]. J4, 2011, 10(6): 36-38.