Abstract: 〔Abstract〕Objective: To study the effect of scutellarin on the expression of CYP2C9 in Chang Liver cells. Methods: The effect of
scutellarin on proliferative activity of Chang Liver cells was detected by MTT assay. The relative expression of CYP2C9 mRNA and
protein was detected by RT- PCR and Western- blot respectively. Results: MTT results showed that scutellarin inhibited the
proliferation of Chang Liver cells in a concentration-dependent manner, and the half inhibitory rate（IC50）was 1.87 mmol/L. RT-PCR
results showed that the expression of CYP2C9 mRNA significantly decreased in 5.0, 10.0, 20.0, 40.0 μmol/L scutellarin groups（P<
0.05）, compared with DMSO group. Western- blot results showed that the expression of CYP2C9 protein in 20.0, 40.0 μmol/L
scutellarin groups significantly decreased（P<0.05）, compared with DMSO group. Conclusion: Scutellarin has different degree of
inhibitory action on the expression of CYP2C9 mRNA and protein in Chang Liver cells.

Key words: 〔Key words〕scutellarin, CYP2C9, mRNA expression, protein expression, Chang Liver cells