Abstract: Objective: To understand the stabilities and disinfection effects of main effective ingredients in two brands of disposable
disinfection solutions on the market. Methods: Using ultraviolet spectrophotometry and gas chromatography to determine the
relationship between the residues of the main effective components, i.e. ethanol and polyhexamethylene biguanide hydrochloride, and time after the use of two kinds of disposable disinfectants. At the same time, field disinfection experiments were also carried out to monitor the sterilizing rate of disinfectant in different periods after use. Results: Within 100 minutes, the content of ethanol in two
kinds of disinfectants decreased by 83.10% and 58.83% respectively, while the concentration of polyhexamethylene biguanide
hydrochloride increased by 267.94% and 88.57% respectively. Field disinfection experiments showed that both disinfectants had very
high sterilizing rate（99.89%, 99.75%）in the first 40 minutes. Disinfectant A had the best germicidal effect at 20 minutes, and
disinfectant B had the best germicidal effect at 40 minutes. Disinfectant A had the germicidal effect decreased obviously at 60 minutes,while disinfectant B remained at a high level. Conclusion: Comparatively speaking, the disinfection effect of disinfectant B is slightly lower than disinfectant A, but the composition of disinfectant B is more stable and the germicidal maintenance time is longer than disinfectant A.

Key words: disposable disinfectant solution, disinfection effect, component determination, stability